Biological 31P NMR Spectroscopy

O'Neill, I.K.; Richards, C. P.

doi:10.1016/s0066-4103(08)60394-4

Cited by 27 publications

(6 citation statements)

References 308 publications

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“…Verification of the 0-CH3 location in GDP/3me and GTP7me and in the corresponding 7-methylated derivatives was based mainly on 31P NMR spectroscopy. The chemical shifts of Pa, P^, and P7 in GDP and GTP were assigned according to published work (Labotka et al, 1976; O'Neil & Richards, 1980) (Table IV). The Pa signal had a chemical shift typical of Pa in nucleoside triphosphates; without decoupling of protons, the signals were doublets that broadened to two triplets because of coupling with protons 5' and 5"; 7(P,H) = 5-6 Hz and /(P0,P") = 18-22 Hz.…”

Section: Resultsmentioning

confidence: 99%

Chemical synthesis and characterization of 7-methylguanosine cap analogs

Darżynkiewicz¹,

Ekiel²,

Tahara³

et al. 1985

Biochemistry

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Section: Resultsmentioning

confidence: 99%

Chemical synthesis and characterization of 7-methylguanosine cap analogs

Darżynkiewicz¹,

Ekiel²,

Tahara³

et al. 1985

Biochemistry

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“…Dimyristoyl-0006-2960/81 /0420-5576S01.25/0 © 1981 American Chemical Society phosphatidylcholine (DMPC)1 exhibits a gel to liquid-crystalline transition temperature, convenient for membrane enzyme reconstitution studies. However, most physical characterizations of DMPC dispersions in water have been performed with multilamellar vesicle systems, and only limited data are available for the unilamellar microvesicles and other forms of lipid structures [for reviews, see Mabrey & Sturtevant (1979) , Bergelson (1978), and O'Neill & Richards (1980)].…”

mentioning

confidence: 99%

“…In this work, these enzyme-trigger-fused structures (to be called p-UMV) have been characterized together with other known forms of lipid vesicles, microvesicles, macrovesicles [by the method of Enoch & Strittmatter (1979)], and multilamellar vesicles. Although synthetic lecithins are widely used, DMPC vesicles are still poorly characterized regarding 31P NMR and DSC analyses [for reviews, see Mabrey & Sturtevant (1979), Bergelson (1978), and O'Neill & Richards (1980) ]. It is demonstrated that the p-UMV are unilamellar macrovesicles which can be useful for the functional study of this enzyme.…”

mentioning

confidence: 99%

Comparative study of an adenosine triphosphatase trigger-fused lipid vesicle and other vesicle forms of dimyristoylphosphatidylcholine

Dufour¹,

Nunnally²,

Buhle³

et al. 1981

Biochemistry

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Several known forms of bilayer vesicles of dimyristoylphosphatidylcholine exhibit the gel to liquid-crystalline phase transition in the temperature range convenient for membrane enzyme reconstitution studies. This warrants a systematic investigation of their physical characteristics and their phase transition behaviors. We have employed electron microscopy, gel chromatography, 31P nuclear magnetic resonance, differential scanning microcalorimetry, and fluorescence spectroscopy to determine several physical parameters of the limiting size microvesicle (260 +/- 40 A), the larger vesicle form (900 +/- 100A) of Enoch and Strittmatter [Enoch, H. G., & Strittmatter, P. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 145], the multilamellar vesicle, and, in particular, an ATPase-trigger-fused macrovesicle (950 +/- 200 A). This latter vesicle form was produced by a spontaneous fusion of the complex of the plasma membrane ATPase of Schizosaccharomyces pombe and the lipid microvesicles at a low ratio of enzyme to vesicle concentrations, and at a low temperature (around 10 degrees C). The ATPase-trigger-fused vesicles are unilamellar and have an intact ionic permeation barrier at 30 degrees C and a gel to liquid-crystalline transition temperature at 24.4 degrees C with a transition heat of 5.64 kcal/mol. Thus, this vesicle form should be a valuable tool for studying possible proton-pumping activity of this ATPase. In contrast to data found in the literature, which show lack of the pretransition for unilamellar microvesicles, we have observed the pretransition around 15 degrees C for all the vesicle forms examined. Moreover, the transition widths of unilamellar vesicles are much broader than those of the multilamellar vesicles, suggesting that in the latter system interlayer interactions may contribute to the cooperativity of the transition.

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“…1b) (Storey et al 1984;Pullin et al 1990;Coulson et al 1992). Resonances were assigned with reference to previous studies: peak 1: sugar phosphates, inorganic phosphate, phosphomonoesters or diesters including membrane lipids; peak 2: phosphoarginine, γATP and βADP; Peak 3: αATP and αADP; peak 4: βATP (O'Neill & Richards 1980;Gard et al 1985;Giblin et al 1986;Storey et al 1984;Cerdan & Seelig 1990;Pullin et al 1990;Wegener et al 1991). Peak 4 (βATP) provides the most unambiguous quantitation of ATP because it does not overlap with other resonances.…”

Section: Validation Of Nuclear Magnetic Resonance Proceduresmentioning

confidence: 99%

“…A failure of oxidative phosphorylation to meet the energetic needs of the cells can, thus, be detected by a decrease in the phosphagen pool and increase in inorganic phosphate. Levels of ATP, phosphagens and inorganic phosphate can be estimated using 31 P nuclear magnetic resonance (NMR) spectroscopy and used to reconstruct the energetic status of cells or whole organisms (O'Neill & Richards 1980). This sensitive and non-invasive technique has previously been applied in live insects and amphibians to illustrate a decline in ATP and corresponding loss of energetic homeostasis during severe cold exposure and freezing (Pullin et al 1990;Coulson et al 1992 ; Layne & Kennedy 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 The main goal of this study was to assess whether the ability to maintain energy status during cold exposure and recovery was altered by cold-hardiness adaptation in lines of D. melanogaster artificially selected for fast and s...…”

Section: Introductionmentioning

confidence: 99%

Cold adaptation does not alter ATP homeostasis during cold exposure in Drosophila melanogaster

Williams

Rocca

Edison

et al. 2018

Integrative Zoology

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In insects and other ectotherms, cold temperatures cause a coma resulting from loss of neuromuscular function, during which ionic and metabolic homeostasis are progressively lost. Cold adaptation improves homeostasis during cold exposure, but the ultimate targets of selection are still an open question. Cold acclimation and adaptation remodels mitochondrial metabolism in insects, suggesting that aerobic energy production during cold exposure could be a target of selection. Here, we test the hypothesis that cold adaptation improves the ability to maintain rates of aerobic energy production during cold exposure by using P NMR on live flies. Using lines of Drosophila melanogaster artificially selected for fast and slow recovery from a cold coma, we show that cold exposure does not lower ATP levels and that cold adaptation does not alter aerobic ATP production during cold exposure. Cold-hardy and cold-susceptible lines both experienced a brief transition to anaerobic metabolism during cooling, but this was rapidly reversed during cold exposure, suggesting that oxidative phosphorylation was sufficient to meet energy demands below the critical thermal minimum, even in cold-susceptible flies. We thus reject the hypothesis that performance under mild low temperatures is set by aerobic ATP supply limitations in D. melanogaster, excluding oxygen and capacity limitation as a weak link in energy supply. This work suggests that the modulations to mitochondrial metabolism resulting from cold acclimation or adaptation may arise from selection on a biosynthetic product(s) of those pathways rather than selection on ATP supply during cold exposure. This article is protected by copyright. All rights reserved.

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Biological 31P NMR Spectroscopy

Cited by 27 publications

References 308 publications

Chemical synthesis and characterization of 7-methylguanosine cap analogs

Chemical synthesis and characterization of 7-methylguanosine cap analogs

Comparative study of an adenosine triphosphatase trigger-fused lipid vesicle and other vesicle forms of dimyristoylphosphatidylcholine

Cold adaptation does not alter ATP homeostasis during cold exposure in Drosophila melanogaster

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