Bioinspired in vitro intestinal mucus model for 3D-dynamic culture of bacteria

Sardelli, Lorenzo; Briatico‐Vangosa, Francesco; Merli, Marta; Ziccarelli, Anna; Visentin, Sonja; Visai, Livia; Petrini, Paola

doi:10.1016/j.bioadv.2022.213022

Cited by 7 publications

(10 citation statements)

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“…[ 54 ] Recently, an in vitro mucus model for dynamic bacterial culture was reported. [ 55 ] Further, the role of the mucus layer was elucidated by mimicking host‐pathogen interactions in an in vitro model. [ 56 ] Generally, there is a trade‐off between efficiently synthesizing mucus with more accurate material properties and generating the correct mucin composition for the tissue being targeted.…”

Section: Current Methods Of Obtaining Mammalian Free‐flowing Mucus An...mentioning

confidence: 99%

Mucosa‐Mimetic Materials for the Study of Intestinal Homeostasis and Disease

Donahue

Sahoo

Rudolph

et al. 2023

Adv Healthcare Materials

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Mucus is a viscoelastic hydrogel that lines and protects the epithelial surfaces of the body that houses commensal microbiota and functions in host defense against pathogen invasion. As a first‐line physical and biochemical barrier, intestinal mucus is involved in immune surveillance and spatial organization of the microbiome, while dysfunction of the gut mucus barrier is implicated in several diseases. Mucus can be collected from a variety of mammalian sources for study, however, established methods are challenging in terms of scale and efficiency, as well as with regard to rheological similarity to native human mucus. Therefore, there is a need for mucus‐mimetic hydrogels that more accurately reflect the physical and chemical profile of the in vivo human epithelial environment to enable the investigation of the role of mucus in human disease and interactions with the intestinal microbiome. This review will evaluate the material properties of synthetic mucus mimics to date designed to address the above need, with a focus toward an improved understanding of the biochemical and immunological functions of these biopolymers related to utility for research and therapeutic applications.

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Section: Current Methods Of Obtaining Mammalian Free‐flowing Mucus An...mentioning

confidence: 99%

Mucosa‐Mimetic Materials for the Study of Intestinal Homeostasis and Disease

Donahue

Sahoo

Rudolph

et al. 2023

Adv Healthcare Materials

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“…In addition to the mucin-based approach discussed above, another synthetic approach to produce bioinspired intestinal mucus model was proposed by Sardelli et al in 2022. [321] They formed hydrogel (I-Bac3Gels) by mixing alginate with calcium carbonate and D-(+)-gluconic acid 𝛿-lactone to mimic the intestinal loose layer. The I-Bac3Gels exhibited a homogeneous distribution of E. coli (ATCC 25922) (Figure 12D), which qualitatively is similar to the results of in vivo animal studies.…”

Section: Mucus Modelsmentioning

confidence: 99%

“…in 2022. [ 321 ] They formed hydrogel (I‐Bac3Gels) by mixing alginate with calcium carbonate and D‐(+)‐gluconic acid δ‐lactone to mimic the intestinal loose layer. The I‐Bac3Gels exhibited a homogeneous distribution of E. coli (ATCC 25922) (Figure 12D), which qualitatively is similar to the results of in vivo animal studies.…”

Section: In Vitro Modelsmentioning

confidence: 99%

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In Vitro Models for Investigating Intestinal Host–Pathogen Interactions

McCoy,

Oldroyd,

Yang

et al. 2023

Advanced Science

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Infectious diseases are increasingly recognized as a major threat worldwide due to the rise of antimicrobial resistance and the emergence of novel pathogens. In vitro models that can adequately mimic in vivo gastrointestinal physiology are in high demand to elucidate mechanisms behind pathogen infectivity, and to aid the design of effective preventive and therapeutic interventions. There exists a trade‐off between simple and high throughput models and those that are more complex and physiologically relevant. The complexity of the model used shall be guided by the biological question to be addressed. This review provides an overview of the structure and function of the intestine and the models that are developed to emulate this. Conventional models are discussed in addition to emerging models which employ engineering principles to equip them with necessary advanced monitoring capabilities for intestinal host‐pathogen interrogation. Limitations of current models and future perspectives on the field are presented.

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“…The synergy with which the components of the "microbiota-mucus-epithelium" triad generates the final phenomenon of the leaky-gut is underestimated, independently from how the biological events are considered. It is therefore essential to optimize the traditional methods used for IBD studies -and in general mucosal-associated diseases -developing new technologies and tools that are able not only to the investigate the triad as a whole, but also to distinguish one by one the contribution that each actor performs towards the final result [11], [32]- [35] Figure 2 Graphical representation of molecular mechanisms involved in barrier impairment in a physiological (left) or IBD condition (right). (Left) SCFA-producing bacteria are abundant and release large quantity of SCFAs (green particles).…”

Section: General Aspect and Relevance Of Inflammatory Bowel Diseasementioning

confidence: 99%

Mucus Perspective to Conduct the Molecular Concertation in Inflammatory Bowel Diseases

Sardelli¹,

Boeri²,

Guagliano³

et al. 2023

Preprint

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The inflammatory bowels diseases (IBD) are autoimmune diseases that deeply impact the patients’ quality of life. The IBD pathogenesis is not yet defined, but evidence demonstrated that the IBD chronic inflammation is related to an impaired intestinal barrier. Traditionally, two actors were considered for their contribution to this disfunction: the gut microbiota and intestinal epithelium. However, a third element, which is the intestinal mucus, should be considered as peer of the epithelium and microbiota. Indeed, mucus represents the biological interface between bacteria and cells, filtering molecules or toxins and preventing bacteria penetration exploiting both structural and compositional properties. The boosting effect of the mucus characterization towards IBD comprehension is far too underestimated, although some mucus-oriented studies are already reported in literature. This work reviews the intestinal barrier features, describing each component of the gut mucosa (i.e., epithelium, microbiota, and mucus) in a mucus-oriented perspective.

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Bioinspired in vitro intestinal mucus model for 3D-dynamic culture of bacteria

Cited by 7 publications

References 74 publications

Mucosa‐Mimetic Materials for the Study of Intestinal Homeostasis and Disease

Mucosa‐Mimetic Materials for the Study of Intestinal Homeostasis and Disease

In Vitro Models for Investigating Intestinal Host–Pathogen Interactions

Mucus Perspective to Conduct the Molecular Concertation in Inflammatory Bowel Diseases

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