Bioinspired self-assembly of tyrosinase-modified silicatein and fluorescent core–shell silica spheres

Elkhooly, Tarek A.; Müller, Wernér E.G.; Wang, X; Tremel, Wolfgang; Isbert, Simone; Wiens, Matthias

doi:10.1088/1748-3182/9/4/044001

Cited by 7 publications

(2 citation statements)

References 42 publications

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“…Biosilica formed by silicatein has been validated as a promising matrix to immobilize biomolecules in many studies. , Immobilized silicatein could act as an interfacial catalyst to form silica on some material and ultimately facilitate the fabrication of hybrid materials . Recombinant silicateins that had been bioengineered with affinity tags to facilitate adhesion on various carrier surfaces have been studied to fabricate hybrid materials. − However, silicatein shows aggregation properties, which makes its handling difficult in various applications. Therefore, the solubility of silicatein in aqueous media should be improved until it is bound to the target material.…”

Section: Introductionmentioning

confidence: 99%

Biological Route to Fabricate Silica on Cellulose Using Immobilized Silicatein Fused with a Carbohydrate-Binding Module

et al. 2020

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Silicatein is an enzyme capable of catalyzing silica formation under mild conditions and is a promising catalyst for the fabrication of biohybrid materials. However, unfavorable aggregation of silicatein makes it unsuitable for use in material fabrication. In this study, a soluble protein tag (ProS2) and a carbohydrate-binding module (CBM) were used to develop a soluble and cellulose-binding fusion silicatein, ProS2-Sil-CBM, which can be efficiently immobilized on cellulose to form silica on it. ProS2-Sil-CBM was soluble in aqueous media and strongly bound to cellulose. ProS2-Sil-CBM bound on cellulose catalyzed the formation of a silica layer on the cellulose in the presence of tetraethyl orthosilicate as the substrate. Scanning electron microscopy (SEM) and surface elemental analysis confirmed the formation of silica on cellulose. This technique can be used to fabricate inorganic–organic hybrid materials to immobilize biomolecules and can be applied to develop novel biocatalytic systems, biosensors, and tissue culture scaffolds.

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Section: Introductionmentioning

confidence: 99%

Biological Route to Fabricate Silica on Cellulose Using Immobilized Silicatein Fused with a Carbohydrate-Binding Module

et al. 2020

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“…Recently, several studies have explored the potential of the mussel bio–inspired dopamine to undergo self–polymerization under mild conditions forming polydopamine (PDA) [27]. Indeed, polydopamine possesses highly adhesion capacity on not only inorganic surfaces such as silica and magnetic nanoparticles but also on organic surfaces such as polymeric matrices and fabrics [28, 29, 30, 31, 32]. The adhesion force of PDA is derived from its catechol groups that form hydrogen bonding with the coated surfaces.…”

Section: Introductionmentioning

confidence: 99%

Facile coating of urinary catheter with bio–inspired antibacterial coating

Yassin

Elkhooly

Elsherbiny

et al. 2019

Heliyon

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A B S T R A C TFormation of bacterial biofilm on indwelling urinary catheters usually causes catheter-associated urinary tract infections (CAUTIs) that represent high percent of nosocomial infections worldwide. Therefore, coating urinary catheter with antibacterial and antifouling coating using facile technique is in great demand. In this study, commercial urinary catheter was coated with a layer of the self-polymerized polydopamine which acts as active platform for the in situ formation of silver nanoparticle (AgNPs) on catheter surface. The formed coating was intensively characterized using spectroscopic and microscopic techniques. The coated catheter has the potential to release silver ion in a sustained manner with a concentration of about 2-4 μg ml À1 . Disk diffusion test and colony forming unites assay verified the significant bactericidal potential of the AgNPs coated catheter against both gram-positive and gram-negative bacteria as a consequence of silver ion release. In contrast to commercial catheter, the AgNPs coated catheter prevented the adherence of bacterial cells and biofilm formation on their surfaces. Interestingly, scanning electron microscope investigations showed that AgNPs coated catheter possess greater antifouling potential against gram-positive bacteria than against gram-negative bacteria. Overall, the remarkable antibacterial and antifouling potential of the coated catheter supported the use of such facile approach for coating of different medical devices for the prevention of nosocomial infections.

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Designed Multifunctional Spider Silk Enabled by Genetically Encoded Click Chemistry

Jiang

Tan

Fang

et al. 2023

Adv Funct Materials

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Spider silk is recognized for its exceptional mechanical properties and biocompatibility, making it a versatile platform for developing functional materials. In this study, a modular functionalization strategy for recombinant spider silk is presented using SpyTag/SpyCatcher chemistry, a prototype of genetically encoded click chemistry. The approach involves AlphaFold2‐aided design of SpyTagged spider silk coupled with bacterial expression and biomimetic spinning, enabling the decoration of silk with various SpyCatcher‐fusion motifs, such as fluorescent proteins, enzymes, and cell‐binding ligands. The silk threads can be coated with a silica layer using silicatein, an enzyme for silicification, resulting in a hybrid inorganic–organic 1D material. The threads installed with RGD or laminin cell‐binding ligands lead to enhanced endothelial cell attachment and proliferation. These findings demonstrate a straightforward yet powerful approach to 1D protein materials.

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Bioinspired self-assembly of tyrosinase-modified silicatein and fluorescent core–shell silica spheres

Cited by 7 publications

References 42 publications

Biological Route to Fabricate Silica on Cellulose Using Immobilized Silicatein Fused with a Carbohydrate-Binding Module

Biological Route to Fabricate Silica on Cellulose Using Immobilized Silicatein Fused with a Carbohydrate-Binding Module

Facile coating of urinary catheter with bio–inspired antibacterial coating

Designed Multifunctional Spider Silk Enabled by Genetically Encoded Click Chemistry

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