Biological activities and luteolin derivatives of Verbascum eskisehirensis Karavel., Ocak   Ekici

Öztürk, Gözde; Ağalar, Hale Gamze; Yıldız, Gülsüm; Göğer, Fatih; Kırımer, N.

doi:10.12991/jrp.2019.160

Cited by 4 publications

(4 citation statements)

References 30 publications

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“…These results could provide insights into the key catalytic residues and the catalytic mechanisms of O-GTs from liverworts. Such mutant enzymes might prove useful in the biocatalytic synthesis of specific luteolin glucosides, due to the position of conjugation could affect both the bioavailability and health beneficial activity of luteolin for humans (Aziz et al, 2018;Baskar et al, 2011;Öztürk et al, 2019). , 6, 12, 24, 36, 48, 60, and 72 h).…”

Section: Crucial Amino Acid Residues Involved In the Regiospecificity...mentioning

confidence: 99%

Functional specialization of two UDP‐glycosyltransferases MpUGT735A2 and MpUGT743A1 in the liverworts Marchantia polymorpha

Zhu,

Ta,

et al. 2023

Journal Cellular Physiology

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Family 1 UDP‐glycosyltransferases (UGTs) are known to glycosylate multiple secondary plant metabolites and have been extensively studied. The increased availability of plant genome resources allows the identification of wide gene families, both functional and organizational. In this investigation, two MpUGT isoforms were cloned and functionally characterized from liverworts marchantia polymorpha and had high glycosylation activity against several flavonoids. MpUGT735A2 protein, in particular, tolerates a wide spectrum of substrates (flavonols, flavanones, flavones, stilbenes, bibenzyls, dihydrochalcone, phenylpropanoids, xanthones, and isoflavones). Overexpression of MpUGT735A2 and MpUGT743A1 in Arabidopsis thaliana enhances the accumulation of 3‐O‐glycosylated flavonol (kaempferol 3‐O‐glucoside‐7‐O‐rhamnose), consistent with its in vitro enzymatic activity. Docking and mutagenesis techniques were applied to identify the structural and functional properties of MpUGT735A2 with promiscuous substrates. Mutation of Pro87 to Ser, or Gln88 to Val, substantially altered the regioselectivity for luteolin glycosylation, predominantly from the 3′‐O‐ to the 7‐O‐position. The results were elucidated by focusing on the novel biocatalysts designed for producing therapeutic flavonoids. This investigation provides an approach to modulate MpUGT735A2 as a candidate gene for diverse glycosylation catalysis and a tool to design GTs with new substrate specificities for biomedical applications.

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Section: Crucial Amino Acid Residues Involved In the Regiospecificity...mentioning

confidence: 99%

Functional specialization of two UDP‐glycosyltransferases MpUGT735A2 and MpUGT743A1 in the liverworts Marchantia polymorpha

Zhu,

Ta,

et al. 2023

Journal Cellular Physiology

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“…TEAC assay was performed as in our previous Verbascum publication [26]. This assay assesses the capacity of a compound to scavenge the stable ABTS radical in comparison tothe antioxidant activity of Trolox, a water-soluble form of vitamin E that is used as a standard.…”

Section: Trolox Equivalent Antioxidant Capacity (Teac Assay)mentioning

confidence: 99%

“…LC-MS/MS analyses were carried out using a similar method as the previous study (26), with a slight modification, using Methanol as the solvent in gradient elution.…”

Section: Lc/ms Profilesmentioning

confidence: 99%

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Determination of cytotoxic, antioxidant activities and LC/MS-MS profiles of three endemic Verbascum L. species

Küçük,

Has,

Göger

et al. 2023

Eur J Life Sci

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The Verbascum genus includes many species used in folk medicine or the treatment of various diseases. In this study, the cytotoxic, antioxidant activity, and LC/MS-MS profiles of three Verbascum species, which are endemic in Eskişehir and its surroundings, were investigated. The cytotoxic effects of methanol extract of V.detersile, V. eskisehirensis, and V.gypsicola species on the cervical (HeLa) and ovarian cancer (SKOV-3) cells were investigated using a colorimetric assay. The results indicated that cytotoxic effect was not observed after treatment of SKOV-3 cells with Verbascum. On the other hand, the cytotoxic activity of V. detersile was found to be 0.1910 mg/dL and 1.057 mg/dL for HeLa cells after 24 or 48 hours incubation with V.detersile, respectively. The antioxidant activity was determined as 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical scavenging activity, trolox equivalent antioxidant capacity (TEAC assay), and also the total phenolic content of the samples was found. Total phenols were estimated as Gallic acid equivalents (GAE), expressed as mg Gallic acid/in 1g extract previously described by Singletton. LC/MS profiles separation and detection of phytochemicals of the extract were performed on a Shimadzu UPLC system consisting of a vacuum degasser, an autosampler (SIL20A Shimadzu Autosampler), a binary pump (LC20AD Shimadzu), an oven (CTO20A Shimadzu Column Oven) and DAD dedector (SPD M20A Shimadzu DAD Detector). In terms of antioxidant activity, V. gypsicola was found to have the least antioxidant activity among the three extracts, which is also correlated with the total amount of phenolic content in its content. In this way, it differs from other species. V. detersile exhibits a different chemical profile from the other two species with the iridoid catalpol derivatives it contains. Apigenin pentoside is the only flavonoid molecule detected in V. detersille.

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Antimicrobial and antibiofilm studies on three endemic species of Verbascum L. (Scrophulariaceae) in Türkiye

Soyer,

Küçük,

Tunalı

2024

Int. J. Sec. Metabolite

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The consumption of traditional medicinal herbs has gained popularity as a viable alternative approach for addressing microbial infections or infectious structures. In this study, Verbascum detersile, Verbascum eskisehirensis, and Verbascum gypsicola endemic species belonging to the Scrophulariaceae family, spreading in Eskişehir and its surroundings, were collected from natural habitats and evaluated in terms of antimicrobial and antibiofilm activities. In biological activity studies, different concentrations of three plant extracts showed various antimicrobial and antibiofilm activities on selected standard microorganism cultures (Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 14990, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 90028, and Candida krusei ATCC 6258). It was observed that the selected three endemic Verbascum extracts had a minimum inhibitory and minimum biofilm eradication concentration value of 1250 μg/mL.

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Biological activities and luteolin derivatives of Verbascum eskisehirensis Karavel., Ocak Ekici

Cited by 4 publications

References 30 publications

Functional specialization of two UDP‐glycosyltransferases MpUGT735A2 and MpUGT743A1 in the liverworts Marchantia polymorpha

Functional specialization of two UDP‐glycosyltransferases MpUGT735A2 and MpUGT743A1 in the liverworts Marchantia polymorpha

Determination of cytotoxic, antioxidant activities and LC/MS-MS profiles of three endemic Verbascum L. species

Antimicrobial and antibiofilm studies on three endemic species of Verbascum L. (Scrophulariaceae) in Türkiye

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