Abstract:Among different explants, cotyledon showed highest regeneration and number of buds per explant on all media combinations. However, highest regeneration potential was observed on MS fortified with 3.0 mgl -1 BAP (80.36%), but number of buds per explant were highest (21.76) on MS media supplemented with 2.5 mgl -1 BAP+ 1.0 mgl -1 Kin. Plant regeneration of cotyledons was completely checked with a selection pressure of 15ppm to 25ppm hygromycin for untranformed explants. But the selection efficacy of transformed … Show more
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