Biological activity evaluation of the oils fromLaurus nobilisof Tunisia and Algeria extracted by supercritical carbon dioxide

Abstract

Isolation of essential oil from dried and ground leaves of Laurus nobilis L. from Algeria and Tunisia were obtained with two different methods: by means of classical hydrodistillation (HD) and by using carbon dioxide in the supercritical state (SFE). The effect of extraction pressure on the yield and composition of the L. nobilis volatile oil is examined. The best extraction conditions were as follows: pressure, 9.0 MPa; temperature, 323.15 K; 4 h of extraction and carbon dioxide flow, Phi = 1.2 kg h(-1). Waxes were entrapped in the first separator set at 9.0 MPa and 263.15 K. The oil was recovered in the second separator working at 2.0 bar and 288.15 K. GC/MS analysis of the leaves' volatile oil revealed that it mainly consisted of: 1,8-cineole, linalool, alpha-terpinyl acetate, methyl eugenol and sabinene. The comparison with the hydrodistilled oil did not reveal any big difference. The collection of samples at different extraction times during supercritical extraction allowed us to monitor the change of the oil composition. On the matrice exhaust owing to a SFE at 9.0 MPa, one further extraction at 323.15 K and 24.0 MPa was performed to obtain a mixture of compounds with higher molecular weight. Finally, the antibacterial and antifungal activity of the various extracts has been assayed.

Antifungal activity of essential oils extracted from Agastache mexicana ssp. xolocotziana and Porophyllum linaria against post-harvest pathogens

“…1). In the case of methyl eugenol, it has been also identified as a major component in the essential oils of other plants, such as Piper divaricatum (Da Silva et al, 2010), Croton nepetaefoilus (Fontenelle et al, 2008), Laurus nobilis (Marzouki et al, 2009), and Artemisia dracunculus (Meepagala et al, 2002). Estragole has also been reported as a major compound in extracts of various Croton species (Donati et al, 2014;Fontenelle et al, 2008), Foeniculum vulgare (Özcan et al, 2006), and Agastache rugosa (Shin, 2004).…”

Effect of Laurus nobilis extract on the functioning of liver against ccl4 induced toxicity

“…The presence of the high contents of eugenol, methyl eugenol and fatty acid methyl esters together with other active components was reported (Marzouki et al, 2009) and it could contribute to its overall antioxidative and antibacterial activity (Croxen & Finlay, 2010) are also known to promote the wound-healing process (Ultee et al, 2002;Vardapetyan et al 2014). Along with the normalization of the liver to the 20th day there is a loosening of liver tissue ( Figure.1 (C)) and signs of expressed lipidation, accompanied by a decrease in the level of urea, albumin, cholesterol and TAG below those of the intact group that sertify possibility of liver damage in transition chronic form.…”

Seasonal and Geographical Variation of Laurus nobilis L. Essential Oil from Tunisia~!2009-05-04~!2009-09-10~!2009-12-30~!

“…Moreover, pharmacological activity, which includes antifungal, anti-diabetic, and antiinflammatory activities [6], have been also demonstrated. The chemical composition of the leaf essential oil, obtained from different methods of isolation, were extensively studied by many researchers [8][9][10][11][12]. Generally, the yield and composition of the oil varies, depending upon the origin, the collection period, and the growth stage of the plant [13].…”