Biological activities of human interferon and 2??5? oligoadenylates in plants

Кулаева, О. Н.; Fedina, A. B.; Burkhanova, E. A.; Karavaiko, Natalya N.; Karpeisky, M.Ya.; Kaplan, Igor B.; Taliansky, Michael; Atabekov, J.G.

doi:10.1007/bf00040598

Cited by 17 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are a few reports on animal cytokine expression in plants, but no definitive effect of the expressed hIFN on the plants has been reported (de Zoeten et al 1989;Kulaeva et al 1992;Mori et al 1993;Rudas et al 1997). The attenuation of symptoms observed in plants inoculated with pClYVV/hIFN/P1 is not due to the expression of hIFN, as plants inoculated with pClYVV/hIFN/CP did not display the same attenuative effect.…”

Section: Discussionmentioning

confidence: 92%

Positional effect of gene insertion on genetic stability of a clover yellow vein virus-based expression vector

et al. 2003

View full text Add to dashboard Cite

The stability of the inserted genes in the viral expression vector varied depending on the sequence introduced and the position of insertion. Infectious cDNA to Clover yellow vein virus (pClYVV) was modified to insert a foreign gene at two independent sites: one, along with a polylinker, between the NIb and CP genes (pClYVV/CP/ W) and the other between P1 and HC-Pro (pClYVV-Pst/ CP). The green fluorescent protein (GFP) gene was inserted into either pClYVV/CP/W or pClYVV-Pst/CP. GFP gene was stably maintained and expressed in both vectors following serial passages in plants. Progeny viruses from both constructs accumulated in similar amounts and at rates of 70%-80% of that of the wild-type virus. On the other hand, progeny viruses carrying the human interferon-α (hIFN) gene cloned in pClYVV-Pst/CP were genetically unstable owing to frequent deletions of the cloned gene during passage through plants. In contrast, the hIFN sequence cloned in pClYVV/CP/W was stably maintained in viruses after several passages in broad bean plants, and the progeny virus accumulated at the rate of about 50%-100% of that of the wild-type virus. The nucleotide sequence analyses indicated that the genetic instability of the inserted sequence results from homologous recombination of viral vector and inserted DNA sequences; it is not due to the inserted sequence alone.

show abstract

Section: Discussionmentioning

confidence: 92%

Positional effect of gene insertion on genetic stability of a clover yellow vein virus-based expression vector

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Indeed, the use of interferon and 2-5A in optimal and relatively low concentrations resulted in a few times increase of cytokinins in tobacco leaves. However, a further increase of interferon concentration had an opposite effect (Kulaeva et al, 1992).…”

Section: Discussionmentioning

confidence: 97%

Effect of some immunomodulators and zeatin on susceptibility of wheat to powdery mildew

Бабоша¹

2019

Turk J Bot

View full text Add to dashboard Cite

The effects of zeatin, salicylic acid, and plant oligoadenylates on susceptibility of wheat seedlings to powdery mildew and the shape of dose-response curves for immunomodulation were studied. Salicylic acid and zeatin produced complex dose-responses with upregulation or downregulation of plant susceptibility. Under different experimental conditions, the dose-response curve for zeatin varied in shape from a curve with a maximum to a curve with a minimum of susceptibility to powdery mildew. More complicated curves with a resistance region framed with regions of increased susceptibility were also found. Plant oligoadenylates inhibited the development of powdery mildew at optimal concentrations. The increasing concentrations of salicylic acid and plant oligoadenylates, when combined with zeatin, led to a gradual change in the shape of the zeatin concentration curve. Treatment with salicylic acid and plant oligoadenylates in these experiments may simulate plant responses under biotic and abiotic stresses. Thus, exogenous zeatin was applied to model changes in the phytohormone metabolism. The complex multiphase nature of dose-response is proposed to cause a kind of random-number generator that produces variability in the physiological and immunological status of plants in natural conditions. Variation in susceptibility of individual plants may be crucial for the survival of infected plants and the stabilization of plant-pathogen interactions.

show abstract

“…Several lines of evidence suggest that some components of this anti-viral pathway may operate in plants : (i) tobacco plants that have been challenged with TMV have been shown to contain a double-stranded RNA-dependent ATP polymerizing activity, (ii) plant extracts can synthesize oligoadenylate-like compounds possessing anti-viral activity in vitro and (iii) exogenous application of 2'-5' oligoadenylates and human interferon to leaves of tobacco and wheat plants causes induction of PR proteins and increased cytokinin activity (Kulaeva et al ., 1992) . Taken together, this suggests that expression of a mammalian oligoadenylate synthetase cDNA might confer non-specific antiviral activity in transgenic plants .…”

Section: Mammalian Oligoadenylate Synthetasementioning

confidence: 99%

Strategies for engineering virus resistance in transgenic plants

Kavanagh

Spillane

1995

Euphytica

View full text Add to dashboard Cite

Transgenic virus-resistant plants were first produced in 1986 by genetically engineering tobacco plants to express the coat protein of tobacco mosaic virus . The introduction of coat protein transgenes has since proved to be an extremely effective and generally applicable approach to engineering virus resistance in crop plants . Extensive field trials with transgenic, virus-resistant tobacco, tomato, potato and cucumber lines have confirmed not only the durability of the resistance under natural conditions but the ease with which virus-resistant lines retaining the original cultivar traits can be recovered .A number of alternative anti-viral strategies based on transgenes from a surprisingly wide variety of sources have also been developed . These include the use of viral genes coding for proteins involved in the replication cycle and in systemic transport of viruses within the plant, the use of interfering viral RNA sequences, and the use of transgenes derived from plant and animal sources . In the latter category, the use of mammalian antibodies to confer disease resistance in plants is a particularly exciting new development . Considerable progress has also been made towards the molecular cloning of natural anti-viral resistance genes in plants .

show abstract

Biological activities of human interferon and 2??5? oligoadenylates in plants

Cited by 17 publications

References 35 publications

Positional effect of gene insertion on genetic stability of a clover yellow vein virus-based expression vector

Positional effect of gene insertion on genetic stability of a clover yellow vein virus-based expression vector

Effect of some immunomodulators and zeatin on susceptibility of wheat to powdery mildew

Strategies for engineering virus resistance in transgenic plants

Contact Info

Product

Resources

About