Bioinformatic characterization of a triacylglycerol lipase produced by
            <i>Aspergillus flavus</i>
            isolated from the decaying seed of
            <i>Cucumeropsis mannii</i>

Ezema, Benjamin E.; Omeje, Kingsley O.; Bill, Roslyn M.; Goddard, Alan D.; Eze, Sabinus Oscar Onyebuchi; Fernández-Castañé, Alfred

doi:10.1080/07391102.2022.2035821

Cited by 8 publications

(3 citation statements)

References 81 publications

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“…23,24 The lipase gene was confirmed in Pseudomonas aeruginosa 7 and Aspergillus flavus. 25 The ability of the organism to hydrolyze the substrate clearly shows that the genome harbors the relevant gene. Parameters influencing enzyme production include temperature, incubation period, pH, etc.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Culture Conditions for Lipase Production by Pseudomonas aeruginosa ECS3

2023

TJNPR

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Section: Resultsmentioning

confidence: 99%

Optimization of Culture Conditions for Lipase Production by Pseudomonas aeruginosa ECS3

2023

TJNPR

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“…Moreover, some recently isolated fungi are also documented as they can produce lipase enzymes such as Rhizopus oryzae R1 (Helal et al, 2021), Stemphylium lycopersici, Sordida sp. (Rocha et al, 2020), Aspergillus niger 13 F, Fusarium solani 7 F (Patel and Shah, 2020), Aspergillus flavus (Ezema et al, 2022), Aspergillus terreus AH-F2 (Shabbir and Mukhtar, 2018), and Thermomyces lanuginosus (Tišma et al, 2019). The production of lipases by fungi is varied according to the fungal organism and composition of the growth medium and physical conditions such as the nitrogen source, carbon source, pH, and temperature (Pandey et al, 2016).…”

Section: Fungal Sources Of Lipasesmentioning

confidence: 99%

Industrial applications of fungal lipases: a review

Kumar¹,

Verma²,

Dubey³

et al. 2023

Front. Microbiol.

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Fungal lipases (triacylglycerol acyl hydrolases EC 3.1.1.3) are significant industrial enzymes and have several applications in a number of industries and fields. Fungal lipases are found in several species of fungi and yeast. These enzymes are carboxylic acid esterases, categorized under the serine hydrolase family, and do not require any cofactor during the catalyzing of the reactions. It was also noticed that processes including the extraction and purification of lipases from fungi are comparatively easier and cheaper than other sources of lipases. In addition, fungal lipases have been classified into three chief classes, namely, GX, GGGX, and Y. Fungal lipases have applications not only in the hydrolysis of fats and oils (triglycerides) but are also involved in synthetic reactions such as esterification, acidolysis, alcoholysis, interesterification, and aminolysis. The production and activity of fungal lipases are highly affected by the carbon source, nitrogen source, temperature, pH, metal ions, surfactants, and moisture content. Therefore, fungal lipases have several industrial and biotechnological applications in many fields such as biodiesel production, ester synthesis, production of biodegradable biopolymers, formulations of cosmetics and personal care products, detergent manufacturing, degreasing of leather, pulp and paper production, textile industry, biosensor development, and drug formulations and as a diagnostic tool in the medical sector, biodegradation of esters, and bioremediation of wastewater. The immobilization of fungal lipases onto different carriers also helps in improving the catalytic activities and efficiencies of lipases by increasing thermal and ionic stability (in organic solvents, high pH, and temperature), being easy to recycle, and inducing the volume-specific loading of the enzyme onto the support, and thus, these features have proved to be appropriate for use as biocatalysts in different sectors.

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“…Production, purification, and characterization of new lipases to enhance industrial microbial lipase candidates are crucial since they have extensively been used as biocatalysts in industrial and biotechnological applications [10,27,28].…”

Section: Purification Of C Diffluens D44 Lipase(s)mentioning

confidence: 99%

Cryptococcus diffluens D44 tarafından üretilen lipaz(lar)ın saflaştırılması ve karakterizasyonuna ait ön çalışma

Büyük

Pinar

2023

International Journal of Advances in Engineering and Pure Sciences

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In the present work, preliminary purification, and characterization of lipases from Cryptococcus diffluens D44, which was isolated from petroleum sludge, were performed. In the purification steps, subsequential to acetone precipitation, lipases from C. diffluens D44 were purified by DEAE Sepharose resulting in two different peaks, named Lip1 and Lip4. Sephadex G-100 size-exclusion chromatography was also performed for further purification of Lip1 and Lip4 and resulted in three different lipases as Lip1-1 (1.0 purification fold with 2.4% recovery), Lip1-2 (0.8 purification fold with 7.2% recovery), and Lip4-1 (1.2 purification fold with 4.5% recovery). As a result of characterization studies of these three lipases resulting from different peaks, optimum temperatures were found as 60 °C, 65 °C, and 65 °C for Lip1-1, Lip1-2, and Lip4-1, respectively. Furthermore, thermal stability studies were conducted at 50 °C, 60 °C, and 70 °C, and lipases of C. diffluens D44 maintained over 70% of their initial activity at 50 °C. The optimum pH for Lip1-1 and Lip1-2 was pH 9.0 although pH 5.0 was for Lip4-1. Considering the organic solvent effect on lipase activity, 10% methanol enhanced the relative activity of Lip1-1 and Lip4-1 while 10% ethanol caused a decrease in the relative activity of lipases except for Lip1-2. According to the indicated features based on the results, these different lipases from C. diffluens D44 could be promising candidates for industrial and biotechnological applications. To the best of our knowledge, this is the first study on the purification of lipases from C. diffluens D44.

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Bioinformatic characterization of a triacylglycerol lipase produced by Aspergillus flavus isolated from the decaying seed of Cucumeropsis mannii

Cited by 8 publications

References 81 publications

Optimization of Culture Conditions for Lipase Production by Pseudomonas aeruginosa ECS3

Optimization of Culture Conditions for Lipase Production by Pseudomonas aeruginosa ECS3

Industrial applications of fungal lipases: a review

Cryptococcus diffluens D44 tarafından üretilen lipaz(lar)ın saflaştırılması ve karakterizasyonuna ait ön çalışma

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