Bioinsecticide production by the bacterium<i>Bacillus thuringiensis</i>. 1. Pattern of cell growth, toxin production and by-product synthesis

Amin, G.; AlOtaibi, Saad Aied; Narmen, Youssef A.; Saleh, W.

doi:10.1080/03650340802158201

Cited by 11 publications

(4 citation statements)

References 11 publications

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“…Similarly, several secondary metabolites were reported as nongrowth associated products of microorganisms. Among them, the crystal protein toxin produced by the bacterium Bacillus thuringiensis (Amin et al 2008a), and the nontoxic red pigment produced by the fungus Monascus spp. (Juslová et al 1996;Hamano et al 2005).…”

Section: Resultsmentioning

confidence: 99%

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Assessment of insecticidal activity of red pigment produced by the fungus Beauveria bassiana

Amin

Youssef

Bazaid

et al. 2010

World J Microbiol Biotechnol

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Insecticidal activity of the red pigment produced by a strain of the fungus Beauveria bassiana that was locally isolated from infected whitefly, Bemisia tabaci (Genn.) was assessed. The pigment is produced extracellularly and is a water-soluble. This makes it easy and simple to be recovered from fermentation broth and used in pathogenicity experiments. When applied alone to Bm. tabaci nymphs, mortality percentages of 18% was recorded. For nymphs treated with Bv. bassiana spore suspension, mortality was 60%. The best results were obtained when red pigment was combined with fungal spores with the mortality percentage being increased to up to 92%. The highest insecticidal activity against adults emerging later on from the surviving larvae of Bm. tabaci was recorded also with treatment combining pigment and fungal spores with the longest days to pupation.

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Section: Resultsmentioning

confidence: 99%

“…Then, they were left to dry at room temperature for 10 days. At 2-day regular intervals, leaves were examined under binocular stereo-microscope where dead and living nymphs were counted in order to calculate percentages of mortality which were corrected by Abbot's formula (Amin et al 2008a). …”

Section: Toxicitymentioning

confidence: 99%

Assessment of insecticidal activity of red pigment produced by the fungus Beauveria bassiana

Amin

Youssef

Bazaid

et al. 2010

World J Microbiol Biotechnol

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“…Nutrition content highly influenced the process of cell and spore production of B. thuringiensis (Purnawati et al, 2014). Furthermore, it was stated that the concentration of nutritional components in the growth media of B. thuringiensis, such as glucose and mineral salts, could increase the spore production (Amin et al, 2008;Sarrafzadeh, 2012). The development of B. thuringiensis cells was better when the nutritional content of the growth media was more complete, including vitamins and minerals (Paul et al, 2011).…”

Section: Mortality=mentioning

confidence: 99%

The Use of Golden Snail Meal to Enrich Bacillus Thuringiensis Culture Media and Its Effect on the Bacterial Toxicity Against Spodoptera Litura

Pujiastuti

Masyitah²,

Dirgahayu³

et al. 2018

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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The use of golden snail meal to enrich Bacillus thuringiensis culture media and its effect on the bacterial toxicity against spodoptera litura. Bacillus thuringiensis is an entomopathogenic bacterium producing spore and protein at sporulation. There has been limited research on using golden snail as protein resource to enrich bacterial culture media. This research was aimed at studying bacterial cell/spore production in culture media made from coconut water media and liquid waste of tofu industry (tofu whey) enriched with golden snail meal (GSM), as well as its toxicity against Spodoptera litura. The research was conducted in the Laboratory of Entomology, Department of Plant Protection, Faculty of Agriculture, Sriwijaya University, from June to September 2017. The experiment was arranged in a Factorial Completely Randomized Design (FCRD) with two factors and three replications. The first factor was the addition of GSM (0, 5, 9, 13 and 17 g) and the second was storage longevity (0, 1, and 2 months). Number of test insect (third instar of S. litura) was 30 larvae per treatment. Parameter observed were spore density, larval mortality, and symptom of infected larvae. The results showed that B. thuringiensis cultured in culture media enriched with 13 g golden snail meal produced the highest spore density, amounted to 14.14 x 107spores/ml and caused larval mortality up to 86.67%. After one and two month storage treatments, the spore density in the cultures reduced to 2.51 x 107 and 1.49 x 107 spores/ml, respectively. There was a tendency of reduction in spore density under longer storage of the culture. Survived larvae developed abnormally, failed to transform to pupa or imago.

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“…The ingredients in the media affect the rate and synthesis of the different δ-endotoxins and also the size of the crystals produced. Using barley as the carbon source, Amin [79] developed a cost-effectively protocol for the mass production of Bt.…”

Section: Production Media and Media Formulationsmentioning

confidence: 99%

An Overview on the Crystal Toxins from <i>Bacillus thuringiensis</i>

Jisha¹,

Smitha²,

Benjamin³

2013

AiM

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Strains of Bacillus thuringiensis (Bt) are known to produce crystalline proteins (δ-endotoxins) concomitantly with sporulation during their stationary phase of growth, which are demonstrated as lethal to lepidopeterous, coleopeterous and dipterous insects in addition to mites, nematodes, protozoa and flukes. Upon ingestion, the δ-nascent endotoxin is an inactive protoxin complex of (Cry alone or Cry and Cyt toxins together) high molecular mass, which is cleaved upon ingestion into the active component proteins at the high alkaline environments in the digestive tract of these agricultural pests. Conventionally, Bt-crystals are being produced employing submerged or liquid fermentation techniques in commercial media, but recently many workers have used solid-state fermentation strategy for the enhanced production of Bt-toxin at low cost. Apart from δ-endotoxin, some isolates of Bt produce another class of insecticidal small molecules called β-exotoxin (thuringiensin), which may be harmful to humans. Moreover, resistance to Bt developed in various target pest is yet another concern for Bt-industry. Following a brief introduction, this review addresses various toxins produced by various strains of Bt, Bt production media and media formulations with emphasis to solid-state fermentation, general structure of Cry toxin, its mode of action, target pests, bioassay, resistance to Bt toxins and resistance management. Briefly, this review would provide the readers an overview on the general aspects of Bt toxin, its general structure and mechanism of action.

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Bioinsecticide production by the bacteriumBacillus thuringiensis. 1. Pattern of cell growth, toxin production and by-product synthesis

Cited by 11 publications

References 11 publications

Assessment of insecticidal activity of red pigment produced by the fungus Beauveria bassiana

Assessment of insecticidal activity of red pigment produced by the fungus Beauveria bassiana

The Use of Golden Snail Meal to Enrich Bacillus Thuringiensis Culture Media and Its Effect on the Bacterial Toxicity Against Spodoptera Litura

An Overview on the Crystal Toxins from <i>Bacillus thuringiensis</i>

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Bioinsecticide production by the bacteriumBacillus thuringiensis. 1. Pattern of cell growth, toxin production and by-product synthesis

Cited by 11 publications

References 11 publications

Assessment of insecticidal activity of red pigment produced by the fungus Beauveria bassiana

Assessment of insecticidal activity of red pigment produced by the fungus Beauveria bassiana

The Use of Golden Snail Meal to Enrich Bacillus Thuringiensis Culture Media and Its Effect on the Bacterial Toxicity Against Spodoptera Litura

An Overview on the Crystal Toxins from &lt;i&gt;Bacillus thuringiensis&lt;/i&gt;

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An Overview on the Crystal Toxins from <i>Bacillus thuringiensis</i>