Bioimpedance Analysis of L929 and HaCaT Cells in Low Frequency Range

Teixeira, Viviane S.; Kalckhoff, Jan-Patrick; Krautschneider, Wolfgang H.; Schroeder, Dietmar

doi:10.1515/cdbme-2018-0029

Cited by 9 publications

(8 citation statements)

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“…They found that the resistance of the cancer cells was significantly lower than that of the normal cells, while the capacitance was not significantly different. Teixeira et al 17 carried out EIS using a 4-terminal setup to distinguish mouse fibroblasts (L929) from human keratinocytes (HaCaT). On a Nyquist diagram, L929 showed only one dispersion while HaCaT showed 2 dispersions, indicated by 2 semi-circles.…”

Section: Introductionmentioning

confidence: 99%

Characterising Vascular Cell Monolayers Using Electrochemical Impedance Spectroscopy and a Novel Electroanalytical Plot

Bussooa

2020

NSA

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Introduction: Biological research relies on the culture of mammalian cells, which are prone to changes in phenotype during experiments involving several passages of cells. In regenerative medicine, specifically, there is an increasing need to expand the characterisation landscape for stem cells by identifying novel stable markers. This paper reports on a novel electric cell-substrate impedance sensing-based electroanalytical diagram which can be used for the "electrical characterisation" of cell monolayers consisting of smooth muscle cells, endothelial cells or co-culture. Materials and Methods: Interdigitated electrodes were microfabricated using standard cleanroom procedures and integrated into cell chambers. Electrochemical impedance spectroscopy data were acquired for 2 vascular cell types after they formed monolayers on the electrodes. Results and Discussion: A Mean impedance per unit area vs Mean phase plots provided a reproducible, visually obvious and statistically significant method of characterising cell monolayers. This electroanalytic diagram has never been used in previous papers, but it confirms findings by other research groups using similar approaches that the complex impedance spectra of different cell type are different. Further work is required to determine whether this method could be extended to other cell types, and if this is the case, a library of "signature spectra" could be generated for "electrical characterisation" of cells.

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Section: Introductionmentioning

confidence: 99%

Characterising Vascular Cell Monolayers Using Electrochemical Impedance Spectroscopy and a Novel Electroanalytical Plot

Bussooa

2020

NSA

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“…The diameter of HaCaT cells varies between 20 and 25 µm. [ 41 ] Considering the size of the HaCaT cell, the pore size was sufficient for the cell to settle. Moreover, SEM results displayed that the synthesized cryogels had a dense, thin, and interconnected pore morphology and a macroporous structure.…”

Section: Resultsmentioning

confidence: 99%

Phyllanthus emblica‐Loaded Cryogels for Improved Wound Care: Characterization and In Vitro Studies

Canatar,

Özdaş,

Baydemir Peşint

2024

Macro Materials & Eng

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Wound dressings developed by combining plant extracts with polymers have made a great progress in wound care treatment. One plant with remarkable healing properties is Phyllanthus emblica Linn (P. emblica), which is described as having potent antioxidant, antimicrobial and anti‐inflammatory properties. The aim of this study is to evaluate the biocompatibility of P. emblica‐loaded polyvinyl alcohol/gelatin‐based cryogels (PVA/Gel/P.emblica) through cytotoxicity and proliferation tests in HaCaT cells and examine their potential in wound dressing applications. Accordingly, PVA/Gel/P.emblica cryogels are successfully synthesized and characterization studies and in vitro cell culture studies are performed. The swelling tests and Brunauer–Emmett–Teller analysis results show that swelling and surface area properties of cryogels increase with increasing P. emblica amounts. Morphological results display that the cryogels have a dense, interconnected pore morphology and a macroporous structure. 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, trypan blue exclusion, and live–dead assay results reveal that P. emblica enhances cell proliferation, increases cell number, and improves cell viability. Based on the scanning electron microscope, immunofluorescence, and Giemsa staining images, it is observed that P. emblica promotes cell attachment, proliferation, and penetration. These findings confirm that PVA/Gel/P.emblica cryogels are suitable for use as wound dressing materials and can be developed with further studies.

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“…The PVA/Gel and P. emblica loaded PVA/Gel cryogels possess similar structures and pore sizes of 30-120 µm and 30-100 µm, respectively. The diameter of HaCaT cells varies between 20-25 micrometer [50]. Considering the size of the Hacat cell, the pore size was sufficient for the cell to settle.…”

Section: Morphology Analysismentioning

confidence: 99%

Phyllanthus emblica Loaded Cryogels for Improved Wound Care: Characterizationand In Vitro Studies

CANATAR,

ÖZDAŞ,

Baydemir Pesint

2024

Preprint

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Phyllanthus emblica (P. emblica) has been described in many studies to have multiple therapeutic activities and is considered an important part of Ayurvedic and Unani medicinal systems. This study successfully synthesized P. emblica loaded polyvinylalcohol/gelatin based cryogels (PVA/Gel/P.emblica) to examine their potential in wound dressing applications. Swelling tests, Brunauer-Emmett-Teller (BET) and morphology analysis were conducted within the scope of characterization studies. In vitro cell culture studies were performed using HaCaT cells to illustrate the suitability of the synthesized cryogels as wound dressing material. The swelling tests and BET analysis results showed that swelling and surface area properties of cryogels increased with increasing P. emblica amounts. Morphological results displayed that the cryogels had a dense, interconnected pore morphology and a macroporous structure. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trypan blue exclusion and live-dead assay results revealed that adding of P. emblica into cryogels led to enhanced cell proliferation, increased cell number, and improved cell viability. Based on the phase contrast microscope, scanning electron microscope (SEM), immunofluorescence and giemsa staining images, it was observed that P. emblica promoted cell attachment, proliferation and penetration. In conclusion, it has been shown that PVA/Gel/P.emblica cryogels are suitable for use as wound dressing materials and can be developed with further studies.

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Bioimpedance Analysis of L929 and HaCaT Cells in Low Frequency Range

Cited by 9 publications

References 0 publications

<p>Characterising Vascular Cell Monolayers Using Electrochemical Impedance Spectroscopy and a Novel Electroanalytical Plot</p>

<p>Characterising Vascular Cell Monolayers Using Electrochemical Impedance Spectroscopy and a Novel Electroanalytical Plot</p>

Phyllanthus emblica‐Loaded Cryogels for Improved Wound Care: Characterization and In Vitro Studies

<strong><em></em></strong><em>Phyllanthus emblica</em> Loaded Cryogels for Improved Wound Care: Characterizationand In Vitro Studies

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