Biological Activities of Some Isoquinoline Alkaloids from Fumaria schleicheri Soy. Will.

Abstract

Fumaria schleicheri Soy. Will. is a species belonging to the Papaveraceae family, being widespread in East-Central and Southern Europe. As with numerous other species of the genus, it is used in traditional medicine for the treatment of hepatobiliary and digestive disorders. The aim of the present study consisted of the evaluation of its alkaloid content and the assessment of its in vitro antioxidant, anti-cholinesterase and cytotoxic potential. Total alkaloid content in the composition of the species was quantified by a spectrophotometrical method and they were individually identified and quantified by HPLC-DAD. The antioxidant capacity was investigated by the DPPH and FRAP methods, while the anti-cholinesterase activity was assessed by an adapted Ellman’s method. The in vitro cytotoxic activity was evaluated on BJ human fibroblasts and DLD-1 human colon adenocarcinoma cell lines. Results showed the presence of bicuculline, protopine, chelidonine, stylopine and sanguinarine, among which bicuculline, protopine, stylopine and sanguinarine were quantified, while the antioxidant and anti-cholinesterase assays showed valuable potentials. No cytotoxic effect was observed on BJ cell lines and selective cytotoxicity was expressed towards tumoral cells. In this context, F. schleicheri appears as an important medicinal species with significant potential of substitution with the officinal species.

Biological Activities and Chemical Profile of Gentiana asclepiadea and Inula helenium Ethanolic Extracts

“…The absorbance was determined at 500 nm, and TPA content was expressed as g CAE per 100 g of dry material. All the determinations were performed using a UV–V is spectrophotometer (Specord 200 Plus, Analytik Jena, Germany) [ 58 , 59 , 60 , 61 ].…”

“…The absorbance of obtained solutions was read at 450 nm, using Trolox as standard for the calibration curve (R2 = 0.992). The results of FRAP assay were expressed as μmol of Trolox Equivalents (TE) per 100 mL of extract [ 58 , 59 , 60 , 61 ]…”

“…The results of the DPPH assay were expressed as IC 50 value (μg/mL), representing the concentration of antioxidant capable of reducing the DPPH radical concentration by half [ 58 , 59 , 60 , 61 ].…”

“…Cell viability and proliferative capacity of treated cells were compared with the negative control. In addition, for each tested plant extract, the half-maximal inhibitory concentration (IC 50 ) value was calculated from the dose response curve obtained using non-linear regression [ 60 ].…”

Antiproliferative and Antimicrobial Effects of Rosmarinus officinalis L. Loaded Liposomes

“…The SK-Hep-1 (HTB-52 ™ ) cells were maintained in Minimum Essential Medium Eagle (MEM) supplemented with 10% fetal bovine serum and 1% Antibiotic-Antimycotic 100× and 1% L-glutamine in a 5% CO 2 incubator (Advantage-Lab, Schilde, Belgium) at 37 • C in a humidified atmosphere, according to standard conditions. The LX-2 cell line was maintained in Dulbecco's modified Eagle's medium (DMEM, high glucose) supplemented with 10% fetal bovine serum and 1% Antibiotic-Antimycotic 100× and 1% L-glutamine [52,53].…”