Biolistic Mediated Sorghum (Sorghum bicolor L. Moench) Transformation via Mannose and Bialaphos Based Selection Systems

Grootboom, AW; Mkhonza, NL; O’Kennedy, Martha M.; Chakauya, Ereck; Kunert, K.; Chikwamba, Rachel

doi:10.3923/ijb.2010.89.94

Cited by 32 publications

(26 citation statements)

References 23 publications

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“…In recent years, plant biotechnology, including molecular genetics and genomics as well as plant transformation, has provided a powerful means to supplement traditional breeding approaches. Plant transformation has a unique role in varietal improvement and offers a much faster approach to accomplish genetic gains for various traits (Gurel et al 2009;Grootboom et al 2010). These gains will contribute to both food and biofuel industries as they relate to sorghum production.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, efforts have been made to improve sorghum varieties to reduce the impacts of these limiting factors on sorghum agronomical performance. To date, most sorghum varietal improvements have been achieved through conventional breeding (Grootboom et al 2010). However, traditional breeding for crop improvement has several limitations, including its inability to sustain yield and productivity indefinitely (Vasil 1994).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sorghum Transformation: Achievements, Challenges, and Perspectives

Azhakanandam

Zhang

2015

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sorghum Transformation: Achievements, Challenges, and Perspectives

Azhakanandam

Zhang

2015

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

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“…PCR screening showed that 79 of the 92 phosphinothricin resistant plants contained either one or both E1 or cbh1, 11 being E1 single transgenics, 12 cbh1 single transgenics and 56 co-transformed with both cellulase genes. Despite the advantage of stringent selection for resistant plants, the generation of 13 escapes in the experiment indicated that the bar selection system seems to be leaky in some cereal transformations (Grootboom et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Co-expression of microbial cellulases in transgenic wheat as a potential source for cellulosic ethanol production

Hussain

Nugent

Stevenson

et al. 2015

Plant Biotechnology

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Crop residues are produced in abundance in agriculture and forestry and are a potential source of lignocellulosic biomass for ethanol production. Recent research has included the heterologous expression in transgenic plants of genes encoding cellulases to produce fermentable sugars from plant biomass. In this study, transgenic wheat lines were generated co-expressing the genes for an endoglucanase 1 (E1) of Acidothermus cellulolyticus and cellobiohydrolase 1 (CBH1) of Trichoderma reesei. Both genes were under the control of a wheat Rubisco small subunit promoter (RbcS). Transgenic wheat leaves accumulated apoplast targeted E1 and CBH1 proteins at levels up to 1 and 0.5% respectively of total soluble protein as determined by immunoblotting. Transgenic plants co-expressing E1 and CBH1 were analysed by a 4-methylumbelliferyl-β-D-cellobioside (MUC) assay and enzymatic activity was detected up to 92 nmol 4-MU/mg tsp/min.

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“…To select the transformed calli, the herbicideresistant gene, bar (Thompson et al, 1987) was employed as a selectable marker. Selection of bialaphos-resistant callus is often conducted at 2 to 3 mg/L bialaphos (Casas et al, 1993;Frame et al, 2002;Grootboom et al, 2010). Bialaphos, as selection agent, has been found to hinder regeneration capacity of sorghum (Casas et al, 1993;Gao et al, 2005a;Lu et al, 2009;Grootboom et al, 2010).…”

Section: Sorghum Transformation and Regenerationmentioning

confidence: 99%

The impact of over-expression of NPK1 gene on growth and yield of sorghum under drought stress

Assem¹,

Zamzam²,

Saad³

et al. 2017

Afr. J. Biotechnol.

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Sorghum bicolor L. is an important crop in many tropical regions, yet it has received little attention in applying modern biotechnologies for improvement due to transformation difficulties. Drought is among the most important factors limiting sorghum productivity. The Nicotina Protein Kinase 1 (NPK1) gene confers tolerance to adverse environmental conditions. The present work was conducted to determine the amenability of sorghum genotypes to Agrobacterium-mediated transformation with the NPK1 gene and to characterize the role of that gene in S. bicolor. The NPK1 gene along with the bar gene was successfully introduced into two sorghum genotypes: Dorado and SPGM94021. The transformation frequencies were 1.49 and 1.79% for Dorado and SPGM94021, respectively. Stable integration of the transgenes in T0 and T1 plants was confirmed through polymerase chain reaction (PCR) and Southern blotting. The NPK1 gene expression was measured through real-time-PCR. T1 plants tested in vitro for tolerance to mannitol osmotic stress maintaining a higher growth rate and showed increased tolerance to stress conditions compared to the nontransgenic plants. The transgenic sorghum had a significantly higher kernel weight under drought stress conditions than the control plants. Accordingly, the NPK1 gene might induce a mechanism that protects sorghum plants against possible water-deficiency stress conditions.

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Biolistic Mediated Sorghum (Sorghum bicolor L. Moench) Transformation via Mannose and Bialaphos Based Selection Systems

Cited by 32 publications

References 23 publications

Sorghum Transformation: Achievements, Challenges, and Perspectives

Sorghum Transformation: Achievements, Challenges, and Perspectives

Co-expression of microbial cellulases in transgenic wheat as a potential source for cellulosic ethanol production

The impact of over-expression of NPK1 gene on growth and yield of sorghum under drought stress

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