Bioinspired Multivalent Peptide Nanotubes for Sialic Acid Targeting and Imaging‐Guided Treatment of Metastatic Melanoma

Abstract

Tumor metastasis is considered a major cause of cancer‐related human mortalities. However, it still remains a formidable challenge in clinics. Herein, a bioinspired multivalent nanoplatform for the highly effective treatment of the metastatic melanoma is reported. The versatile nanoplatform is designed by integrating indocyanine green and a chemotherapeutic drug (7‐ethyl‐10‐hydroxycamptothecin) into phenylboronic acid (PBA)‐functionalized peptide nanotubes (termed as I/S‐PPNTs). I/S‐PPNTs precisely target tumor cells through multivalent interaction between PBA and overexpressed sialic acid on the tumor surface in order to achieve imaging‐guided combination therapy. It is demonstrated that I/S‐PPNTs are efficiently internalized by the B16‐F10 melanoma cells in vitro in a PBA grafting density–dependent manner. It is further shown that I/S‐PPNTs specifically accumulate and deeply penetrate into both the subcutaneous and lung metastatic B16‐F10 melanoma tumors. More importantly, I/S‐PPNT‐mediated combination chemo‐ and photodynamic therapy efficiently eradicates tumor and suppresses the lung metastasis of B16‐F10 melanoma in an immunocompetent C57BL/6 mouse model. The results highlight the promising potential of the multivalent peptide nanotubes for active tumor targeting and imaging‐guided cancer therapy.

Bioinspired Peptoid Nanotubes for Targeted Tumor Cell Imaging and Chemo‐Photodynamic Therapy

“…All these features promote peptoid‐based nanotubes as ideal nanocarriers for tumor intracellular imaging, drug delivery and cancer therapy. Unfortunately, the biomedical applications of 1D ONTs are still at the infant stage …”

Self-Assembled Nanorods of Phenylboronic Acid Functionalized Pyrene for In Situ Two-Photon Imaging of Cell Surface Sialic Acids and Photodynamic Therapy

“…As the concentration of Py-PBA NRs increased, the ARS ester emission at 600 nm gradually increased (Figure S12), suggesting the feasibility of ARS 1,2-diol recognition via boronic ester formation with the Py-PBA NRs. 46 When SA was added to the system, the fluorescence was further increased, indicating that SA was effectively combined with the Py-PBA NRs through the PBA-SA interaction to form a new intermediate. Meanwhile, FT-IR spectra also confirmed the reaction between the Py-PBA NRs and SA (Figure 2A).…”

“…With the incubation time extended to 1 h, Py-PBA NRs were slowly delivered into the cell cytoplasm, which might be ascribed to the SA mediated cellular internalization of Py-PBA NRs by endocytic pathways. 46 However, over half of all Py-PBA NRs (66.3% and 53.1%) still remained on the membrane after 6 h and 8 h (Figure S15). These results indicated that the Py-PBA NRs possessed excellent stability for the cell surface SA imaging.…”

Red Phosphorus Decorated TiO2 Nanorod Mediated Photodynamic and Photothermal Therapy for Renal Cell Carcinoma

“…[7] In recent years, therapeutics focused on killing cancer cells via nanomaterial-based phototoxicities, such as photodynamic therapy (PDT) and photothermal therapy (PTT), have been widely studied. [8,9] PDT is an effective clinical approach that uses a photoactivatable compound as a photosensitizer and irradiation with appropriate wavelength light. Under illumination, the photosensitizer can be activated as a generator of reactive oxygen species (ROS) to kill cancer cells efficiently.…”