Bioinformatics analysis of candidate genes for milk production traits in water buffalo (Bubalus bubalis)

Du, Chao; Deng, Tingxian; Zhou, Yiwen; Ghanem, Nasser; Hua, Guo

doi:10.1007/s11250-019-01984-1

Cited by 13 publications

(11 citation statements)

References 25 publications

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“…In mice and humans, DGAT2 was found to express with the highest expression levels in the tissues of adipose tissue, liver, small intestine, and mammary gland, which are associated with TG synthesis (Cases et al, 2001). The study in yak showed that the DGAT2 was abundant mostly in subcutaneous fat, moderate in liver, heart, longissimus dorsi, and abomasum but lower in the large intestine, small intestine, mammary gland, lungs, kidney, spleen, and rumen (Hu et al, 2019). The DGAT2 gene was also found extensively expressed in adult pigs with the highest level in the liver (Yin et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Water buffalo (Bubalus bubalis), as an important livestock resource in tropical and subtropical countries, has made an important contribution to the agricultural production of meat and milk as well as certain forms of labor (Mokhber et al, 2018;Du et al, 2019). Water buffalo provides more than 5 % of the world's milk supply.…”

Section: Introductionmentioning

confidence: 99%

“…Water buffalo have leaner meat that contains less fat and cholesterol than beef while having a comparable taste (Michelizzi et al, 2010). Over the past 50 years, the total milk production of buffalo has increased significantly because its population is growing at an annual rate of 1.65 % (Du et al, 2019). There are two types of buffalo -river buffalo and swamp buffalowhich differ in chromosome number, morphology, and behavior (Nahas et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Identification, molecular characteristics, and tissue differential expression of DGAT2 full-CDS cDNA sequence in Binglangjiang buffalo (Bubalus bubalis)

et al. 2020

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Abstract. It has been found that diacylglycerol acyltransferase-2 (DGAT2) plays a crucial role in the synthesis of triglycerides (TGs) in some mammals, but its role in buffalo lactation is unclear. In the present study, the DGAT2 full-CDS cDNA sequence of Binglangjiang buffalo was isolated, and the physicochemical characteristics and structure of its encoding protein were characterized. Furthermore, the differential expressions of this gene in 10 tissues of lactating and non-lactating buffalo were analyzed by real-time quantitative PCR (RT-qPCR). The results showed that the coding region (CDS) of this gene was 1086 bp in length, encoding a peptide composed of 361 amino acid residues. The deduced amino acid sequence shared more than 98.6 % identity with that of cattle, zebu, yak, and bison in the Bovidae family. Buffalo DGAT2 protein is a slightly hydrophobic protein with a transmembrane region, which functions in membrane of endoplasmic reticulum. Besides, this protein belongs to the LPLAT_MGAT-like family and contains a conserved domain of DAGAT that has a function in the synthesis of TGs. The multi-tissue differential expression analysis demonstrated that DGAT2 was expressed in the heart, liver, mammary gland, and muscle in both non-lactating and lactating buffalo. And its expression level in the heart, liver, and mammary gland during lactation was significantly higher than that during non-lactation. The results indicate that buffalo DGAT2 may be involved in milk fat synthesis. This study can establish a foundation for further elucidating mechanisms of the buffalo DGAT2 gene in milk fat synthesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification, molecular characteristics, and tissue differential expression of DGAT2 full-CDS cDNA sequence in Binglangjiang buffalo (Bubalus bubalis)

et al. 2020

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“…Bioinformatics analysis provides useful information for understanding the biological and biochemical mechanisms of genes at the molecular level [ 23 ]. Recent studies reported highly reliable results by analyzing the pathway or protein-protein interaction (PPI) based on candidate genes associated with milk production in dairy cattle [ 24 , 25 , 26 , 27 , 28 ]. The aim of the present study was to identify chromosome regions and candidate genes related to four milk production traits—adjusted 305-day milk yield (MY), adjusted 305-day fat yield (FY), adjusted 305-day protein yield (PY), and somatic cell score (SCS)—in a Korean Holstein population using high-density SNP chip platforms and two models to perform GWAS and fine mapping.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Candidate Genes for Milk Production Traits in Korean Holstein Cattle

Kim

Lim

Cho³

et al. 2021

Animals

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We performed a genome-wide association study and fine mapping using two methods (single marker regression: frequentist approach and Bayesian C (BayesC): fitting selected single nucleotide polymorphisms (SNPs) in a Bayesian framework) through three high-density SNP chip platforms to analyze milk production phenotypes in Korean Holstein cattle (n = 2780). We identified four significant SNPs for each phenotype in the single marker regression model: AX-311625843 and AX-115099068 on Bos taurus autosome (BTA) 14 for milk yield (MY) and adjusted 305-d fat yield (FY), respectively, AX-428357234 on BTA 18 for adjusted 305-d protein yield (PY), and AX-185120896 on BTA 5 for somatic cell score (SCS). Using the BayesC model, we discovered significant 1-Mb window regions that harbored over 0.5% of the additive genetic variance effects for four milk production phenotypes. The concordant significant SNPs and 1-Mb window regions were characterized into quantitative trait loci (QTL). Among the QTL regions, we focused on a well-known gene (diacylglycerol O-acyltransferase 1 (DGAT1)) and newly identified genes (phosphodiesterase 4B (PDE4B), and anoctamin 2 (ANO2)) for MY and FY, and observed that DGAT1 is involved in glycerolipid metabolism, fat digestion and absorption, metabolic pathways, and retinol metabolism, and PDE4B is involved in cAMP signaling. Our findings suggest that the candidate genes in QTL are strongly related to physiological mechanisms related to the fat production and consequent total MY in Korean Holstein cattle.

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“…Zinc and certain other trace minerals have a variety of antioxidants that act as immune system boosters. [7] Studies have shown that Barley broth (Talbinah) is the food item by which mood and depression reduces. [8] Mood and cognitive function are influenced by macronutrients and micronutrients.…”

Section: Introductionmentioning

confidence: 99%

Nutritional and therapeutic properties of barley broth (Talbinah): recent updates

Afzaal

Saeed

Shahzadi

et al. 2021

International Journal of Food Properties

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Barley broth (Talbinah) is prepared by combining barley syrup, milk, and honey. The Prophet Mohammad (PBUH) recommended Talbinah for sadness and heart relief in sorrows. Talbinah has a promising nutritional and therapeutic impact on human health. It is a rich source of many nutrients and some bioactive moieties. Regular consumption imparts in maintaining better gut and acts as anti-depressive, anti-diabetic, anti-oxidant, anti-inflammatory, antidiabetic and antihypercholesterolemic. Moreover, it is the most effective food for supplying nutrients and removing toxins from human cells. In this review, the production technology, nutrition, therapeutic potential, proposed prospects, role as a functional food and current knowledge on the subject have been discussed comprehensively. Conclusively, Talbinah as functional food could be a way in preventing various health maladies.

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Bioinformatics analysis of candidate genes for milk production traits in water buffalo (Bubalus bubalis)

Cited by 13 publications

References 25 publications

Identification, molecular characteristics, and tissue differential expression of <i>DGAT2</i> full-CDS cDNA sequence in Binglangjiang buffalo (<i>Bubalus bubalis</i>)

Identification, molecular characteristics, and tissue differential expression of <i>DGAT2</i> full-CDS cDNA sequence in Binglangjiang buffalo (<i>Bubalus bubalis</i>)

Genome-Wide Identification of Candidate Genes for Milk Production Traits in Korean Holstein Cattle

Nutritional and therapeutic properties of barley broth (Talbinah): recent updates

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Bioinformatics analysis of candidate genes for milk production traits in water buffalo (Bubalus bubalis)

Cited by 13 publications

References 25 publications

Identification, molecular characteristics, and tissue differential expression of &lt;i&gt;DGAT2&lt;/i&gt; full-CDS cDNA sequence in Binglangjiang buffalo (&lt;i&gt;Bubalus bubalis&lt;/i&gt;)

Identification, molecular characteristics, and tissue differential expression of &lt;i&gt;DGAT2&lt;/i&gt; full-CDS cDNA sequence in Binglangjiang buffalo (&lt;i&gt;Bubalus bubalis&lt;/i&gt;)

Genome-Wide Identification of Candidate Genes for Milk Production Traits in Korean Holstein Cattle

Nutritional and therapeutic properties of barley broth (Talbinah): recent updates

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Identification, molecular characteristics, and tissue differential expression of <i>DGAT2</i> full-CDS cDNA sequence in Binglangjiang buffalo (<i>Bubalus bubalis</i>)

Identification, molecular characteristics, and tissue differential expression of <i>DGAT2</i> full-CDS cDNA sequence in Binglangjiang buffalo (<i>Bubalus bubalis</i>)