Biological Activities and Phytochemical Screening of Ethanol Extracts From Adonis Paryadrica (Ranunculaceae)

Abstract

In this study, secondary metabolite profiles of flower, leaf and root of Adonis paryadrica ethanolic extracts were performed by using a newly developed and validated LC-MS/MS method. For this purpose, the LC-MS/MS system was used for the quantitative and qualitative analysis of 37 phytochemicals. The main component of the flower, leaf and root extracts was the quinic acid (160, 111 and 64 mg/kg, respectively). Antioxidant activities were determined using methods such as DPPH (SC50, mg/mL), FRAP (µM TEAC), CUPRAC (µM TEAC), ABTS (SC50, mg/mL) and TPC (GAE, µg/mL). While the highest antioxidant activity value for FRAP, CUPRAC, ABTS and TPC methods was determined in the leaf part of the plant, this value was determined in the flower part for DPPH. The antibacterial effects of the plant extracts were determined by liquid microdilution and agar diffusion methods. As a result, antimicrobial effect of flowers, leaf and all sections of plant extracts were determined except plant root extracts which did not display any activity against bacteria. Antimicrobial activity of the plant flower extract was found to be higher especially against to P. vulgaris with 3.5 mg/mL MIC. Rezumat În acest studiu, au fost evaluați metaboliții secundari din extractele etanolice provenite de la florile, frunzele și rădăcinile de Adonis paryadrica, utilizând o metodă LC-MS/MS. În acest scop, s-au pus în evidență 37 fitocompuși, componenta principală fiind acidul quinic (160, 111 și respectiv 64 mg/kg). Activitatea antioxidantă a extractelor a fost determinată prin metode precum DPPH, FRAP, CUPRAC, ABTS și TPC. Extractul de frunze a prezentat activitatea antioxidantă cea mai pronunțată prin metodele FRAP, CUPRAC, ABTS și TPC, iar extractul alcoolic de flori prin metoda DPPH. Efectele antibacteriene ale extractelor au fost determinate prin metoda microdiluției și cea difuzimetrică. Cu excepția extractului de rădăcină, toate celelalte au prezentat activitate antibacteriană. Cea mai mare valoare a CMI pentru P. vulgaris a fost obținută pentru extractul de flori.

Antioxidant and antimicrobial activities of methanol extracts from Adonis paryadrica (Asteraceae) – a critically endangered endemic species growing in the Turkish flora

“…Antioxidant studies of species belonging to the genus Adonis are limited in the literature (Mohadjerani et al, 2014;Ucuncu et al, 2020;Guo et al, 2022). In antioxidant studies of A. paryadrica with only ethanol extracts, researchers used different parts of the plant and obtained effective results on the gallic acid equivalent (Ucuncu et al, 2020). The data obtained from this study also showed that the difference in the extract had an effect on the antioxidant properties of the Trolox equivalent.…”

“…Evaluation of the biological activities of valuable natural metabolites of plants is one of the most important research areas today and there are many studies carried out in this field (Cüce et al, 2017(Cüce et al, , 2019Cüce and Basançelebi, 2021;Sarikurkcu et al, 2021). The different techniques and methods used in these studies or the different extraction techniques and solvents for the same species can be shown among the reasons for the results obtained from the studies (Jahanban-Esfahlan et al, 2019;Ucuncu et al, 2020;Sarikurkcu et al, 2020). Due to this situation, the researchers preferred ethanol extracts for A. paryadrica in previous studies and reported that they obtained similar results in terms of total phenolic contents with the data in our study (Ucuncu et al, 2020).…”

“…The different techniques and methods used in these studies or the different extraction techniques and solvents for the same species can be shown among the reasons for the results obtained from the studies (Jahanban-Esfahlan et al, 2019;Ucuncu et al, 2020;Sarikurkcu et al, 2020). Due to this situation, the researchers preferred ethanol extracts for A. paryadrica in previous studies and reported that they obtained similar results in terms of total phenolic contents with the data in our study (Ucuncu et al, 2020). The strength of this study is that the percentage yield and total flavonoid content values were not given in the previous study on A. paryadrica.…”

“…On the other hand, in terms of FRAP reducing power activity, root extracts were followed by leaf with 42.11 mg/ml and flower (34.17 mg/ml) extracts, respectively (Table 3). Antioxidant studies of species belonging to the genus Adonis are limited in the literature (Mohadjerani et al, 2014;Ucuncu et al, 2020;Guo et al, 2022). In antioxidant studies of A. paryadrica with only ethanol extracts, researchers used different parts of the plant and obtained effective results on the gallic acid equivalent (Ucuncu et al, 2020).…”

“…There are a limited number of studies on phytochemical components of this genus in the literature (Mohadjerani et al, 2014;Kuroda et al, 2018;Ucuncu et al, 2020). Although a study has been reported on the phytochemical and biological activity of ethanol extracts of A. paryadrica, however, there is no comprehensive study in the literature on the biological activities of extracts obtained with other solvents up to the best of our knowledge.…”