Bioinformatics analysis of the expression of HOXC13 and its role in the prognosis of breast cancer

Abstract

The homeobox (HOX) genes, a class of transcription factors, are known to promote embryonic development and induce tumor formation. To date, the HOXA and HOXB gene families have been reported to be associated with breast cancer. However, the expression and exact role of homeobox C13 (HOXC13) in breast cancer has not yet been investigated. In the present study, the HOXC13 expression in human breast cancer was evaluated using the Oncomine database and Cancer Cell Line Encyclopedia (CCLE). Next, the Gene expression-based Outcome for Breast cancer online database, cBioportal, University of California Santa Cruz Xena browser and bc-GenExMinerv were used to explore the specific expression of HOXC13 in breast cancer. The methylation and mutation status of HOXC13 in breast cancer was then validated using the CCLE and cBioportal databases. Finally, the co-expression of HOX transcript antisense RNA (HOTAIR) and HOXC13 in breast cancer were analyzed and their impact on clinical prognosis determined. It was found that the expression of HOXC13 was high in breast cancer compared with other types of cancer, such as gastric cancer and colon cancer. Following co-expression analysis, a significant positive association was identified between HOTAIR and HOXC13. An association between HOTAIR and HOXC13, and lymph node and distant metastasis recurrence was also revealed during the development of breast cancer. Of note, survival analysis showed that high expression of HOTAIR and HOXC13 predicted poor prognosis. These findings revealed that HOXC13 plays an important role in the progression of breast cancer. However, the specific mechanism needs to be confirmed by subsequent experiments.

Analysis of H3K4me3-ChIP-Seq and RNA-Seq data to understand the putative role of miRNAs and their target genes in breast cancer cell lines

“…13). In normal-like (MCF10A) vs. TNBC (MB231 and MB436) downregulated genes, 15 genes have been predicted in this study and their role in breast cancer have also been previously reported (TNFSF10, TMEM47, IQGAP2, FAT4, NUPR1, HOXC13, PRRX1, STC2, AC108941.2, ADAMTSL1, ARHGEF5, BNC1, CPA4, PPL, and TNFRSF10D) [52][53][54][55][56][57][58][59][60][61][62][63][64][65][66] (Fig. 5, Supplementary Ta-bles 6, 20, Supplementary Fig.…”

LINC00659 Regulates Colorectal Cancer Migration and Invasion by Targeting miR-485-5p/HOXC13 Axis

“…HOXC13 is a member of the homeobox family, which binds to a speci c nucleotide sequence on DNA and activates or inhibits the transcription process [36][37][38].It has been reported that high expression of HOX proteins may lead to carcinogenesis [39]. The expression of HOXC13 was reported to be upregulated in several kinds of cancers, including esophageal squamous cell carcinoma, lung adenocarcinoma, breast cancer and melanoma [40][41][42][43]. Especially, aberrant expression of HOXC13 was also associated with colorectal cancer [44,45] and knockdown of HOXC12 could signi cantly inhibit colorectal cancer cell growth and result in cell cycle arrest [46].…”

An in silico approach to identify and prioritize miRNAs target sites polymorphisms in colorectal cancer and obesity

“…The ADCY9 expression remarkably increased in colon tumor tissue and can be a poor prognostic factor for colon survival 42 . The HOXC13 and CSNK2B expression increase in breast cancer and play a key role in the progression of breast cancer 43,44 . The expression of cyclins regulated by HOXC13 and knockdown of this gene resulted to cell cycle arrest and apoptosis, in the colon cancer cell lines 45 .…”

“…42 The HOXC13 and CSNK2B expression increase in breast cancer and play a key role in the progression of breast cancer. 43,44 The expression of cyclins regulated by HOXC13 and knockdown of this gene resulted to cell cycle arrest and apoptosis, in the colon cancer cell lines. 45 The roles of identified miRNAs in cancer were also determined.…”