Bioimaging TOF‐SIMS: High resolution 3D imaging of single cells

Nygren, Håkan; Hagenhoff, Birgit; Malmberg, Per; Nilsson, Mikael; Richter, Katrin

doi:10.1002/jemt.20502

Cited by 97 publications

(89 citation statements)

References 21 publications

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“…[19][20][21][22] Typically, Bi cluster ToF-SIMS equipped with a liquid metal ion gun (LMIG) has been used for image analysis because the ionized Bi n q + clusters (n = 1-7, q = 1, 2) achieve high brightness. 23,24) Also, it was reported that Bi monomer ions were the optimum primary ion species for analyzing hydrogen and deuterium in materials. 25,26) Concerning steels samples, dynamic SIMS has been shown to be an effective method for detecting deuterium or hydrogen in different steels.…”

Section: Micro-portion Image Analysis Of Light Elements In Fe-cr Basementioning

confidence: 99%

Micro-Portion Image Analysis of Light Elements in Fe–Cr Based Alloys by Time-of-Flight Secondary Ion Mass Spectrometry

et al. 2017

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Section: Micro-portion Image Analysis Of Light Elements In Fe-cr Basementioning

confidence: 99%

Micro-Portion Image Analysis of Light Elements in Fe–Cr Based Alloys by Time-of-Flight Secondary Ion Mass Spectrometry

et al. 2017

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“…Indeed, this study, along with the previous one, shows that the chemical composition of animal cells can be reconstructed in three dimensions by ToF-SIMS imaging in combination with repeated sputter cycles that erode the sample in a layer-by-layer fashion and thereby make the inner structure of the cells accessible for SIMS analysis. A similar study using C 60 for sputtering and Bi þ 3 for analysis has been reported on freeze-fractured, freeze-dried thyroid tumor cells in which it is claimed that internal cell compartments were imaged (Nygren et al, 2007). However, in this case the authors could only detect and image, sodium, potassium, m/z 86, which is not identified, but could be a fragment of leucine/isoleucine or choline, and m/z 184 from phosphocholine.…”

Section: Normal Rat Kidney Cellsmentioning

confidence: 69%

Developments in molecular SIMS depth profiling and 3D imaging of biological systems using polyatomic primary ions

Fletcher¹,

Lockyer²,

Vickerman³

2010

Mass Spectrometry Reviews

142

119

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In principle mass spectral imaging has enormous potential for discovery applications in biology. The chemical specificity of mass spectrometry combined with spatial analysis capabilities of liquid metal cluster beams and the high yields of polyatomic ion beams should present unprecedented ability to spatially locate molecular chemistry in the 100 nm range. However, although metal cluster ion beams have greatly increased yields in the m/z range up to 1000, they still have to be operated under the static limit and even in most favorable cases maximum yields for molecular species from 1 µm pixels are frequently below 20 counts. However, some very impressive molecular imaging analysis has been accomplished under these conditions. Nevertheless although molecular ions of lipids have been detected and correlation with biology is obtained, signal levels are such that lateral resolution must be sacrificed to provide a sufficient signal to image. To obtain useful spatial resolution detection below 1 µm is almost impossible. Too few ions are generated! The review shows that the application of polyatomic primary ions with their low damage cross-sections offers hope of a new approach to molecular SIMS imaging by accessing voxels rather than pixels to thereby increase the dynamic signal range in 2D imaging and to extend the analysis to depth profiling and 3D imaging. Recent data on cells and tissue analysis suggest that there is, in consequence, the prospect that a wider chemistry might be accessible within a sub-micron area and as a function of depth. However, these advances are compromised by the pulsed nature of current ToF-SIMS instruments. The duty cycle is very low and results in excessive analysis times, and maximum mass resolution is incompatible with maximum spatial resolution. New instrumental directions are described that enable a dc primary beam to be used that promises to be able to take full advantage of all the capabilities of the polyatomic ion beam. Some new data are presented that suggest that the aspirations for these new instruments will be realized. However, although prospects are good, the review highlights the continuing challenges presented by the low ionization efficiency and the complications that arise from matrix effects.

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“…Mass spectrometric imaging is capable of detecting lipid species in intact biological cells and tissue, and providing lateral resolution down to the micron scale (13)(14)(15) ) and recording the ejected secondary ions as a function of their m/z and point of origin on the sample to form a "chemical map". The introduction of polyatomic primary ion beams such as C 60 + has facilitated the analysis of molecular species beyond the sample surface allowing complex chemical distributions to be studied in both two and three dimensions ( 14,17 ).…”

Section: Sampling the X Laevis Embryosmentioning

confidence: 99%

Spatiotemporal lipid profiling during early embryo development of Xenopus laevis using dynamic ToF-SIMS imaging

Tian

Fletcher

Thuret

et al. 2014

Journal of Lipid Research

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This article is available online at http://www.jlr.org GC-MS/LC-MS. Comprehensive work on the membrane composition of the X. laevis oocyte has been reported by Hill et al. ( 3 ). Lipid species in the mass range m/z 400-950 were detected. The plasma membrane is rich in glycerophosphocholine (GPCho), SM, and glycerophosphoinositol (GPIns), as well as cholesterol, comprising approximately 20% of the total lipid extraction. Huang, Liang, and Kam ( 4 ) examined the fatty acid composition of egg yolk in three anuran species. Koek et al. ( 5 ) analyzed approximately 10% of the cytoplasm in a single X. laevis oocyte using GC/MS. The detected compounds include organic acids, fatty acids (saturated, unsaturated, and hydroxy fatty acids ranging from C8 to C28), amino acids, alcohols, and sugars. These techniques, based on solvent extraction, do not provide any information on spatial localization beyond the fraction which was extracted; however, they offer qualitative and quantitative information about the composition of the cytoplasm with respect to key lipid compounds ( 6 ).A current challenge in lipid biology studies is determining the spatial location of multiple lipids while retaining compositional information. Techniques such as scanning electron microscopy and confocal microscopy have been applied to image the frog egg and embryo to investigate the mechanisms of biological processes and embryo development. Monroy and Baccetti ( 7 ) fi rst revealed the outer surface of the plasma membrane in X. laevis eggs by means of scanning electron microscopy. The architecture of the membrane exhibits dramatic changes before and after The location and identifi cation of lipids is essential to understand their role in biological processes. Their distribution in a cell membrane, nucleus, or other organelles is responsible for their function in inter-and intra-cellular communication and signaling ( 1, 2 ). To date, lipid profi ling of Xenopus laevis embryos has been accomplished using a selective organic extraction methodology followed by

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Bioimaging TOF‐SIMS: High resolution 3D imaging of single cells

Cited by 97 publications

References 21 publications

Micro-Portion Image Analysis of Light Elements in Fe–Cr Based Alloys by Time-of-Flight Secondary Ion Mass Spectrometry

Micro-Portion Image Analysis of Light Elements in Fe–Cr Based Alloys by Time-of-Flight Secondary Ion Mass Spectrometry

Developments in molecular SIMS depth profiling and 3D imaging of biological systems using polyatomic primary ions

Spatiotemporal lipid profiling during early embryo development of Xenopus laevis using dynamic ToF-SIMS imaging

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