Biologic Treatments for Sports Injuries II Think Tank—Current Concepts, Future Research, and Barriers to Advancement, Part 1

LaPrade, Robert F.; Geeslin, Andrew G.; Murray, Iain R.; Musahl, Volker; Zlotnicki, Jason P.; Petrigliano, Frank A.; Mann, Barton J.

doi:10.1177/0363546516634674

Cited by 108 publications

(93 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Each phase is driven by different cell populations and several inflammatory proteins and growth factors. Some of these factors, including TGF‐ β, remain constant throughout the process, highlighting their key healing role .…”

Section: Introductionmentioning

confidence: 99%

Combination of biochemical and mechanical cues for tendon tissue engineering

Testa

Costantini

Fornetti

et al. 2017

J Cellular Molecular Medi

View full text Add to dashboard Cite

Tendinopathies negatively affect the life quality of millions of people in occupational and athletic settings, as well as the general population. Tendon healing is a slow process, often with insufficient results to restore complete endurance and functionality of the tissue. Tissue engineering, using tendon progenitors, artificial matrices and bioreactors for mechanical stimulation, could be an important approach for treating rips, fraying and tissue rupture. In our work, C3H10T1/2 murine fibroblast cell line was exposed to a combination of stimuli: a biochemical stimulus provided by Transforming Growth Factor Beta (TGF‐β) and Ascorbic Acid (AA); a three‐dimensional environment represented by PEGylated‐Fibrinogen (PEG‐Fibrinogen) biomimetic matrix; and a mechanical induction exploiting a custom bioreactor applying uniaxial stretching. In vitro analyses by immunofluorescence and mechanical testing revealed that the proposed combined approach favours the organization of a three‐dimensional tissue‐like structure promoting a remarkable arrangement of the cells and the neo‐extracellular matrix, reflecting into enhanced mechanical strength. The proposed method represents a novel approach for tendon tissue engineering, demonstrating how the combined effect of biochemical and mechanical stimuli ameliorates biological and mechanical properties of the artificial tissue compared to those obtained with single inducement.

show abstract

Section: Introductionmentioning

confidence: 99%

Combination of biochemical and mechanical cues for tendon tissue engineering

Testa

Costantini

Fornetti

et al. 2017

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…Because the use of bone marrow aspirate concentrate (BMAC) is currently approved by the United States Food and Drug Administration, it represents one of the few means for acquiring progenitor cells and growth factors for subsequent injection. [1][2][3][4] After density gradient centrifugation to remove red blood cells, granulocytes, immature myeloid precursors, and platelets, progenitor cells account for a small population within the bone marrow (0.001% to 0.01%). [5][6][7] However, a high concentration of growth factors, including platelet-derived growth factor, transforming growth factor-b, and bone morphogenetic proteins 2 and 7, which are reported to have anabolic and antiinflammatory effects, [8][9][10] are present in BMAC.…”

mentioning

confidence: 99%

Bone Marrow Aspirate Concentrate Harvesting and Processing Technique

Chahla

Mannava

Cinque

et al. 2017

Arthroscopy Techniques

Self Cite

116

View full text Add to dashboard Cite

Bone marrow obtained by iliac crest aspiration is a common source for harvesting mesenchymal stem cells, other progenitor cells, and associated cytokine/growth factors. Recent studies have reported good to excellent outcomes with the use of bone marrow aspirate concentrate (BMAC) for pain relief in the treatment of focal chondral lesions and osteoarthritis of the knee. However, the harvesting and processing technique are crucial to achieve satisfactory results. Several studies have examined outcomes after BMAC injection, with encouraging results, but there is a lack of consensus in terms of the frequency of injection, the amount of BMAC that is injected, and the timing of BMAC injections. The purpose of this Technical Note was to describe a standardized bone marrow aspiration harvesting technique and processing method. Bone marrow obtained by iliac crest aspiration is a common source for harvesting mesenchymal stem cells, other progenitor cells, and associated cytokine/ growth factors. Because the use of bone marrow aspirate concentrate (BMAC) is currently approved by the United States Food and Drug Administration, it represents one of the few means for acquiring progenitor cells and growth factors for subsequent injection.

show abstract

“…10-13 An increasing number of clinical trials evaluating the use of platelet rich plasma (PRP) across a wide range of applications have not supported promising initial in vitro and early clinical data. 14-16 While a number of clinicians have become disillusioned with PRP, it is possible that further analysis of emerging and published literature may reveal particular indications in which such therapies are effective. These new therapies are likely to follow the ‘technology/hype’ curve, whereas others have followed a more damped pattern such as metal-on-metal joint arthroplasties, 17,18 which has been cited as an example of Scott’s parabola.…”

Section: The Technology Curvementioning

confidence: 99%