Biological activity of alginate and its effect on pancreatic lipase inhibition as a potential treatment for obesity

Abstract

Alginates are classed as a dietary fibre and have been shown to inhibit digestive enzymes in vitro, and therefore could be used as an obesity treatment. The current study aims to assess whether alginate in a bread vehicle maintains its inhibition properties despite cooking and digestion, and may therefore be used as a potential treatment for obesity. After 180 min in a model gut that replicates digestion in the mouth, stomach and small intestines alginate bread (AB), control bread (CB), CB with Manucol® DM alginate, free DM alginate and model gut solution were collected. DM, LFR 5/60 and SF200 were heated at 37 °C and 200 °C, with DM also heated at 50, 100 and 150 °C. Samples from the model gut and heated alginate were assessed for molecular size and inhibition properties using viscosity, gel filtration and a lipase turbidity assay. AB does not significantly increase viscosity in the model gut. Viscosity of alginate reduces beyond 100 °C, although alginate retains its inhibition properties up to 150 °C. Cooking into the bread does not reduce the molecular size of the alginate or affect its inhibition properties. These data demonstrate the robustness of alginates lipase inhibition despite the cooking process and digestion. Therefore adding alginate to a bread vehicle may have the potential in the treatment for obesity.

Influence of Hydrocolloids (Dietary Fibers) on Lipid Digestion of Protein‐Stabilized Emulsions: Comparison of Neutral, Anionic, and Cationic Polysaccharides

“…This may be explained by the gelation of alginate by calcium ions discussed above (Tonnesen and Karlsen ; Hu and others ; Li and McClements ). An increase in the viscosity of the intestinal fluids may reduce the efficiency of mixing and diffusion, and thereby decrease lipase activity by hampering its ability to come into contact with the lipid droplet surfaces (Dikeman and Fahey ; Houghton and others ), which could have a profound influence on the physiological fate of ingested lipid droplets.…”

Effects of (-)-epigallocatechin 3-O -(3-O -methyl) gallate (EGCG3″Me)- phospholipids complex on pancreatic α-amylase and lipase activities

“…Generally, the excess digestion of carbohydrates and fat is an essential step in gaining weight, therefore, reducing or slowing the digestive availability of calories is considered to be useful in the prevention of obesity. Furthermore, it has been reported that inhibitors of pancreatic α‐amylase and lipase are useful for slowing down the digestion of starch and fat (Fei et al, ; Houghton et al, ). Thus, the development of an effective and nontoxic dietary regimen has important significance for the prevention and treatment of obesity.…”

Genetic, structural and pharmacological characterization of polymannuronate synthesized byalgGmutant indigenous soil bacteriumPseudomonas aeruginosaCMG1421

“…) by protonating active sites in enzyme's structure (Houghton et al . ). Many polysaccharides including alginate have interesting potentials for treatment of various diseases.…”