Biological activities of the sulfated polysaccharide from the vascular plant Halodule wrightii

Abstract

Abstract:A sulfated polysaccharide (SPSG) was successfully isolated from seagrass Halodule wrightii Asch., Cymodoceaceae, and its antioxidant and anticoagulant activities were investigated. The data presented here showed that the SPSG is a 11 kDa sulfated heterogalactan with a sulfatation degree of 20.63% and it also contains glucose and xylose. SPSG antioxidant activities were evaluated using several in vitro assays and the anticoagulant activity was evaluated by aPTT and PT tests. These assays suggested that the SPSG possessed remarkable antioxidant properties in different in vitro assays and an outstanding anticoagulant activity 2.5-fold higher than that of heparin Clexane ® in the aPTT test. This data represents the first reported on the sulfated polysaccharide biological activities from seagrass. These results indicate that SPSG can be considered in the future as a drug utilized in treating diseases from these systems.

Microwave-assisted extraction and pharmacological evaluation of polysaccharides from Posidonia oceanica

“…The results also indicate that galactose is the major monosaccharide component as detailed in Table 5. In comparison with other studies 21) , it was noted that some monosaccharides are commonly found in P. oceanica including glucose, galactose, xylose and the difference in the composition may be due to the extraction, the purification and the separation conditions or to environmental factors 22) . SEC/MALS/DRI was used to determine the distribution and range of molecular weight of PPO.…”

Extraction process optimization of sulfated galactan-rich fractions from Hypnea musciformis in order to obtain antioxidant, anticoagulant, or immunomodulatory polysaccharides

“…The antioxidant capacity assay is based on the reduction of molybdenum(VI) to molybdenum(V) by the sulfated galactan-rich fractions and the subsequent formation of a green phosphate/Mo(V) complex at acidic pH (Silva et al 2012). Tubes containing sulfated galactan-rich fractions and reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate, and 4 mM ammonium molybdate) were incubated at 95°C for 90 min.…”

Antinociceptive and Anti-Inflammatory Activities of Sargassum wightii and Halophila ovalis Sulfated Polysaccharides in Experimental Animal Models

“…The presence of sulfated polysaccharides was determined in three species, of which, the Ruppia maritima sulfated polysaccharide was purified and its structure was characterized. 19 To date, the sulfated polysaccharide of Halodule wrightii has been characterized for its antioxidant and anticoagulant activities. 19 We reported the existence of the anti-inflammatory activity in seagrass Halophila ovalis methanol extract.…”

“…19 To date, the sulfated polysaccharide of Halodule wrightii has been characterized for its antioxidant and anticoagulant activities. 19 We reported the existence of the anti-inflammatory activity in seagrass Halophila ovalis methanol extract. 20 However, there is no single report about the biological activities of sulfated polysaccharides from seagrass H. ovalis until now.…”