Bioinformatic Analysis of Prognostic Value of Pyroptosis-Related Genes and Its Effect on Immune Cell Infiltration in Pancreatic Adenocarcinoma

Abstract

Purpose Pancreatic adenocarcinoma has a poor prognosis and chemotherapy has its limitation due to tumor heterogeneity. It is essential to find novel targets involving in tumorigenesis of pancreatic adenocarcinoma. Growing evidences indicated that pyroptosis is involved in tumorigenesis of many cancers, but the relationship between pyroptosis and pancreatic adenocarcinoma still remains to be elucidated. Our object is to explore whether pyroptosis-related different expression genes have association with survivals of pancreatic adenocarcinoma patients and the mechanism they may participate in. Besides, we also analyzed their effect on immune cell infiltration in tumor microenvironment. Patients and Methods We used the bioinformatic analysis tool including GEPIA, cBioPortal, STRING, GeneMANIA, R software 4.03 and TIMER2.0 to investigate the different expression, prognostic value, protein-protein interaction, gene ontology, pathway and effect of immune cell infiltration of pyroptosis-related genes in PAAD. Results Many pyroptosis-related genes express differently between pancreatic adenocarcinoma and normal tissues and they are associated with survival of PAAD. GABARAP and IL18 may play a key role in tumorigenesis of PAAD, for they are connected with overall survival, disease free survival and pathological stages at the same time. The function of pyroptosis-related genes includes cytokine production, endopeptidase activity, regulation of inflammation and inflammasome complex and pyroptosis-related genes have effect on immune cells infiltration in PAAD microenvironment. Conclusion Lots of pyroptosis-related DEGs may get involved in pathogenesis of PAAD and their high expression have an effect on survival. GABARAP and IL18 could be valuable research targets of PAAD.

Pyroptosis-related gene-based prognostic signature for predicting the overall survival of oral squamous cell carcinoma patients

“…Cao et al identified a series of long non-coding RNAs regulating the progression of pyroptosis and then established a pyroptosis-related lncRNA prognostic model for ovarian cancer (10). In addition, many reports confirmed that the pyroptosis level was significantly correlated to immunotherapy response in diverse types of human cancers such as glioma (11), pancreatic adenocarcinoma (12), and esophageal adenocarcinoma (13). These studies further determined the crucial value of pyroptosis in cancer, as well as the patient prognosis.…”

The Pyroptosis-Related Gene Prognostic Index Associated with Tumor Immune Infiltration for Pancreatic Cancer

“…It has been shown that pyroptosis induced by caspase-1 can suppress the proliferation, migration, invasion, and cell spheroid formation of PC cells [ 26 ]. Some pyroptosis-related genes (PRGs) may be valuable research targets for PC, and their expression can be combined with specific immune characteristics to predict the survival benefit to patients [ 27 , 28 ]. However, researchers have speculated that pyroptosis can also play a dual role in the progression and therapeutic response of PC, but studies of pyroptosis in PC are scarce [ 29 ].…”