Bioinspired coupled helical coils for soft tissue engineering of tubular structures – Improved mechanical behavior of tubular collagen type I templates

Janke, Heinz P.; Bohlin, Jan; Lomme, Roger M. L. M.; Mihăilă, Silvia M.; Hilborn, Jöns; Feitz, W. F. J.; Oosterwijk, Egbert

doi:10.1016/j.actbio.2017.06.038

Cited by 12 publications

(6 citation statements)

References 42 publications

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“…For urological tissue engineering and specifically the artificial urinary conduit, the addition of a reinforcing polymer to the collagen, e.g., Vicryl, is desired to provide additional strength for template functionality and surgical handling. 25 , 28 Terminal sterilization of this hybrid tubular construct by EtO degassing or γ-irradiation did not lead to major differences between constructs with respect to the morphological and mechanical characteristics, similar to earlier studies for collagen alone. 29 Other studies already showed that γ-sterilization of collagen results in enhanced enzymatic degradation and reduced integrity and stability due to chain disintegration in collagen molecules in comparison to the nonsterilized construct.…”

Section: Discussionsupporting

confidence: 84%

“…Ureters from two different goats were similarly analyzed, spaced 1.27 mm apart ( n = 12). Data generation was performed as previously described. , In brief, recorded force–displacement data were normalized to the test specimen dimensions to compute a stress–strain curve. The stress was expressed as the recorded force F [N], which reacted on the cross-sectional area A [mm 2 ] of the test specimen (thickness ( t ) × width ( w ) × 2), while the strain was expressed as the change in length Δ L [mm] divided by its original length (hook-to-hook distance) L 0 [mm].…”

Section: Materials and Methodsmentioning

confidence: 99%

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The Impact of γ-Irradiation and EtO Degassing on Tissue Remodeling of Collagen-based Hybrid Tubular Templates

Sloff

Janke

Jonge

et al. 2018

ACS Biomater. Sci. Eng.

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Clinical implementation of novel products for tissue engineering and regenerative medicine requires a validated sterilization method. In this study, we investigated the effect of γ-irradiation and EtO degassing on material characteristics in vitro and the effect on template remodeling of hybrid tubular constructs in a large animal model. Hybrid tubular templates were prepared from type I collagen and Vicryl polymers and sterilized by 25 kGray of γ-irradiation or EtO degassing. The in vitro characteristics were extensively studied, including tensile strength analysis and degradation studies. For in vivo evaluation, constructs were subcutaneously implanted in goats for 1 month to form vascularized neo-tissue. Macroscopic and microscopic appearances of the γ- and EtO-sterilized constructs slightly differed due to additional processing required for the COL-Vicryl-EtO constructs. Regardless of the sterilization method, incubation in urine resulted in fast degradation of the Vicryl polymer and decreased strength (<7 days). Incubation in SBF was less invasive, and strength was maintained for at least 14 days. The difference between the two sterilization methods was otherwise limited. In contrast, subcutaneous implantation showed that the effect of sterilization was considerable. A well-vascularized tube was formed in both cases, but the γ-irradiated construct showed an organized architecture of vasculature and was mechanically more comparable to the native ureter. Moreover, the γ-irradiated construct showed advanced tissue remodeling as shown by enhanced ECM production. This study shows that the effect of sterilization on tissue remodeling cannot be predicted by in vitro analyses alone. Thus, validated sterilization methods should be incorporated early in the development of tissue engineered products, and this requires both in vitro and in vivo analyses.

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Section: Discussionsupporting

confidence: 84%

Section: Materials and Methodsmentioning

confidence: 99%

The Impact of γ-Irradiation and EtO Degassing on Tissue Remodeling of Collagen-based Hybrid Tubular Templates

Sloff

Janke

Jonge

et al. 2018

ACS Biomater. Sci. Eng.

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“…Graft was seeded with bone marrow‐derived mononuclear cells under vacuum condition and preclinical tested in sheep model (Best et al, ). Janke et al () also designed spiral collagen ring tubular graft to enhance the mechanical strength of the bioengineered tracheal transplant.…”

Section: Progress In Tracheal Tissue Engineeringmentioning

confidence: 99%

Biomedical grafts for tracheal tissue repairing and regeneration “Tracheal tissue engineering: an overview”

Dhasmana

Singh

2020

J Tissue Eng Regen Med

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Airway system is a vital part of the living being body. Trachea is the upper respiratory portion that connects nostril and lungs and has multiple functions such as breathing and entrapment of dust/pathogen particles. Tracheal reconstruction by artificial prosthesis, stents, and grafts are performed clinically for the repairing of damaged tissue. Although these (above-mentioned) methods repair the damaged parts, they have limited applicability like small area wounds and lack of functional tissue regeneration. Tissue engineering helps to overcome the abovementioned problems by modifying the traditional used stents and grafts, not only repair but also regenerate the damaged area to functional tissue. Bioengineered tracheal replacements are biocompatible, nontoxic, porous, and having 3D biomimetic ultrastructure with good mechanical strength, which results in faster and better tissue regeneration. Till date, the bioengineered tracheal replacements studies have been going on preclinical and clinical levels. Besides that, still many researchers are working at advance level to make extracellular matrix-based acellular, 3D printed, cell-seeded grafts including living cells to overcome the demand of tissue or organ and making the ready to use tracheal reconstructs for clinical application. Thus, in this review, we summarized the tracheal tissue engineering aspects and their outcomes. K E Y W O R D S biocompatible, chondrocytes, ECM, in vivo, tissue engineering, trachea

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“…Similarly, Janke H.P. et al ( 33 ) demonstrate a significant change in the mechanical properties of a collagen matrix reinforced with a double polymer spiral compared to an unreinforced one ( 33 ). The modulus of elasticity increased significantly.…”

Section: Discussionmentioning

confidence: 93%

Enhancing decellularized vascular scaffolds with PVDF and PCL reinforcement: a fused deposition modeling approach

Klyshnikov,

Rezvova,

Belikov

et al. 2023

Front. Cardiovasc. Med.

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BackgroundDecellularized xenogenic scaffolds represent a promising substrate for tissue-engineered vascular prostheses, particularly those with smaller diameters (<6 mm). Despite their benefits, a notable limitation presents itself during decellularization, namely, the diminished mechanical strength that introduces the risk of aneurysmal dilations in the early post-implantation period. This study introduces a strategy for modification the mechanical properties of these biological scaffolds through the forming of an external polymeric reinforcement via thermal extrusion.MethodsThe study utilized scaffolds fabricated from bovine internal mammary arteries through decellularization and preservation. The scaffolds were divided into subgroups and reinforced with polymeric helices made of Polyvinylidene fluoride (PVDF) and Polycaprolactone (PCL), n = 5 for each. An experimental setup for external reinforcement coating was designed. Computed microtomography was employed to obtain accurate 3D models of the scaffolds. Mechanical properties were evaluated through in vitro uniaxial tension tests (Z50, Zwick/Roell, Germany), compliance evaluation and numerical simulations (Abaqus/CAE, Dassault Systemes, France) to investigate the effect of external reinforcement on aneurysm growth.ResultsUsing a double-layer helix for the reinforcement significantly enhanced the radial tensile strength of the scaffolds, increasing it up to 2.26 times. Yet, the comparison of vessel's compliance between two reinforced and the Control scaffolds within the physiological pressures range did not reveal any significant differences. Numerical simulation of aneurysm growth showed that thin-walled regions of the Control scaffold developed aneurysmal-type protrusions, bulging up to 0.7 mm, with a substantial degradation of mechanical properties. In contrast, both PVDF and PCL reinforced scaffolds did not exhibit significant property degradation, with deformations ranging 0.1–0.13 mm depending on the model, and a maximum decrease in the modulus of elasticity of 23%.ConclusionThe results of the study demonstrated that the external polymer helical reinforcement of decellularized scaffolds via thermal extrusion enables a controlled modification of mechanical properties, notably enhancing radial strength while maintaining sufficient compliance within the physiological pressure range. A series of in vitro tests demonstrated the consistency and potential of this approach for decellularized xenogenic scaffolds, a concept that had not been explored before.

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Bioinspired coupled helical coils for soft tissue engineering of tubular structures – Improved mechanical behavior of tubular collagen type I templates

Cited by 12 publications

References 42 publications

The Impact of γ-Irradiation and EtO Degassing on Tissue Remodeling of Collagen-based Hybrid Tubular Templates

The Impact of γ-Irradiation and EtO Degassing on Tissue Remodeling of Collagen-based Hybrid Tubular Templates

Biomedical grafts for tracheal tissue repairing and regeneration “Tracheal tissue engineering: an overview”

Enhancing decellularized vascular scaffolds with PVDF and PCL reinforcement: a fused deposition modeling approach

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