Bioinspired construction of multi-enzyme catalytic systems

Shi, Jiafu; Wu, Yizhou; Zhang, Shaohua; Tian, Yu; Yang, Dong; Jiang, Zhongyi

doi:10.1039/c7cs00914c

Cited by 157 publications

(112 citation statements)

References 47 publications

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“…The majority of experimental investigations on hybrid lipid-copolymer vesicle are of hybrid GUVs for the convenience in characterization, since fluorescent-labeled microsized domains can be observed directly under fluorescent microscopy. [83,84,[87][88][89] In comparison, studies on hybrid LUVs are inadequate owing to the difficulty in characterizing nanosized and even smaller domains, typically techniques such as small-angle neutron scattering (SANS), time-resolved Förster resonance energy transfer (TR-FRET) and cryo-transmission electron microscopy (Cryo-TEM) are employed. [179,180] From systematic experimental studies, it was found that the difference in molecular weight/structure, the chemical compatibility between hydrophobic segments, and the molar fraction of lipids and copolymers in hybrid vesicles could be important for thermodynamic considerations.…”

Section: Hybrid Vesicles Asymmetric Vesiclesmentioning

confidence: 99%

“…For a prosperous and interdisciplinary field like synthetic cells, there are reviews summarizing from specific aspects, for instance, tailoring appearance of synthetic cells, [2,[70][71][72] elucidating specific functions of synthetic cell modules, [2,71,73] which include adhesion, [58] growth, [57] energy regeneration/conversion, [4,[59][60][61] gene circuits, [56,74] and cellular interactions, [75][76][77] exclusively focusing on applications of synthetic compartments such as delivery carriers [10,18,[78][79][80][81][82][83][84][85][86][22][23][24][25]42,47,48,55] and micro-/nanoreactors. [3,5,39,[87][88][89] However, there are no adequate state-of-art reviews with multidisciplinary perspective summarizing from the soft matter fundamentals to biomedical applications of synthetic compartments.…”

Section: Introductionmentioning

confidence: 99%

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Interface Engineering in Multiphase Systems toward Synthetic Cells and Organelles: From Soft Matter Fundamentals to Biomedical Applications

et al. 2020

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products of evolution over billions of years. They are featured by multiple hierarchical compartments performing specialized and exquisitely coordinated functions. The biological and genetic complexity of cells and subcellular components make understanding their physicochemical foundation piece by piece challenging. [1-5] Moreover, the mystery of how the very first cell, protocell, comes into exist in early earth scenario is unveiled yet. [6] Motivated by understanding protocell and biological cells, synthetic cells are being constructed. Started form the simplest form, aqueous droplet enclosed by a bilayer structure, researchers adopt a bottom-up approach to study machineries of biological cells, and explore possible forms of the protocell in early world conditions. [7] Synthetic cells are important to bridge the gap between cellular organism and nonliving matter. They refer to synthetic compartments that encapsulate a wide variety of molecules and mimic one or multiple cellular functions. By segregation of contents in different compartments, synthetic cells are now engineered to perform multiple processes and functions, including segregating genetic information, genetic circuits, protein synthesis, metabolism, growth, reproduce, recognition, intercellular crosstalk, and adaptivity to surroundings. [8-17] In these simplified cell models, cellular processes and signal pathways are reconstituted, providing insights for cell biology and offering new opportunities for designing smart cell-like carriers of therapeutics. [18-26] In addition, the hypothesis of "RNA world" has inspired the investigation of synthetic compartments as protocells, in which nucleotide permeation, compartmental division, the synthesis of macromolecular polynucleotide, and the polymerization of ribonucleic acids (RNA) are explored. [7,27,28] The beginning of synthesizing cell-like structures starts from amphiphiles self-assembled at liquid/liquid interfaces to form enclosed compartments. [29-32] Recently, techniques such as microfluidics facilitate the fabrication of complex compartments with high-order hierarchy with excellent control. [33-36] Formulations of compartmentalization can be diverse, there are reviews mainly focused on one particular type of synthetic compartments, such as lipid vesicles (liposomes), [22,30,37,38] polymer vesicles (polymersomes), [39-43] lipid-polymer hybrid Synthetic cells have a major role in gaining insight into the complex biological processes of living cells; they also give rise to a range of emerging applications from gene delivery to enzymatic nanoreactors. Living cells rely on compartmentalization to orchestrate reaction networks for specialized and coordinated functions. Principally, the compartmentalization has been an essential engineering theme in constructing cell-mimicking systems. Here, efforts to engineer liquid-liquid interfaces of multiphase systems into membrane-bounded and membraneless compartments, which include lipid vesicles, polymer vesicles, colloidosomes, hybrids, and coacervate droplets,...

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Section: Hybrid Vesicles Asymmetric Vesiclesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interface Engineering in Multiphase Systems toward Synthetic Cells and Organelles: From Soft Matter Fundamentals to Biomedical Applications

et al. 2020

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“…Experimentally, this question is raised by synthetic biology e↵orts that use various scaffolding strategies to construct e cient multi-enzyme complexes [48][49][50], inspired by natural complexes consisting of multiple enzymes in intricate arrangements [51]. Here, we explore this question from a theoretical perspective, starting with the idealized scenario of spherical catalysts that can be freely arranged into complexes with any geometry: how should the catalysts be arranged to globally maximize the pathway flux j P ?…”

Section: Geometries Of Optimal Complexesmentioning

confidence: 99%

Trade-offs and design principles in the spatial organization of catalytic particles

Hinzpeter¹,

Tostevin²,

Büchner³

et al. 2020

Preprint

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Spatial organization of catalytic particles is ubiquitous in biological systems across di↵erent length scales, from enzyme complexes to metabolically coupled cells. Despite the di↵erent scales, these systems share common features of localized reactions with partially hindered di↵usive transport, determined by the collective arrangement of the catalysts. Yet it remains largely unexplored how di↵erent arrangements a↵ect the interplay between the reaction and transport dynamics, which ultimately determines the flux through the reaction pathway. Here we show that two fundamental trade-o↵s arise, the first between e cient inter-catalyst transport and depletion of substrate, and the second between steric confinement of intermediate products and accessibility of catalysts to substrate. We use a model reaction pathway to characterize the general design principles for the arrangement of catalysts that emerge from the interplay of these trade-o↵s. We find that the question of optimal catalyst arrangements generalizes the famous Thomson problem of electrostatics.

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“…In biological systems, enzyme or protein analytical sensing system could combine with the specific recognition site or binding affinity as a sensing system to design the analytical platform [64] Using the substrate DNA as an efficient template for determining nuclease or protein activity has many advantages, such as simplicity, rapidness, sensitivity, and userfriendly. [65] An assay has been designed to detect the 3'-5' exonuclease activity by using dsDNA-templated Cu-NPs.…”

Section: Bioassay For Enzyme Protein and Exosomes Analysismentioning

confidence: 99%

DNA‐Templated Copper Nanoprobes: Overview, Feature, Application, and Current Development in Detection Technologies

Chen

Shiddiky

et al. 2019

The Chemical Record

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Metal nanoprobes have recently attracted board research interestinr their application in establishing sensing systems due to their unique optical, electrical, physical, and chemical properties. In comparison to gold and silver nanoprobes, analytical platform based on copper nanoprobes (Cu‐NPs) is still in the early stages of development. In this review, we focus on single‐stranded, and double‐stranded DNA capped Cu‐NPs sensing systems which have been designed for various analytes, including metal ions, anions, small molecules, biomolecules (DNA, RNA, and protein, etc.). In addition, the application of Cu‐NPs in biological labeling or bio‐imaging platforms has also been introduced and summarized.

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Bioinspired construction of multi-enzyme catalytic systems

Cited by 157 publications

References 47 publications

Interface Engineering in Multiphase Systems toward Synthetic Cells and Organelles: From Soft Matter Fundamentals to Biomedical Applications

Interface Engineering in Multiphase Systems toward Synthetic Cells and Organelles: From Soft Matter Fundamentals to Biomedical Applications

Trade-offs and design principles in the spatial organization of catalytic particles

DNA‐Templated Copper Nanoprobes: Overview, Feature, Application, and Current Development in Detection Technologies

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