Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga

Araújo, Marlon Heggdorne de; Silva, Isabel C V da; Oliveira, Pollyana Felix de; Barreto, Arielly R.R.; Konno, Tatiana Ungaretti Paleo; Esteves, Francisco de Assis; Barth, Thiago; Aguiar, Fernando Nalesso; Lopes, Norberto P.; Dermenjian, Renee K.; Guimarães, Denise O.; Leal, Ivana Corrêa Ramos; Lassounskaia, Elena; Muzitano, Michelle Frazão

doi:10.1016/j.bjp.2017.07.005

Cited by 16 publications

(9 citation statements)

References 44 publications

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“…De Araujo et al [61] evaluated the crude hydroalcoholic extract and fractions from leaves of P. mucronata from Rio de Janeiro (Brazil) using in vitro antioxidant activity assay of DPPH radical. Results of this study showed value EC 50 =96.05 µg/ml for and these results correlated with total phenols (9.5 mg EAG/g, 5.5 mg EAG/g, 4.9 mg EAG/g).…”

Section: Resultsmentioning

confidence: 99%

“…sublanceolata, P. nitida, P. tenuifila, P. coriacea, P. foetida [50], extracts of peel of P. edulis [51,52], of seeds of P. edulis [53], of bark (exocarpium and mesocarpium) of P. edulis [54], various preparations of P. incarnata [55][56][57], fruit juices from seven passion fruit (Passiflora spp.) cultivars: P. edulis cultivars Purple, Frederick, Yellow, Pink, P. edulis f. flavicarpa, P. maliformis and P. quadrangularis [58], aqueous extract from leaf of P. edulis [59,60], ethanolic and fractionated extract from leaf of P. mucronata [61], fractionated extracts from leaves and stems of P. quadrangularis and P. maliformis [62], of P. alata [63], hydroethanol leaf extract of P. nitida [64], acetone extract of P. subpeltata leaves [65]. However, it should be emphasized that results of these studies are different not only due to various solvents used in conventional extraction but also by different in vitro and in vivo methods used to estimate the antioxidant activity and by different species, part of plants, chemical composition and environmental factors influencing various horticultural crops of Passiflora in countries around the world.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of in vitro antioxidative activities of crude methanolic extracts of three species of Passiflora from greenhouse using DPPH, ABTS and FRAP methods

et al. 2019

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Summary Introduction:. It is well documented that many species from Passifloraceae family can provide edible and nutritious fruits while the leaves of cultivated plants are renewable and waste material. This biomass may be further used in various sectors, especially as a bioactive food additive and as source of innovative pharmaceuticals, cosmetics or feed additives. The biomaterials and green chemistry are new sectors bioeconomy according to the high-level horizontal strategies and bio-based industries in Europe. In recent years, attention has been paid to the biological activity and phytochemical profiles of extracts from different species of Passiflora. However, there is little comparative studies using the same procedures and techniques in the same laboratory conditions for study of plant material obtained from the similar greenhouse conditions. Objective: This study was focused on the examination of antioxidative activities of low concentrations of crude extracts from leaves of Passiflora incarnata L., Passiflora caerulea L., and Passiflora alata Curtis. Methods: The activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging and ferric reducing antioxidant power (FRAP) methods. Results of study were supported by estimation of chemical composition with secondary metabolites profiling in extracts which were carried out previously for the same extracts from three Passiflora species. One-way ANOVA analysis revealed significant differences in the antioxidant activity of various concentrations of the extracts using the DPPH and ABTS radical models, and FRAP method. Results: Measurement of antioxidant capacity (expressed as trolox equivalent, TE) showed that the most active was extract of P. caerulea > P. alata > P. incarnata. Phytochemical analysis for extracts of P. caerulea and P. incarnata showed greater similarities in metabolites content than P. alata. However, comparative statistical analysis of antioxidant activity showed that despite this phytochemical similarities, extract from P. alata leaves had higher activities than extract from leaves P. incarnata. Antioxidant effect of extract from P. alata can be explain by terpenoids presented in this extract. In this work, there have been discussed activities against Acanthamoeba castellanii strain, antibacterial and antifungal activities against selected clinical microorganisms (Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, and Candida albicans, Micro-sporum gypseum), and anti-leukemic activities tested in human acute lymphoblastic leukemia cell lines for this extracts, which have been described in previous authors’ publications. Conclusion: Our current and previous studies showed that the same crude extracts from leaves of P. alata, P. caerulea, P. incarnata exerted not only antioxidant potential in vitro but also few interesting properties such as antibacterial, antifungal, amoebostatic, amoebicidal activities, which indicate the possibility of using these extracts in both a healthy diet and natural cosmetics. Leaves of this species may become an interesting source of biomaterials which can exert health-promoting effects.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of in vitro antioxidative activities of crude methanolic extracts of three species of Passiflora from greenhouse using DPPH, ABTS and FRAP methods

et al. 2019

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“…The plates were sealed and incubated at 37 °C and 5% of carbon dioxide (CO 2 ) for 7 days for M. bovis BCG or 5 days for M. tuberculosis strains. The mycobacterial cultures were incubated after these periods for 3 h with MTT solution (5 mg/mL) and then treated overnight with lysis buffer (20% w/v Sodium dodecyl sulfate (SDS)/50% dimethylformamide (DMF) in distilled water -pH 4.7) (Gomez-Flores et al 1995 for the experiments and incubated for 24 h. Cells were stimulated after this period with 1 µg/mL LPS (Escherichia coli 0111:B4; Sigma-Aldrich) and treated with plant samples at concentrations of 100, 20, 4 and 0.8 μg/mL for 24 h. Next, culture supernatants were collected for NO and TNF-α assays (Araujo et al 2017).…”

Section: Anti-mycobacterial Activitymentioning

confidence: 99%

Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosis

et al. 2021

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Tuberculosis (TB) still constitutes a threat to public health in various regions of the world. The existing treatment is long and has many side effects. The need to identify new anti-TB compounds and also adjuvants to control exacerbated inflammation in severe TB cases is relevant. Therefore, the objective of this study was to evaluate the anti-mycobacterial activity of extracts and fractions in vitro from plant species collected in the Restinga of Jurubatiba, in Rio de Janeiro state, Brazil. In addition, to verify their immunomodulatory action and cytotoxicity on macrophages. The dichloromethane fraction of Kielmeyera membranacea and Eremanthus crotonoides showed the lowest MIC50 against Mycobacterium bovis BCG (0.95 ± 1.08 and 2.17 ± 1.11 μg/mL, respectively) and M. tuberculosis H37Rv (4.38 ± 1.19 and 15.28 ± 1.21 μg/mL, respectively). They were also able to inhibit the NO and TNF-α production in LPS-stimulated macrophages, without being toxic to cells. Using gas chromatography analysis coupled with mass spectrometer it was possible to suggest the presence of fatty acids and terpenes in the most promising fractions. Those compounds have been described for their anti-mycobacterial activity. These results have enabled identifying Kielmeyera membranacea and Eremanthus crotonoides as the most promising studied species in searching for new anti-TB compounds with dual activity.

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“…In general, for protection against mycobacteria, the production of proinflammatory mediators by the infected macrophages is essential. However, in cases of severe forms of TB, additional anti-inflammatory therapy is required to prevent excessive inflammation [6,7].…”

Section: Introductionmentioning

confidence: 99%

Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Triterpenes and Alkaloids from Psychotria nuda (Cham. & Schltdl.) Wawra

et al. 2019

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A phytochemical study of leaves and twigs of Psychotria nuda resulted in 19 compounds, including five indole alkaloids, N,N,N-trimethyltryptamine, lyaloside, strictosamide, strictosidine, and 5α-carboxystrictosidine; two flavonolignans, cinchonain Ia and cinchonain Ib; an iridoid, roseoside; a sugar, lawsofructose; a coumarin, scopoletin; a diterpene, phytol; three triterpenes, pomolic acid, spinosic acid, and rotungenic acid; and five steroids, sitosterol, stigmasterol, campesterol, β-sitosterol-3-O-β-d-glucoside, and β-stigmasterol-3-O-β-d-glucoside. Some compounds were evaluated for their in vitro activity against Mycobacterium tuberculosis and their ability to inhibit NO production by macrophages stimulated by lipopolysaccharide (LPS). The compounds pomolic acid, spinosic acid, strictosidine, and 5α-carboxystrictosidine displayed antimycobacterial activity with minimum inhibitory concentrations ranging from 7.1 to 19.2 µg/mL. These compounds showed promising inhibitory activity against NO production (IC50 3.22 to 25.5 μg/mL). 5α-carboxystrictosidine did not show cytotoxicity against macrophages RAW264.7 up to a concentration of 100 µg/mL. With the exception of strictosamide, this is the first report of the occurrence of these substances in P. nuda.

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Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga

Cited by 16 publications

References 44 publications

Comparison of in vitro antioxidative activities of crude methanolic extracts of three species of Passiflora from greenhouse using DPPH, ABTS and FRAP methods

Comparison of in vitro antioxidative activities of crude methanolic extracts of three species of Passiflora from greenhouse using DPPH, ABTS and FRAP methods

Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosis

Antimycobacterial and Nitric Oxide Production Inhibitory Activities of Triterpenes and Alkaloids from Psychotria nuda (Cham. & Schltdl.) Wawra

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