Biolistic Transformation of Wheat

Tian, Bin; Navia-Urrutia, Monica; Chen, Yueying; Brungardt, Jordan; Trick, Harold N.

doi:10.1007/978-1-4939-8778-8_9

Cited by 12 publications

(5 citation statements)

References 15 publications

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“…The FgTRI101 expression construct and the pAHC20 ( Christensen and Quail, 1996 ) containing the bar gene were co-bombarded into selected wheat embryogenic calli with a 1:1 ratio. Biolistic transformation was conducted with a particle inflow gun and then tissue culture and plant regeneration were performed ( Tian et al., 2019 ). Leaves of recovered plants grown in soil were screened for herbicide resistance by brushing a 0.2% v/v Liberty (glufosinate) solution (AgroEvo USA, Wilmington, DE).…”

Section: Methodsmentioning

confidence: 99%

Reduction of Fusarium head blight and trichothecene contamination in transgenic wheat expressing Fusarium graminearum trichothecene 3-O-acetyltransferase

Yulfo-Soto,

McCormick,

Chen

et al. 2024

Front. Plant Sci.

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Fusarium graminearum, the causal agent of Fusarium head blight (FHB), produces various mycotoxins that contaminate wheat grains and cause profound health problems in humans and animals. Deoxynivalenol (DON) is the most common trichothecene found in contaminated grains. Our previous study showed that Arabidopsis-expressing F. graminearum trichothecene 3-O-acetyltransferase (FgTRI101) converted DON to 3-acetyldeoxynivalenol (3-ADON) and excreted it outside of Arabidopsis cells. To determine if wheat can convert and excrete 3-ADON and reduce FHB and DON contamination, FgTRI101 was cloned and introduced into wheat cv Bobwhite. Four independent transgenic lines containing FgTRI101 were identified. Gene expression studies showed that FgTRI101 was highly expressed in wheat leaf and spike tissues in the transgenic line FgTri101-1606. The seedlings of two FgTri101 transgenic wheat lines (FgTri101-1606 and 1651) grew significantly longer roots than the controls on media containing 5 µg/mL DON; however, the 3-ADON conversion and excretion was detected inconsistently in the seedlings of FgTri101-1606. Further analyses did not detect 3-ADON or other possible DON-related products in FgTri101-1606 seedlings after adding deuterium-labeled DON into the growth media. FgTri101-transgenic wheat plants showed significantly enhanced FHB resistance and lower DON content after they were infected with F. graminearum, but 3-ADON was not detected. Our study suggests that it is promising to utilize FgTRI101, a gene that the fungus uses for self-protection, for managing FHB and mycotoxin in wheat production.

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Section: Methodsmentioning

confidence: 99%

Reduction of Fusarium head blight and trichothecene contamination in transgenic wheat expressing Fusarium graminearum trichothecene 3-O-acetyltransferase

Yulfo-Soto,

McCormick,

Chen

et al. 2024

Front. Plant Sci.

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“…The expression constructs and the pAHC20 vector 55 containing the bar gene were co-bombarded with 1:1 ratio into selected embryogenic calli. A biolistic approach using a particle inflow gun and following tissue culture protocols were performed for transformation 61 , 62 . Recovered plants in soil were screened for herbicide resistance by brushing a 0.2% v/v Liberty (glufosinate) solution (Bayer CropScience, USA) on leaves.…”

Section: Methodsmentioning

confidence: 99%

Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii

et al. 2022

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The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMMYT international wheat breeding program with resulting cultivars deployed in several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for Lr42. Overexpression of a nucleotide-binding site leucine-rich repeat (NLR) gene AET1Gv20040300 induces strong resistance to leaf rust in wheat and a mutation of the gene disrupted the resistance. The Lr42 resistance allele is rare in Ae. tauschii and likely arose from ectopic recombination. Cloning of Lr42 provides diagnostic markers and over 1000 CIMMYT wheat lines carrying Lr42 have been developed documenting its widespread use and impact in crop improvement.

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“…[129][130][131][132][133][134] Alternatively, the recombinant DNA/RNA can be directly delivered by the biolistic method, employing gold particles or tungsten covered with a linear expression cassette or an expression vector harbouring the chimeric gene. 135 The biolistic method causes multiple transgene integration, although lower concentrations (in nanograms) lead to an increased frequency of plants with single-copy. [136][137][138] The direct transfer of chimeric gene into protoplasts (plant cells with an enzymatically removed cell wall) mediated by polyethylene glycol (PEG), represents another option applied for several plant species.…”

Section: Preparation Of Transgenic Plantsmentioning

confidence: 99%

“…This can be exploited to deliver any chimeric genes into the plant cells of choice using existing protocols aiming at the highest transformation efficiency 129–134 . Alternatively, the recombinant DNA/RNA can be directly delivered by the biolistic method, employing gold particles or tungsten covered with a linear expression cassette or an expression vector harbouring the chimeric gene 135 . The biolistic method causes multiple transgene integration, although lower concentrations (in nanograms) lead to an increased frequency of plants with single‐copy 136–138 .…”

Section: Molecular Farming Of Fish Vaccinesmentioning

confidence: 99%

Molecular farming: Expanding the field of edible vaccines for sustainable fish aquaculture

Mičúchová

Piačková

Frébort

et al. 2022

Reviews in Aquaculture

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Vaccines represent one of the most promising strategies for mitigating economic losses imposed by infectious diseases to global aquaculture. While the majority of commercial vaccines employ injection as the main route of delivery, a substantial body of research explores other avenues for the stimulation of protective immunity. Oral vaccines, representing the most favourable and convenient solution for the aquaculture industry, have been at the forefront of scientific interest for the last couple of decades. The orchestrated effort resulted in identifying the main roadblocks on the path to an ideal edible vaccine and provided several ingenious solutions improving the activation of immunity, ensuring the stability of administered antigens and their uptake upon the passage through the stomach. In the presented review, we describe advances in the available knowledge of the processes prerequisite for developing protective mucosal vaccines and focus readers' attention on the untapped potential of plant‐based production systems in this effort. We propose that these approaches not only meet production demands but also fulfil all requirements of an oral vaccine, addressing all the obstacles, previously solved via separate strategies, in one platform. Thus, combined with the available knowledge of molecular adjuvants and produced for a fraction of the cost, plant‐based production, so‐called molecular farming, is an ideal candidate for vaccine development, paving the road to sustainable aquaculture.

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Biolistic Transformation of Wheat

Cited by 12 publications

References 15 publications

Reduction of Fusarium head blight and trichothecene contamination in transgenic wheat expressing Fusarium graminearum trichothecene 3-O-acetyltransferase

Reduction of Fusarium head blight and trichothecene contamination in transgenic wheat expressing Fusarium graminearum trichothecene 3-O-acetyltransferase

Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii

Molecular farming: Expanding the field of edible vaccines for sustainable fish aquaculture

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