Bioinspired Nanocorals with Decoupled Cellular Targeting and Sensing Functionality

Wu, Liz Y.; Ross, Benjamin M.; Hong, SoonGweon; Lee, Luke P.

doi:10.1002/smll.200901604

Cited by 131 publications

(125 citation statements)

References 37 publications

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“…130 An interesting and novel type of stand-alone cellular SERS tag called a nanocoral was reported. 185 As shown in Figure 8, half of the nanocoral's surface was coated with highly roughened gold to increase its reporter adsorption capacity and create a high density of SERS hot spots, and the blank polystyrene hemisphere could be functionalized with targeting moieties to specific cells. Therefore, the targeting and sensing mechanisms for a particular experiment could be separately engineered.…”

Section: Pathogen Detectionmentioning

confidence: 99%

SERS Tags: Novel Optical Nanoprobes for Bioanalysis

2012

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Section: Pathogen Detectionmentioning

confidence: 99%

SERS Tags: Novel Optical Nanoprobes for Bioanalysis

2012

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“…These synthesis methods utilize kinetic control over nucleation of the nano-cluster through a carefully determined polymer concentration for steric stabilization, which limits their ability to scale up due to aggregation. E-beam methods combined with 2-D plate were reported 4,5,14 . However, these methods only use a small area due to the limitation of the e-beam method and 2-D plate size, quantities of particles can be highly limited.…”

Section: Introductionmentioning

confidence: 99%

Synthesis method of asymmetric gold particles

Jun

Murata

Hahm

et al. 2017

Sci Rep

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Asymmetric particles can exhibit unique properties. However, reported synthesis methods for asymmetric particles hinder their application because these methods have a limited scale and lack the ability to afford particles of varied shapes. Herein, we report a novel synthetic method which has the potential to produce large quantities of asymmetric particles. Asymmetric rose-shaped gold particles were fabricated as a proof of concept experiment. First, silica nanoparticles (NPs) were bound to a hydrophobic micro-sized polymer containing 2-chlorotritylchloride linkers (2-CTC resin). Then, half-planar gold particles with rose-shaped and polyhedral structures were prepared on the silica particles on the 2-CTC resin. Particle size was controlled by the concentration of the gold source. The asymmetric particles were easily cleaved from the resin without aggregation. We confirmed that gold was grown on the silica NPs. This facile method for synthesizing asymmetric particles has great potential for materials science.

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“…57,58 The Au hemisphere was first functionalized with thiolated DNA, D1. The regioselective functionalization of JNP was observed when Au nanospheres (AuNSs) containing the complementary strand, D1′, selectively assembled only onto the Au side of the JNP but not the PS side.…”

Section: Using Dna For Spatial Control Of Nanoscale Assemblymentioning

confidence: 99%

DNA as a Powerful Tool for Morphology Control, Spatial Positioning, and Dynamic Assembly of Nanoparticles

Tan

Xing

Lu³

2014

Acc. Chem. Res.

212

152

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ConspectusSeveral properties of nanomaterials, such as morphologies (e.g., shapes and surface structures) and distance dependent properties (e.g., plasmonic and quantum confinement effects), make nanomaterials uniquely qualified as potential choices for future applications from catalysis to biomedicine. To realize the full potential of these nanomaterials, it is important to demonstrate fine control of the morphology of individual nanoparticles, as well as precise spatial control of the position, orientation, and distances between multiple nanoparticles. In addition, dynamic control of nanomaterial assembly in response to multiple stimuli, with minimal or no error, and the reversibility of the assemblies are also required. In this Account, we summarize recent progress of using DNA as a powerful programmable tool to realize the above goals. First, inspired by the discovery of genetic codes in biology, we have discovered DNA sequence combinations to control different morphologies of nanoparticles during their growth process and have shown that these effects are synergistic or competitive, depending on the sequence combination. The DNA, which guides the growth of the nanomaterial, is stable and retains its biorecognition ability. Second, by taking advantage of different reactivities of phosphorothioate and phosphodiester backbone, we have placed phosphorothioate at selective positions on different DNA nanostructures including DNA tetrahedrons. Bifunctional linkers have been used to conjugate phosphorothioate on one end and bind nanoparticles or proteins on the other end. In doing so, precise control of distances between two or more nanoparticles or proteins with nanometer resolution can be achieved. Furthermore, by developing facile methods to functionalize two hemispheres of Janus nanoparticles with two different DNA sequences regioselectively, we have demonstrated directional control of nanomaterial assembly, where DNA strands with specific hybridization serve as orthogonal linkers. Third, by using functional DNA that includes DNAzyme, aptamer, and aptazyme, dynamic control of assemblies of gold nanoparticles, quantum dots, carbon nanotubes, and iron oxide nanoparticles in response to one or more stimuli cooperatively have been achieved, resulting in colorimetric, fluorescent, electrochemical, and magnetic resonance signals for a wide range of targets, such as metal ions, small molecules, proteins, and intact cells. Fourth, by mimicking biology, we have employed DNAzymes as proofreading units to remove errors in nanoparticle assembly and further used DNAzyme cascade reactions to modify or repair DNA sequences involved in the assembly. Finally, by taking advantage of different affinities of biotin and desthiobiotin toward streptavidin, we have demonstrated reversible assembly of proteins on DNA origami.

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Bioinspired Nanocorals with Decoupled Cellular Targeting and Sensing Functionality

Cited by 131 publications

References 37 publications

SERS Tags: Novel Optical Nanoprobes for Bioanalysis

SERS Tags: Novel Optical Nanoprobes for Bioanalysis

Synthesis method of asymmetric gold particles

DNA as a Powerful Tool for Morphology Control, Spatial Positioning, and Dynamic Assembly of Nanoparticles

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