Biological activities and phenolic compounds of olive oil mill wastewater from Abani, endemic Algerian variety

Abstract

The current study aimed to determination of cytotoxicity, antioxidant, anti-inflammatory, anti-hemolytic, and anticoagulant activities of phenolic compounds extracted from olive oil mill wastewater (OMW) issue from the cold extraction of olive oil from Khenchela eastern in Algeria. The LC–MS (liquid chromatography–mass spectrometry) results were revealed the presence of 20 phenolic compounds in the extract of OMW and mostly consisted of Kaempferol, 4,5-di-O-caffeoyquinic acid, quinic acid, and caffeic acid. The extracts possessed effective reducing power (FRAP) and high radical scavenging activity against DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS + (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) free radicals, and it inhibited cytochrome c reduction in a dose-dependent manner. They exert a protective effect on red blood cells, and they were found to exhibit the highest inhibitory effect anti-inflammatory activity using inhibition of protein denaturation (IPD) and membrane stabilizing potential (MSP) tests (80.46 ± 3.81 µg/mL and 87.43 ± 0.66 µg/mL) more than the standard used. The extract also showed the greatest anticoagulant activity in both the endogenous and exogenous routes (44.77 ± 0.25 s and 15.84 ± 0.12 s, respectively). Based on these findings, it is reasonable to infer that OMW is a good source of natural phenolic compounds with potential antioxidant, anti-inflammatory, and anticoagulant properties.

In vitro and in silico investigation of garlic’s (Allium sativum) bioactivity against 15-lipoxygenase mediated inflammopathies

“…Proton loss and free radical neutralization are the key mechanisms through which phenolic substances exhibit antioxidant effects (47). Moreover, it has been reported that the polyphenolic metabolites derived from plants also exhibit high antiinflammatory as well as anticoagulant properties (48). In this study, the aqueous extract possessed a significant concentration of phenols and flavonoids, indicating the strong antioxidant activity of garlic.…”

Impact of Storage Duration on Polyphenolic Composition, Biological Activities, and Biodegradation of Olive Mill Wastewater

“…The comparison of the IC50 values for the extracts with those of the standard antioxidants (Ascorbic acid, Trolox, and BHT) allows for the evaluation of the extracts' antioxidant potential relative to well-known synthetic antioxidants. A lower IC50 value suggests that the extracts have strong antioxidant capabilities and are effective in neutralizing free radicals (Gueboudji et al, 2022a).…”

“…It was measured according to the method described by (Gueboudji et al, 2022a) with some adjustment. In this study, blood was collected from a healthy volunteer who had not taken non-steroidal anti-inflammatory drugs (NSAIDs) for two weeks.…”

Identification and Pharmacological Activities of Secondary Metabolites of Phytochemicals

“…The biological activities of phenolic compounds are exploited in pharmaceutical industries because of their antiinflammatory, antioxidant, anti-hypertensive, anti-microbial, hepatoprotective properties, antidiabetic, and anticancer, etc. [11]. Arctigenin, arctiin and trachelogenin have been reported as anti-HIV, anticancer [12][13].…”