Bioinspired mechanically stable all-polysaccharide based scaffold for photosynthetic production

Abstract

We demonstrate the construction of water-stable, biocompatible and self-standing hydrogels as scaffolds for the photosynthetic production of ethylene using a bioinspired all-polysaccharidic design combining TEMPO-oxidised cellulose nanofibers (TCNF) and a...

Employing photocurable biopolymers to engineer photosynthetic 3D-printed living materials for production of chemicals

“…Since the two tested light intensities (35 and 70 μmol photons m −2 s −1 ) resulted in comparable ethylene production yields, the data suggests that light saturation already occurs at 35 μmol photons m −2 s −1 under the studied conditions. For comparison, Synechocystis cells entrapped in Ca 2+ –Alg or Ca 2+ –PVA–TCNF films were reported to produce between 180 and 400 μmol mg Chl −1 ethylene under 35 μmol photons m −2 s −1 light, 17,39 which is noticeably lower than in our setup. The latter indicates a better biocatalytic performance of photocurable films under submerged cultivation.…”

“…6B), which was comparable to one observed in Ca 2+ –Alg, Ca 2+ –PVA–TCNF and Ca 2+ –MLG–TCNF films. 16,17,39 These results highlight the positive effect of the photo-crosslinked Alg + GGMMA + LAP matrix on the ethylene productivity of Synechocystis cells.…”