Biologic Skin Substitutes and Their Applications in Dermatology

Cronin, Hyland; Goldstein, Glenn D.

doi:10.1111/j.1524-4725.2012.02561.x

Cited by 33 publications

(30 citation statements)

References 9 publications

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“…The establishment of a skin substitute that resembles healthy human skin for long-term use has not been accomplished yet although various skin substitutes have been tested with regard to their epithelial regeneration, likeliness for secondary bacterial infection and aesthetical outcome supporting the use of cultured skin substitutes in foot ulcers [8]. Furthermore the application of (autologous) keratinocytes to matrices has been complicated in handling, low cell viability, demanding in staff requirements and therefore expensive and less implemented in a clinical setting [9]. …”

Section: Introductionmentioning

confidence: 99%

Cultivation of Keratinocytes and Fibroblasts in a Three-Dimensional Bovine Collagen-Elastin Matrix (Matriderm®) and Application for Full Thickness Wound Coverage in Vivo

Killat¹,

Reimers²,

Choi³

et al. 2013

IJMS

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New skin substitutes for burn medicine or reconstructive surgery pose an important issue in plastic surgery. Matriderm® is a clinically approved three-dimensional bovine collagen-elastin matrix which is already used as a dermal substitute of full thickness burn wounds. The drawback of an avital matrix is the limited integration in full thickness skin defects, depending on the defect size. To further optimize this process, Matriderm® has also been studied as a matrix for tissue engineering of skin albeit long-term cultivation of the matrix with cells has been difficult. Cells have generally been seeded onto the matrix with high cell loss and minimal time-consuming migration. Here we developed a cell seeded skin equivalent after microtransfer of cells directly into the matrix. First, cells were cultured, and microinjected into Matriderm®. Then, cell viability in the matrix was determined by histology in vitro. As a next step, the skin substitute was applied in vivo into a full thickness rodent wound model. The wound coverage and healing was observed over a period of two weeks followed by histological examination assessing cell viability, proliferation and integration into the host. Viable and proliferating cells could be found throughout the entire matrix. The presented skin substitute resembles healthy skin in morphology and integrity. Based on this study, future investigations are planned to examine behaviour of epidermal stem cells injected into a collagen-elastin matrix under the aspects of establishment of stem cell niches and differentiation.

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Section: Introductionmentioning

confidence: 99%

Cultivation of Keratinocytes and Fibroblasts in a Three-Dimensional Bovine Collagen-Elastin Matrix (Matriderm®) and Application for Full Thickness Wound Coverage in Vivo

Killat¹,

Reimers²,

Choi³

et al. 2013

IJMS

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“…Additionally, a variety of skin substitutes are currently in use although reviews have favored porcine xenografts as a practical biologic dressing to support SIH. [8][9][10] Advantages and Disadvantages…”

Section: Executionmentioning

confidence: 99%

Secondary Intention Healing and Purse-String Closures

Lam

Lowe

Johnson

et al. 2015

Dermatologic Surgery

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The art of dermatologic surgery often requires a tailored approach to the patient and can involve a spectrum of closures, from the simplest to most complex. This variety not only provides more reconstruction options, but are also necessary to keep dermatologic surgery cost-effective. Certain locations or situations are more amenable for these two types of closures than others. The use of secondary intention healing, either alone or in conjunction with purse-string closures, should be in every dermatologic surgeon's armamentarium.

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“…Skin layer to be replaced: Subdivided into epidermal, dermal, dermal-epidermal composites B. Durability: Temporary and permanent C. Origin of grafting material: Biologic -those generated from biologic materials such as animal or human tissue (allogenic, autogenic, or xenogenic); synthetic -produced in the laboratory; biosynthetic -combination of synthetic and biologic elements. [4,5] Several skin substitutes are currently available for a variety of applications. Naturally occurring or biological materials like amnion, cadaveric skin allograft and porcine skin xenografts are used worldwide as temporary skin substitutes.…”

Section: Introduction Introductionmentioning

confidence: 99%

Skin substitutes in dermatology

Anish¹

2015

Indian J Dermatol Venereol Leprol

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Biologic Skin Substitutes and Their Applications in Dermatology

Cited by 33 publications

References 9 publications

Cultivation of Keratinocytes and Fibroblasts in a Three-Dimensional Bovine Collagen-Elastin Matrix (Matriderm®) and Application for Full Thickness Wound Coverage in Vivo

Cultivation of Keratinocytes and Fibroblasts in a Three-Dimensional Bovine Collagen-Elastin Matrix (Matriderm®) and Application for Full Thickness Wound Coverage in Vivo

Secondary Intention Healing and Purse-String Closures

Skin substitutes in dermatology

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