Bioinformatics analysis of evolutionary characteristics and biochemical structure of FGF5 Gene in sheep

Zhang, Rui; Wu, Hongping; Lian, Zhengxing

doi:10.1016/j.gene.2019.03.040

Cited by 21 publications

(15 citation statements)

References 45 publications

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“…Another publication has also reported the generation of FGF5 -disrupted sheep; 16/20 (80%) mutated founders carried both monoallelic and biallelic mutations in FGF5 and showed increased wool length and quantity (Li et al, 2017c). Recently, Zhang et al have also confirmed that the disruption of FGF5 in sheep can lead to an increased wool length and average wool growth rate (Zhang et al, 2019c). The results of these studies confirm the functional role of FGF5 and its desired disrupting effect.…”

Section: Applications Of Crispr/cas9 In Sheep and Goatsmentioning

confidence: 89%

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

et al. 2019

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Sheep and goats are valuable livestock species that have been raised for their production of meat, milk, fiber, and other by-products. Due to their suitable size, short gestation period, and abundant secretion of milk, sheep and goats have become important model animals in agricultural, pharmaceutical, and biomedical research. Genome engineering has been widely applied to sheep and goat research. Pronuclear injection and somatic cell nuclear transfer represent the two primary procedures for the generation of genetically modified sheep and goats. Further assisted tools have emerged to enhance the efficiency of genetic modification and to simplify the generation of genetically modified founders. These tools include sperm-mediated gene transfer, viral vectors, RNA interference, recombinases, transposons, and endonucleases. Of these tools, the four classes of site-specific endonucleases (meganucleases, ZFNs, TALENs, and CRISPRs) have attracted wide attention due to their DNA double-strand break-inducing role, which enable desired DNA modifications based on the stimulation of native cellular DNA repair mechanisms. Currently, CRISPR systems dominate the field of genome editing. Gene-edited sheep and goats, generated using these tools, provide valuable models for investigations on gene functions, improving animal breeding, producing pharmaceuticals in milk, improving animal disease resistance, recapitulating human diseases, and providing hosts for the growth of human organs. In addition, more promising derivative tools of CRISPR systems have emerged such as base editors which enable the induction of single-base alterations without any requirements for homology-directed repair or DNA donor. These precise editors are helpful for revealing desirable phenotypes and correcting genetic diseases controlled by single bases. This review highlights the advances of genome engineering in sheep and goats over the past four decades with particular emphasis on the application of CRISPR/Cas9 systems.

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Section: Applications Of Crispr/cas9 In Sheep and Goatsmentioning

confidence: 89%

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

et al. 2019

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“…Candidate genes related to meat and wool traits. Genes associated with meat quality, including CAPN3 34,36,38 , SOX6 [72][73][74][75][76] , FGF5 [80][81][82][83] , FGF7 and VEPH1 84,85 , were identified in several candidate genomic regions under selection. Calpain 3 (Capn3), a skeletal muscle-specific member of the calpain family, was suggested to be related with muscle growth in cattle 36,71,77,86 and chicken 34,87 .…”

Section: Linkage Disequilibrium (Ld) Analysis Runs Of Homozygosity (Roh) and Pairwise Sequentially Markovian Coalescent (Psmc)mentioning

confidence: 99%

“…Calpain 3 (Capn3), a skeletal muscle-specific member of the calpain family, was suggested to be related with muscle growth in cattle 36,71,77,86 and chicken 34,87 . Fibroblast growth factor (FGF) 5 regulates the development and periodicity of hair follicles, which is related to wool or cashmere growth in cats, sheep and goats 80,81,83 . FGF5 knockout sheep or goats have significantly increased hair follicles, fiber length and growth rates 80 .…”

Section: Linkage Disequilibrium (Ld) Analysis Runs Of Homozygosity (Roh) and Pairwise Sequentially Markovian Coalescent (Psmc)mentioning

confidence: 99%

Genome-wide comparative analyses reveal selection signatures underlying adaptation and production in Tibetan and Poll Dorset sheep

Zhang

Xue

Liu

et al. 2021

Sci Rep

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The identification of genome-wide selection signatures can provide insights on the mechanisms of natural and/or artificial selection and uncover genes related to biological functions and/or phenotypes. Tibetan sheep are an important livestock in Tibet, providing meat and wool for Tibetans who are renown for breeding livestock that adapt well to high altitudes. Using whole-genome sequences with an effective sequencing depth of 5×, we investigated the genomic diversity and structure and, identified selection signatures of White Tibetan, Oula and Poll Dorset sheep. We obtained 30,163,679 Single Nucleotide Polymorphisms (SNPs) and 5,388,372 indels benchmarked against the ovine Oar_v4.0 genome assembly. Next, using FST, ZHp and XP-EHH approaches, we identified selection signatures spanning a set of candidate genes, including HIF1A, CAPN3, PRKAA1, RXFP2, TRHR and HOXA10 that are associated with pathways and GO categories putatively related to hypoxia responses, meat traits and disease resistance. Candidate genes and GO terms associated with coat color were also identified. Finally, quantification of blood physiological parameters, revealed higher levels of mean corpuscular hemoglobin measurement and mean corpuscular hemoglobin concentration in Tibetan sheep compared with Poll Dorset, suggesting a greater oxygen-carrying capacity in the Tibetan sheep and thus better adaptation to high-altitude hypoxia. In conclusion, this study provides a greater understanding of genome diversity and variations associated with adaptive and production traits in sheep.

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“…Similarly, the FGF gene family had also been classified into eight subfamilies; wherein, the FGF5 genes were placed in a subfamily comprising FGF4, FGF5 , and FGF6, while FGF3 was classified into one independent clade (Oulion et al., 2012). Additionally, FGF genes were classified into seven subfamilies in a recent study, which classified FGF3 genes into a subfamily containing FGF3 , FGF7, FGF10 , and FGF22 , while FGF4, FGF5 , and FGF6 were placed in one single subfamily (Zhang et al., 2019). However, FGF5 was placed in FGF1 subfamily according to synteny analyses (Itoh & Ornitz, 2008).…”

Section: Discussionmentioning

confidence: 99%

FGF gene family characterization provides insights into its adaptive evolution in Carnivora

Wei

Dong

Sun

et al. 2021

Ecology and Evolution

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Fibroblast growth factors (FGFs), which participate in diverse biological activities, such as lipid and carbohydrate metabolism, play vital roles in animal development and physiology. They are a family of very conservative growth factors and comprise 22 members in mammals (Brewer et al., 2016). The 22 FGFs can be divided into three types: the canonical FGFs (cFGFs), the hormone-like FGFs (hFGFs), and the intracellular FGFs (iFGFs) (Itoh & Ornitz, 2011). While cFGFs and hFGFs are ligands that act through binding to appropriate receptors, iFGFs can act independently of receptor-binding.The hFGFs contained members of FGF19, FGF21, and FGF23; the iFGFs include FGF11, FGF12, FGF13, and FGF14; the rest members (FGF1 to FGF10, FGF16, FGF 17, FGF18, FGF20, and FGF22) constitute the cFGFs (Ornitz & Itoh, 2015). The cFGFs play important roles in cell growth, differentiation, and organ development and

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Bioinformatics analysis of evolutionary characteristics and biochemical structure of FGF5 Gene in sheep

Cited by 21 publications

References 45 publications

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

Genome-wide comparative analyses reveal selection signatures underlying adaptation and production in Tibetan and Poll Dorset sheep

FGF gene family characterization provides insights into its adaptive evolution in Carnivora

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