Biologic and Synthetic Cellular and/or Tissue-Based Products and Smart Wound Dressings/Coverings

Abdo, James F.; Ortman, Holly

doi:10.1016/j.suc.2020.05.006

Cited by 10 publications

(6 citation statements)

References 37 publications

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“…Human skin is a multi-layered tissue in which a complex system of cells, glands, nerves and blood vessels cooperate to fulfill several important functions in our body [19,20].…”

Section: Introductionmentioning

confidence: 99%

Bioprinting Technology in Skin, Heart, Pancreas and Cartilage Tissues: Progress and Challenges in Clinical Practice

Piazza

Pandolfi

Cacciotti

et al. 2021

IJERPH

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Bioprinting is an emerging additive manufacturing technique which shows an outstanding potential for shaping customized functional substitutes for tissue engineering. Its introduction into the clinical space in order to replace injured organs could ideally overcome the limitations faced with allografts. Presently, even though there have been years of prolific research in the field, there is a wide gap to bridge in order to bring bioprinting from “bench to bedside”. This is due to the fact that bioprinted designs have not yet reached the complexity required for clinical use, nor have clear GMP (good manufacturing practices) rules or precise regulatory guidelines been established. This review provides an overview of some of the most recent and remarkable achievements for skin, heart, pancreas and cartilage bioprinting breakthroughs while highlighting the critical shortcomings for each tissue type which is keeping this technique from becoming widespread reality.

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“…Human skin is a multi-layered tissue in which a complex system of cells, glands, nerves and blood vessels cooperate to fulfill several important functions in our body [19,20].…”

Section: Introductionmentioning

confidence: 99%

Bioprinting Technology in Skin, Heart, Pancreas and Cartilage Tissues: Progress and Challenges in Clinical Practice

Piazza

Pandolfi

Cacciotti

et al. 2021

IJERPH

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“…Biologic wound matrices are a heterogeneous group of tissue substitutes with biological and sometimes synthetic elements that can be used to cover wounds, while mitigating the complications associated with autologous flaps. [9][10][11][12][13] Currently, there are more than 70 commercially available skin substitutes as of 2020 for the treatment of acute and chronic wounds with different indications, and the research on this field is continuously expanding. [14][15][16][17] Despite this extensive research on novel products, there is a lack of a controlled and affordable testbed in the preclinical setting to reflect the challenging clinical scenario of an exposed avascular structure in the wound bed.…”

mentioning

confidence: 99%

“…To mitigate current shortcomings of free tissue transfer, tissue engineered alternatives are being explored. Biologic wound matrices are a heterogeneous group of tissue substitutes with biological and sometimes synthetic elements that can be used to cover wounds, while mitigating the complications associated with autologous flaps 9–13 . Currently, there are more than 70 commercially available skin substitutes as of 2020 for the treatment of acute and chronic wounds with different indications, and the research on this field is continuously expanding 14–17 .…”

mentioning

confidence: 99%

A Model to Study Wound Healing Over Exposed Avascular Structures in Rodents With a 3D-Printed Wound Frame

Bengur,

Komatsu,

Loder

et al. 2024

Ann Plast Surg

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Background Soft tissue defects with exposed avascular structures require reconstruction with well-vascularized tissues. Extensive research is ongoing to explore tissue engineered products that provide durable coverage. However, there is a lack of controlled and affordable testbeds in the preclinical setting to reflect this challenging clinical scenario. We aimed to address this gap in the literature and develop a feasible and easily reproducible model in rodents that reflects an avascular structure in the wound bed. Methods We created 20 × 20 mm full thickness wounds on the dorsal skin of Lewis rats and secured 0.5-mm-thick silicone sheets of varying sizes to the wound bed. A 3D-printed wound frame was designed to isolate the wound environment. Skin graft and free flap survival along with exposure of the underlying silicone was assessed. Rats were followed for 4 weeks with weekly dressing changes and photography. Samples were retrieved at the endpoint for tissue viability and histologic analysis. Results The total wound surface area was constant throughout the duration of the experiment in all groups and the wound frames were well tolerated. The portion of the skin graft without underlying silicone demonstrated integration with the underlying fascia and a histologically intact epidermis. Gradual necrosis of the portion of the skin graft overlying the silicone sheet was observed with varying sizes of the silicone sheet. When the size of the silicone sheet was reduced from 50% of the wound surface area, the portion surviving over the silicone sheet increased at the 4-week timepoint. The free flap provided complete coverage over the silicone sheet. Conclusion We developed a novel model of rodent wound healing to maintain the same wound size and isolate the wound environment for up to 4 weeks. This model is clinically relevant to a complex wound with an avascular structure in the wound bed. Skin grafts failed to completely cover increasing sizes of the avascular structure, whereas the free flap was able to provide viable coverage. This cost-effective model will establish an easily reproducible platform to evaluate more complex bioengineered wound coverage solutions.

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“…Использовали два режима воздействия температурой на формирующийся регенерат: +8 °С («холод»), +42 °С («тепло»), в «Контроле» -+33 °С. Материал забирали на 3,7,10,14,20,30-е сут опыта, фиксировали в 10 % нейтральном формалине, заливали в парафин. Срезы окрашивали гематоксилином Майера и эозином.…”

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“…Известные способы ускорения заживления ран, ожогов, травм направлены на стимуляцию биологических потенций клеток, участвующих в репарации. Состояние раневого дефекта на различных стадиях восстановления нарушенной структуры зависит от активности регуляторных факторов локального и дистантного характера действия, способа обработки и клинического ведения раневого дефекта [1][2][3][4][5].…”

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Morphogenesis of Skin Regenerate and Local Factors of Its Regulation

Shidin,

Aptekar',

Akhmatov

et al. 2023

Ulyanovsk Medico-biological Journal

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Regeneration of the skin and its derivatives after injuries occurs in one of two ways: cutaneous (restitution) or dermal (substitution). However, there are three healing mechanisms: primary intention, secondary intention and healing by a scab. Regeneration activity correlates with environmental factors, stimulants, compliance with the chronovector of involvement in the cell regenerate of all differon derivatives, involved in histo- and organogenesis in the affected area. The aim of the study is to identify the importance of promoter cells, temperature and Eikovit gel at different stages of thermal burn wound healing, contact dermatitis and wound skin defects and to determine the stage of provisional regenerate transformation into the definitive state. Materials and Methods. The formation of reparative skin regenerate was studied on outbred male mice weighing 25±5 g (n=126). Different injuries to the skin of the back were modelled. All mice were divided into 4 groups: Full-thickness wound, Thermal burn, Contact dermatitis, and Control. Thermal damage was carried out with Tertsik RS232C device (Russia), module area – 1 cm2, exposure time – 3 minutes, and temperature – 80 °C. Contact dermatitis was modeled by rubbing a 0.5 % alcohol-acetone solution of 2,4-dinitrochlorobenzene (2,4-DNCB) into the skin. The skin wound was cut out using an oval stencil (3×4 mm). We used three temperature modes to influence the developing regenerate: +8 °C (cold), +42 °C (heat), and +33 °C (control). Sampling was conducted on days 3, 7, 10, 14, 20, 30 of the experiment, fixed in 10 % neutral formalin, and embedded in paraffin. Sections were stained with Mayer's hematoxylin and eosin. Immunohistochemistry detected CD1-alpha, CD3, and CD31. Proliferative activity was monitored by Ki-67-positive cells. Results. The convergence chronovector of immunocompetent cells during skin wound healing ensures the restitution of skin components and its derivatives. One of the signs of the correct chronovector direction is the formation of a provisional level substrate and its further transformation into a definitive state. Convergence desynchronosis can lead to dermal healing and substitution.

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Biologic and Synthetic Cellular and/or Tissue-Based Products and Smart Wound Dressings/Coverings

Cited by 10 publications

References 37 publications

Bioprinting Technology in Skin, Heart, Pancreas and Cartilage Tissues: Progress and Challenges in Clinical Practice

Bioprinting Technology in Skin, Heart, Pancreas and Cartilage Tissues: Progress and Challenges in Clinical Practice

A Model to Study Wound Healing Over Exposed Avascular Structures in Rodents With a 3D-Printed Wound Frame

Morphogenesis of Skin Regenerate and Local Factors of Its Regulation

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