Bioinformatic characterization and gene expression pattern of apoptosis inhibitor from Macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus

Arockiaraj, Jesu; Vanaraja, Puganeshwaran; Easwvaran, Sarasvathi; Singh, Arun; Othman, Rofina Yasmin; Bhassu, Subha

doi:10.1016/j.fsi.2011.09.008

Cited by 18 publications

(8 citation statements)

References 36 publications

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“…However, on the other hand, CcBIRC7 was widely expressed in various normal tissues, which suggested it might be processed different functions due to different species of genus. Due to lack of published BIRC7 gene expression pattern data in non-mammal species, the indirect evidence from the same IAP family while the different member as IAP-5 from invertebrate Macrobrachium rosenbergii showed that it was expressed in all the 10 tissues analyzed, including hepatopancreas, gill, brain, intestine, muscle, stomach and so on [52], which is in consistent with our studies.…”

Section: Discussionsupporting

confidence: 90%

“…Although CCRV had the weakly effect on CcBIRC7 gene expression compared with above two bacteria, it can be perceived that CcBIRC7 mRNA expression was induced by the virus. CcBIRC7 mRNA expression pattern response to virus was similar with IAP-5 from M. rosenbergii challenged to infectious hypodermal and hematopoietic necrosis virus (IHHNV), the latter was highly induced in hepatopancreas and peaked up to 7.5 times above the control after 12 h post infection [52]. Both of them confirmed what IAPs expression can be stimulated through virus infection and it plays a crucial role in innate immune system of many species [4].…”

Section: Discussionsupporting

confidence: 63%

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BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catﬁsh Hemorrhage Reovirus

Liu

Wang

et al. 2012

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 63%

BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catﬁsh Hemorrhage Reovirus

Liu

Wang

et al. 2012

Fish & Shellfish Immunology

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“…The tissues were rapidly frozen in liquid nitrogen and stored at −80°C until total RNA extraction. The 12 hour time point was chosen based on our previous work regarding immune related genes from M. rosenbergii in response to pathogen such as viruses showing significant gene expression at this time point [ 32 - 35 ].…”

Section: Methodsmentioning

confidence: 99%

RNA-seq analysis of Macrobrachium rosenbergii hepatopancreas in response to Vibrio parahaemolyticus infection

et al. 2015

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BackgroundThe Malaysian giant freshwater prawn, Macrobrachium rosenbergii, is an economically important crustacean worldwide. However, production of this prawn is facing a serious threat from Vibriosis disease caused by Vibrio species such as Vibrio parahaemolyticus. Unfortunately, the mechanisms involved in the immune response of this species to bacterial infection are not fully understood. We therefore used a high-throughput deep sequencing technology to investigate the transcriptome and comparative expression profiles of the hepatopancreas from this freshwater prawn infected with V. parahaemolyticus to gain an increased understanding of the molecular mechanisms underlying the species’ immune response to this pathogenic bacteria.ResultA total of 59,122,940 raw reads were obtained from the control group, and 58,385,094 reads from the Vibrio-infected group. Via de novo assembly by Trinity assembler, 59,050 control unigenes and 73,946 Vibrio-infected group unigenes were obtained. By clustering unigenes from both libraries, a total of 64,411 standard unigenes were produced. The standard unigenes were annotated against the NCBI non-redundant, Swiss-Prot, Kyoto Encyclopaedia of Genes and Genome pathway (KEGG) and Orthologous Groups of Proteins (COG) databases, with 19,799 (30.73%), 16,832 (26.13%), 14,706 (22.83%) and 7,856 (12.19%) hits respectively, giving a final total of 22,455 significant hits (34.86% of all unigenes). A Gene Ontology (GO) analysis search using the Blast2GO program resulted in 6,007 unigenes (9.32%) being categorized into 55 functional groups. A differential gene expression analysis produced a total of 14,569 unigenes aberrantly expressed, with 11,446 unigenes significantly up-regulated and 3,103 unigenes significantly down-regulated. The differentially expressed immune genes fall under various processes of the animal immune system.ConclusionThis study provided an insight into the antibacterial mechanism in M. rosenbergii and the role of differentially expressed immune genes in response to V. parahaemolyticus infection. Furthermore, this study has generated an abundant list of transcript from M.rosenbergii which will provide a fundamental basis for future genomics research in this field.Electronic supplementary materialThe online version of this article (doi:10.1186/s13099-015-0052-6) contains supplementary material, which is available to authorized users.

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“…Apoptosis inhibitor (HQ668090) protein contained an IAP domain which was highly expressed in hepatopancreas (Arockiaraj et al . ). NF kappa B inhibitor transcription factors (HQ668091) play an important role in inhibiting apoptosis by organizing the expression of different type of genes that regulate the prawn defence system (Arockiaraj et al .…”

Section: Immune Systemmentioning

confidence: 97%

Impacts of environmental and biological stressors on immune system ofMacrobrachium rosenbergii

Kumaresan

Palanisamy

Pasupuleti

et al. 2016

Reviews in Aquaculture

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Macrobrachium rosenbergii commonly called giant freshwater prawn is a widely farmed crustacean in freshwater. Similar to other aquatic organisms, their growth and well-being is influenced by various physical, chemical and biological factors. We discuss about the critical growth limiting factors as well as disease causing agents and the potential immune molecules of M. rosenbergii that are proved to involve in preventing and/or responding to those limiting factors.

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Bioinformatic characterization and gene expression pattern of apoptosis inhibitor from Macrobrachium rosenbergii challenged with infectious hypodermal and hematopoietic necrosis virus

Cited by 18 publications

References 36 publications

BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catﬁsh Hemorrhage Reovirus

BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catﬁsh Hemorrhage Reovirus

RNA-seq analysis of Macrobrachium rosenbergii hepatopancreas in response to Vibrio parahaemolyticus infection

Impacts of environmental and biological stressors on immune system ofMacrobrachium rosenbergii

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