Biologic Modification of Animal Models of Intervertebral Disc Degeneration

Larson, James W.; Levicoff, Eric A.; Gilbertson, Lars G.; Kang, James D.

doi:10.2106/00004623-200604002-00017

Cited by 24 publications

(10 citation statements)

References 9 publications

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“…Disc degeneration often results from traumatic injury or occurs naturally with aging. This pathological condition is commonly attributed to increased degradation of aggrecan molecules, giving rise to significant alterations in disc biochemical composition and a loss of hydration [5]. The NP is thus rendered more fibrous in structure and content, which reduces nutrient diffusion and waste removal [6].…”

Section: Introductionmentioning

confidence: 99%

Characterization of novel photocrosslinked carboxymethylcellulose hydrogels for encapsulation of nucleus pulposus cells

2010

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Section: Introductionmentioning

confidence: 99%

Characterization of novel photocrosslinked carboxymethylcellulose hydrogels for encapsulation of nucleus pulposus cells

2010

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“…Obesity and tobacco are potential risk factors for degenerative IVD disease [10]. A genetic component has been implicated in degenerative IVD disease, but investigations into the genetic factors involved in the aetiology of this condition are still in their infancy [13,19,34].…”

Section: Introductionmentioning

confidence: 99%

Symptomatic disc herniation and serum lipid levels

et al. 2011

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Insufficient blood supply to the intervertebral disc (IVD) has been proposed to play a role as causative factor in IVD degeneration. There is an association between IVD diseases and increased risk of dying of ischaemic heart disease. Obesity and tobacco are potential risk factors for degenerative IVD disease. High blood cholesterol and triglycerides serum levels are risk factors for atherosclerosis, and could be responsible for a decreased in the blood supply to the already poor vascularized IVD. We performed a frequency-matched casecontrol study to determine the serum levels of patients with symptomatic herniated lumbar disc. We examined the fasting serum lipid levels in 384 subjects who were operated at our institution. Group 1 included 169 consecutive patients (115 men and 54 women; mean age: 59.1 years, range 29-85) who underwent surgery for symptomatic disc herniation. Group 2 (control group) included 169 patients (115 men and 54 women; mean age: 61 years, range 26-86) who underwent arthroscopic meniscectomy for a meniscal tear in the same period. These patients were frequency-matched by age (within 3 years) and gender with patients of Group 1. Sera were extracted from blood samples and the concentrations of total cholesterol (TC) and triglycerides (TG) were determined. When comparing the two groups, patients with symptomatic herniated lumbar disc showed statistically significant higher triglyceride concentration (P = 0.02) and total cholesterol concentration (P = 0.01). Serum lipid levels may be a risk factor for IVD pathology. An enhanced understanding of these factors holds the promise of new approaches to the prevention and management of IVD pathology.

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“…This pathological condition is commonly attributed to increased degradation of aggrecan molecules, giving rise to significant alterations in disc biochemical composition and a subsequent loss of hydration (Larson et al, 2006). The NP is thus rendered more fibrous in structure and content, which reduces nutrient diffusion and waste removal (Raj, 2008).…”

Section: Introductionmentioning

confidence: 99%

Serum‐free, chemically defined medium with TGF‐β₃ enhances functional properties of nucleus pulposus cell‐laden carboxymethylcellulose hydrogel constructs

Reza

Nicoll

2009

Biotech & Bioengineering

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Degeneration of the nucleus pulposus (NP) has been implicated as a major cause of low back pain. Tissue engineering strategies may provide a viable NP replacement therapy; however, culture conditions must be optimized to promote functional tissue development. In this study, a standard serum-containing medium formulation was compared to a chemically defined, serum-free medium to determine the effect on matrix elaboration and functional properties of NP cell-laden carboxymethylcellulose (CMC) hydrogels. Additionally, both media were further supplemented with transforming growth factor-beta 3 (TGF-beta(3)). Glycosaminoglycan (GAG) content increased in both TGF-beta(3)-treated groups and was highest for treated, serum-free constructs (9.46 +/- 1.51 microg GAG/mg wet weight), while there were no quantifiable GAGs in untreated serum-containing samples. Histology revealed uniform, interterritorial staining for chondroitin sulfate proteoglycan throughout the treated, serum-free constructs. Type II collagen content was greater in both serum-free groups and highest in treated, serum-free constructs. The equilibrium Young's modulus was highest in serum-free samples supplemented with TGF-beta(3) (18.54 +/- 1.92 kPa), and the equilibrium weight swelling ratio of these constructs approached that of the native NP tissue (22.19 +/- 0.46 vs. 19.94 +/- 3.09, respectively). Taken together, these results demonstrate enhanced functional matrix development by NP cells when cultured in CMC hydrogels maintained in serum-free, TGF-beta(3) supplemented medium, indicating the importance of medium formulation in NP construct development.

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Biologic Modification of Animal Models of Intervertebral Disc Degeneration

Cited by 24 publications

References 9 publications

Characterization of novel photocrosslinked carboxymethylcellulose hydrogels for encapsulation of nucleus pulposus cells

Characterization of novel photocrosslinked carboxymethylcellulose hydrogels for encapsulation of nucleus pulposus cells

Symptomatic disc herniation and serum lipid levels

Serum‐free, chemically defined medium with TGF‐β₃ enhances functional properties of nucleus pulposus cell‐laden carboxymethylcellulose hydrogel constructs

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Biologic Modification of Animal Models of Intervertebral Disc Degeneration

Cited by 24 publications

References 9 publications

Characterization of novel photocrosslinked carboxymethylcellulose hydrogels for encapsulation of nucleus pulposus cells

Characterization of novel photocrosslinked carboxymethylcellulose hydrogels for encapsulation of nucleus pulposus cells

Symptomatic disc herniation and serum lipid levels

Serum‐free, chemically defined medium with TGF‐β3 enhances functional properties of nucleus pulposus cell‐laden carboxymethylcellulose hydrogel constructs

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Product

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Serum‐free, chemically defined medium with TGF‐β₃ enhances functional properties of nucleus pulposus cell‐laden carboxymethylcellulose hydrogel constructs