Bioinspired synthesis of polydopamine/Ag nanocomposite particles with antibacterial activities

Wu, Chengjiao; Zhang, Guoxing; Xia, Tian; Li, Zhenni; Zhao, Kai; Deng, Ziwei; Guo, Dingzong; Peng, Bo

doi:10.1016/j.msec.2015.05.032

Cited by 93 publications

(69 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results indicate that the antibacterial activity of EM/AgNPs is strong enough to protect skin wounds from bacterial infection, which may efficiently enhance wound healing. Interestingly, consistent with previous studies, we also observed that EM/AgNPs showed better antibacterial activity against E. coli than S. aureus , which may due to the different cellular wall structures of gram-negative and gram-positive bacteria 7, 48 .…”

Section: Discussionsupporting

confidence: 92%

“…To meet this demand, an ideal wound dressing should exhibit good biocompatibility and appropriate porosity, as well as effective antibacterial activity, to accelerate wound healing 5, 6 . However, few clinical products can meet all these needs due to their mono-functionality, relatively complicated preparation procedures, and high cost; thus, developing such an ideal wound dressing for tissue regeneration remains a challenge 5, 7, 8 .…”

Section: Introductionmentioning

confidence: 99%

“…The mussel-inspired DA has super adhesive activity and can create a polymerized layer on various surfaces of material 31, 35 . In addition, PD exhibits good biocompatibility and low toxicity, which are beneficial for cell and tissue regeneration 7, 36 . Therefore, it is feasible to use DA to synthesize controlled concentrations of AgNPs on the surface of EM, although the cytotoxicity of treatment with different concentrations and under different conditions has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nano-silver-decorated microfibrous eggshell membrane: processing, cytotoxicity assessment and optimization, antibacterial activity and wound healing

Menglong

Luo

Wang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

An ideal wound dressing can both promote wound healing and prevent bacterial infection. Here, we report a potential dressing prepared by incorporating an optimized concentration of silver nanoparticles (AgNPs) into the microfibers of a natural eggshell membrane (EM) using environmentally friendly and mussel-inspired dopamine. Briefly, acid-treated EM was used as a porous membrane for polydopamine-reduced AgNPs synthesis. To obtain the optimal cytocompatible silver concentration, cellular attachment and MTT assay were performed with different concentrations of AgNPs. The morphology of the EM and AgNPs was confirmed by scanning electronic microscopy, scanning transmission electronic microscopy and Fourier transform infrared spectroscopy. The synthesized EM/AgNPs exhibited steady and safe AgNPs release, which was further tested for antibacterial activity against Escherichia coli and Staphylococcus aureus by disc diffusion method and bacterial suspension assay. Finally, in a murine full-thickness skin wound model, we found that EM/AgNPs could promote re-epithelialization, granulation tissue formation and wound healing via enhancing cell proliferation, as demonstrated by the expression of proliferating cell nuclear antigen (PCNA), and controlling inflammation response, as demonstrated by the expression of interleukin-1β (IL-1β). These findings suggest that EM/AgNPs may have a promising application in wound management.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nano-silver-decorated microfibrous eggshell membrane: processing, cytotoxicity assessment and optimization, antibacterial activity and wound healing

Menglong

Luo

Wang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…From the XPS profile (Figure S3, Supporting Information), it was determined the enlarged Ag 3d signal composed of two individual peaks at 368 and 374 eV with a spin‐orbit separation of 6.0 eV, which were in accordance with the binding energies of

{Ag}_{3 {normald}_{3 / 2}}

and

{Ag}_{3 {normald}_{5 / 2}}

, respectively . From the XRD pattern (Figure S4, Supporting Information), the four peaks at the sites of 38.12°, 44.2°, 64.45°, and 77.38° were assigned to the (1 1 1), (2 0 0), (2 2 0), and (3 1 1) crystal faces of elemental silver, respectively . After being soaked in 5SBF, Ca 2p (347 eV) and P 2s (191 eV) signals were detected on both PEG10%+D+Ag+B and PEG10%+D+B microspheres (Figure S3, Supporting Information).…”

Section: Resultsmentioning

confidence: 77%

Bioresorbable Microspheres with Surface‐Loaded Nanosilver and Apatite as Dual‐Functional Injectable Cell Carriers for Bone Regeneration

Wei

Yuan

Cai

et al. 2018

Macromol. Rapid Commun.

View full text Add to dashboard Cite

Bone defect repair, especially in terms of infection, remains a big challenge in clinical therapy. To meet clinical requirements, the dual-functional strategy including antibacterial activity and strong osteogenesis effect is a promising approach in bone tissue engineering. In the present study, bioresorbable porous-structured microspheres are fabricated from amphiphilic block copolymer composed of poly(l-lactide) and poly(ethyl glycol) blocks. After being surface coated with mussel-inspired polydopamine, the microspheres are loaded with nanosilver via reduction of silver nitrate and apatite via biomineralization in sequence. The resulting composite microspheres are systematically characterized by in vitro coculturing with rat bone mesenchymal stromal cells and Staphylococcus aureus to evaluate cytotoxicity and antibacterial activity. In vivo evaluation is carried out by filling the composite microspheres into infected cranial defects in a rat model. The primary results indicate that the dual-purpose microspheres have the capacity to defeat infection while promoting bone regeneration.

show abstract

“…Nevertheless, the conventional physical methods are low yield and high energy consumption, and the general chemical methods are expensive and toxic to environment. [14][15][16] This makes an easy and environmentally friendly way to generate NS, which is very desirable for the extended application of NS. Recently, dopamine (DA) is found to be gently self-polymerized under alkaline condition because of its mussel adhesive property, and the formed polydopamine (PD) coating possesses super adhesive ability and strong reduction property.…”

mentioning

confidence: 99%

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Liu¹,

Luo²,

Wang³

et al. 2017

IJN

View full text Add to dashboard Cite

Bacterial infection is a major hurdle to wound healing, and the overuse of antibiotics have led to global issue, such as emergence of multidrug-resistant bacteria, even “super bacteria”. On the contrary, nanosilver (NS) can kill bacteria without causing resistant bacterial strains. In this study, NS was simply generated in situ on the polycaprolactone (PCL) nanofibrous mesh using an environmentally benign and mussel-inspired dopamine (DA). Scanning electron microscopy showed that NS uniformly formed on the nanofibers of PCL mesh. Fourier transform infrared spectroscopy revealed the step-by-step preparation of pristine PCL mesh, including DA coating and NS formation, which were further verified by water contact angle changing from hydrophobic to hydrophilic. To optimize the NS dose, the antibacterial activity of PCL/NS against Staphylococcus aureus , Escherichia coli and Acinetobacter baumannii was detected by bacterial suspension assay, and the cytotoxicity of NS was evaluated using cellular morphology observation and Cell Counting Kit-8 (CCK8) assay. Then, inductively coupled plasma atomic emission spectrometry exhibited that the optimized PCL/NS had a safe and sustained silver release. Moreover, PCL/NS could effectively inhibit bacterial infection in an infectious murine full-thickness skin wound model. As demonstrated by the enhanced level of proliferating cell nuclear antigen (PCNA) in keratinocytes and longer length of neo-formed epidermis, PCL/NS accelerated wound healing by promoting re-epithelialization via enhancing keratinocyte proliferation in infectious wounds.

show abstract

Bioinspired synthesis of polydopamine/Ag nanocomposite particles with antibacterial activities

Cited by 93 publications

References 42 publications

Nano-silver-decorated microfibrous eggshell membrane: processing, cytotoxicity assessment and optimization, antibacterial activity and wound healing

Nano-silver-decorated microfibrous eggshell membrane: processing, cytotoxicity assessment and optimization, antibacterial activity and wound healing

Bioresorbable Microspheres with Surface‐Loaded Nanosilver and Apatite as Dual‐Functional Injectable Cell Carriers for Bone Regeneration

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Contact Info

Product

Resources

About