Biological activities of laminin

Kleinman, Hynda K.; Cannon, Frances B.; Laurie, Gordon W.; Hassell, John R.; Aumailley, Monique; Terranova, Victor P.; Martin, George R.; Dubois‐Dalcq, Monique

doi:10.1002/jcb.240270402

Cited by 425 publications

(173 citation statements)

References 59 publications

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“…Numerous physiological functions have been proposed for laminins (for review, see [32]), but the roles of different isoforms are still largely unknown. However, recent data on two inherited diseases involving laminin gene defects have shed some light on the function of two isoforms.…”

Section: Introductionmentioning

confidence: 99%

Primary structure and expression of a novel human laminin α4 chain

et al. 1995

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The complete primary structure of a novel human laminin a4 chain was derived from eDNA clones. The translation product contains a 24-residue signal peptide preceding the mature a4 chain of 1792 residues. The domain structure is similar to that of the recently described a3 chain [Ryan, Tizard, Van Devanter and Carter (1994) J. Biol. Chem. 269, 22779-227871. Northern analysis of RNA from human fetal and adult tissues revealed developmental regulation of expression. In adult, strong expression was observed in heart as well as lung, ovary, small and large intestines, placenta and liver, whereas weak or no expression was detected in skeletal muscle, kidney, pancreas, testis, prostate or brain. In contrast, fetal lung and kidney revealed high expression. In situ hybridization analysis of human fetal and newborn tissues showed expression of the laminin in or4 chain in certain mesenchymal cells in tissues such as smooth muscle and dermis.

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Section: Introductionmentioning

confidence: 99%

Primary structure and expression of a novel human laminin α4 chain

et al. 1995

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“…1,2 Structurally, laminin is a heterotrimeric protein comprising ␣, ␤ and ␥ subunits, of which several variants exist. 3 Thus, different subunit combinations result in a family of laminins.…”

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confidence: 99%

Laminin‐γ2 overexpression in head‐and‐neck squamous cell carcinoma

Patel

Aldridge

Ensley

et al. 2002

Intl Journal of Cancer

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To identify molecular markers for the progression of headand-neck squamous cell carcinoma (HNSCC), we used RNA arbitrarily primed (RAP) PCR to determine the qualitative and quantitative differences in gene expression between normal epithelial cells, those derived from dysplastic oral mucosa and invasive and metastatic HNSCC. Three differentially expressed DNA fragments (RAP20, RAP21, RAP26) that were upregulated in a tumor cell line (T45) were identified as being regions of the ␥2 subunit of human laminin-5. Northern blot analysis of total cellular RNA revealed overexpression of these transcripts in 6 of 7 HNSCC cell lines compared with normal epidermal keratinocytes. In contrast, no differences were observed in HeLa (cervical carcinoma) or HCT116 (colon carcinoma) cells. Laminin is a major component of the basement membrane, which performs multiple biologic roles, including cell attachment, spreading and migration as well as being involved in cellular proliferation and differentiation. 1,2 Structurally, laminin is a heterotrimeric protein comprising ␣, ␤ and ␥ subunits, of which several variants exist. 3 Thus, different subunit combinations result in a family of laminins. 3,4 Specific biologic activities of different laminins may be reflected in the fact that these molecules show tissue-specific expression. For example, laminin-5 is considered to be an epithelial laminin as it is found as a component of basement membranes in epithelial tissues but not in mesenchyme. 5,6 Furthermore, cytokines that regulate epithelial homeostasis, such as transforming growth factor (TGF)-␤, modulate the expression of laminin-5 polypeptides in epidermal keratinocytes. 7 A role for laminin isoforms in tumor cell invasion is becoming apparent. 8 For instance, attachment of cancer cells is mediated in part by laminins. 9,10 Additionally, increased cell-surface expression of laminin was found in highly metastatic fibrosarcoma cells compared to cells of low metastatic potential. 11 Furthermore, expression and activity of matrix metalloproteases, which play critical roles in cellular invasion, are elevated in the presence of laminin, 12 and metastasis of melanoma cells is also enhanced. 13 Further studies have demonstrated that attachment and metastasis of tumor cells can be inhibited by incubation with antilaminin antibodies 14,15 or synthetic laminin peptides. 16 Several reports have implicated laminin-5 in epithelial tumor cell invasion. In one study, overexpression of the ␥2 chain of laminin-5 was found in 26 of 27 carcinomas, while various mesenchymal tumors did not express this molecule. 17 Furthermore, the highest expression of ␥2 occurred at areas of active invasion in colon adenocarcinomas, and a clear correlation was found with tumor budding. 17 In a subsequent study, these findings were extended to examine expression in a larger series of human cancers, 18 demonstrating that levels of ␥2 were indeed the highest in squamous carcinomas, particularly in cells adjacent to surrounding stromal tissue and in deeply invading cell ne...

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“…Laminin probably acts as a growth stimulating fator of tumor cells and promotes their invasion and metastasis by the adhesion to type IV collagen or the secretion of type IV collagenase (Kleinman et al 1985). In our irradiation group the changes in the laminin stain of giant tumor cells can be attributed to one of several causes.…”

Section: Discussionmentioning

confidence: 70%

The irradiation effects on the cytoskeletons of C3H/He mouse mammary tumor cells and vascular basement membrane in relation to vascular invasion: A model of intraoperative radiotherapy.

Kobayashi

1988

Tohoku J. Exp. Med.

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We investigated the short-term effects of a single high-dose radiation upon transplanted MM46 tumor cells in mice by means of immunohistochemistry and electron microscopy. The irradiation induced : 1) giant cell formation from the 3rd day, 2) arrest of tumor cell mitosis in prophase and metaphase due to the disorganization of the mitotic spindles, 3) changes in immunoreactivity of laminin and cytoskeletons, and 4) multilayering of the vascular basal lamina and perivascular fibrosis. The above findings suggest a decrease in tumor cell compliance, growth and invasiveness and the potentiation of defensive host responses against vascular invasion after irradiation. The analysis of the temporal sequences of the events indicates that the time lapse between the optimal host response, tumor growth and invasion constitutes a critical period.tumor ; intraoperative radiotherapy ; vascular invasion ; cytoskeleton ; laminin Intraoperative radiotherapy (TORT) is designed to sterilize the cancer cells that evade a surgical resection by irradiation with a single high-dose of electron beams. The morphologic changes have been studied in various tissues after such

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Biological activities of laminin

Cited by 425 publications

References 59 publications

Primary structure and expression of a novel human laminin α4 chain

Primary structure and expression of a novel human laminin α4 chain

Laminin‐γ2 overexpression in head‐and‐neck squamous cell carcinoma

The irradiation effects on the cytoskeletons of C3H/He mouse mammary tumor cells and vascular basement membrane in relation to vascular invasion: A model of intraoperative radiotherapy.

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