Bioinformatic identification and characterization of new members of short-chain dehydrogenase/reductase superfamily

Keller, Brigitte; Volkmann, Andrea; Wilckens, Thomas; Moeller, Gabriele; Adamski, Jerzy

doi:10.1016/j.mce.2005.10.025

Cited by 20 publications

(15 citation statements)

References 10 publications

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“…Haeseleer et al (13) found 17HSD14 expression at low levels in kidney and high levels in retina; no expression was detected in heart, brain, lung, or liver. On the contrary, Keller et al (17) found high levels in lung, kidney, liver, and spleen. In both studies, single samples were investigated with one method.…”

Section: Discussionmentioning

confidence: 91%

“…Moreover, in the multivariate analyses, the treatment effect was adjusted for. Expression of 17HSD14 has been examined previously by Northern blot in normal samples from several human tissues and cell types (13,17). Haeseleer et al (13) found 17HSD14 expression at low levels in kidney and high levels in retina; no expression was detected in heart, brain, lung, or liver.…”

Section: Discussionmentioning

confidence: 99%

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17β-Hydroxysteroid Dehydrogenase 14 Affects Estradiol Levels in Breast Cancer Cells and Is a Prognostic Marker in Estrogen Receptor–Positive Breast Cancer

Jansson¹,

Gunnarsson

Cohen³

et al. 2006

Cancer Research

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Estrogens have an important role in the progression of breast cancer. The 17B-hydroxysteroid dehydrogenase (17HSD) family has been identified to be of significance in hormonedependent tissues. 17HSD1 and 17HSD2 are the main 17HSD enzymes involved in breast cancer investigated this far, but it is possible that other hormone-regulating enzymes have a similar role. 17HSD5 and 17HSD12 are associated with sex steroid metabolism, and 17HSD14 is a newly discovered enzyme that may be involved in the estrogen balance. The mRNA expression of 17HSD5, 17HSD12, and 17HSD14 were analyzed in 131 breast cancer specimens by semiquantitative real-time PCR. The results were compared with recurrencefree survival and breast cancer-specific survival of the patients. The breast cancer cell lines MCF7, SKBR3, and ZR75-1 were transiently transfected with 17HSD14 to investigate any possible effect on estradiol levels. We found that high 17HSD5 was related to significantly higher risk of late relapse in estrogen receptor (ER)-positive patients remaining recurrence-free later than 5 years after diagnosis (P = 0.02). No relation to 17HSD12 expression was found, indicating that 17HSD12 is of minor importance in breast cancer. Patients with ER-positive tumors with high expression levels of 17HSD14 showed a significantly better prognosis about recurrence-free survival (P = 0.008) as well as breast cancerspecific survival (P = 0.01), confirmed by multivariate analysis (P = 0.04). Transfection of 17HSD14 in the human breast cancer cells MCF7 and SKBR3 significantly decreased the levels of estradiol, presenting an effect of high expression

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

17β-Hydroxysteroid Dehydrogenase 14 Affects Estradiol Levels in Breast Cancer Cells and Is a Prognostic Marker in Estrogen Receptor–Positive Breast Cancer

Jansson¹,

Gunnarsson

Cohen³

et al. 2006

Cancer Research

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show abstract

“…These uncharacterised proteins could have an important physiological or pathological role and therefore serve as therapeutic targets [2]. For several serious and frequent diseases such as hormone dependent carcinomas, metabolic syndrome, diabetes mellitus, degenerative defects including Alzheimer's disease, the underlying pathogenesis involved members of the short-chain dehydrogenase/reductase (SDR) protein superfamily [3,4]. This superfamily with more than 163,000 members, found in all forms of life, represents one of the largest and oldest protein superfamilies of all [5].…”

Section: Introductionmentioning

confidence: 99%

Biochemical properties of human dehydrogenase/reductase (SDR family) member 7

Štambergová

Škarydová

Dunford

et al. 2014

Chemico-Biological Interactions

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“…Conserved residues of the SDR family, such as the active-site tetrad of N-S-Y-K [32], the structure association motif NNAG [33] and activity association motif PGXXXT [32], were highly conserved in the ChKRED15 sequence (Fig. 3).…”

Section: Identification and Sequence Analysis Of Chkred15mentioning

confidence: 99%

Bioreductive production of enantiopure (S)-duloxetine intermediates catalyzed with ketoreductase ChKRED15

Ren

Liu

Pei

et al. 2015

Journal of Molecular Catalysis B: Enzymatic

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Bioinformatic identification and characterization of new members of short-chain dehydrogenase/reductase superfamily

Cited by 20 publications

References 10 publications

17β-Hydroxysteroid Dehydrogenase 14 Affects Estradiol Levels in Breast Cancer Cells and Is a Prognostic Marker in Estrogen Receptor–Positive Breast Cancer

17β-Hydroxysteroid Dehydrogenase 14 Affects Estradiol Levels in Breast Cancer Cells and Is a Prognostic Marker in Estrogen Receptor–Positive Breast Cancer

Biochemical properties of human dehydrogenase/reductase (SDR family) member 7

Bioreductive production of enantiopure (S)-duloxetine intermediates catalyzed with ketoreductase ChKRED15

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