Bioimaging of Pb and STIM1 in mice liver, kidney and brain using Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) and immunohistochemistry

Togao, Masao; Nakayama, Shouta M.M.; Ikenaka, Yoshinori; Mizukawa, Hazuki; Makino, Yoshihide; Kubota, Ayano; Matsukawa, Tetsuya; Yokoyama, Kazuhito; Hirata, Takafumi; Ishizuka, Mayumi

doi:10.1016/j.chemosphere.2019.124581

Cited by 34 publications

(15 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(2020) reported a similar inhomogeneous distribution of Pb in the kidney; however, the area with the highest Pb concentration was located more deeply in the kidney parenchyma (at the cortico-medullary junction). The levels of Pb in kidneys in that study [43] were, however, much higher both in so-called low (about eightfold) and high (fortyfold) conditions compared with our experiment, which could affect the Pb distribution in kidney tissue.…”

Section: Deregulation Of the Storage Of Basic Elements (Na K Ca Andcontrasting

confidence: 82%

A Clearance Period After Soluble Lead Nanoparticle Inhalation did not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response

Dumková

Smutná

Vrlíková

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The inhalation of metal (including lead) nanoparticles poses a real health issue to people and animals living in polluted and/or industrial areas. In this study, we exposed mice to lead(II) nitrate nanoparticles [Pb(NO3)2 NPs], which represent a highly soluble form of lead, by inhalation. We aimed to uncover possible differences in the effects of lead exposure on individual target organs and to reveal potential variability in the clearance of lead from organs after inhalation of soluble and insoluble lead particles. Mice were exposed to Pb(NO3)2 NPs in whole-body inhalation chambers for up to 11 weeks. We examined (i) lead biodistribution in target organs using laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) and atomic absorption spectrometry (AAS), (ii) lead effect on histopathological changes and immune cells response in secondary target organs and (iii) the clearance ability of target organs (lung, liver, kidney, spleen, bone and blood). Results: After exposure to Pb(NO3)2 NPs, the lead concentration was the highest in femur at all designated time points with the exception of 3-day inhalation. In contrast to other organs, the ability to clear the lead from the femur was very low. In the lungs, Pb(NO3)2 NP inhalation induced serious structural changes; lung damage was present even after a 5-week clearance period despite the lead being almost completely eliminated from the lung tissue. Furthermore, in secondary target organs (kidney and liver), Pb(NO3)2 NPs induced several morphological defects, with alterations that remained visible after the clearance period even though the tissue lead concentrations decreased to minimal values. The numbers of lung and liver macrophages were significantly decreased after 11-week Pb(NO3)2 NP inhalation; conversely, abundance of alpha-smooth muscle actin (α-SMA)-positive cells, which are responsible for augmented collagen production, was increased in both tissues. Moreover, the expression of nuclear factor κB (NF-κB) and selected cytokines (tumour necrosis factor alpha [TNF-α], transforming growth factor beta 1 [TGFβ1], interleukin 6 [IL-6], IL-1α and IL-1β) displayed a tissue-specific response to lead exposure. Conclusions: Taken together, diminished inflammatory response in lung and liver after Pb(NO3)2 NPs inhalation was associated with prolonged negative effect of lead on tissues, as demonstrated by enhanced pathological changes in target organs, even after a 5-week clearance period that contrasts to the stronger effect observed previously after the clearance of insoluble lead nanoparticles.

show abstract

Section: Deregulation Of the Storage Of Basic Elements (Na K Ca Andcontrasting

confidence: 82%

A Clearance Period After Soluble Lead Nanoparticle Inhalation did not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response

Dumková

Smutná

Vrlíková

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The control group received only distilled water for the same period. The choice of Pb levels of exposure for the current study was based on the previous study in mice that had a dose-dependent Pb tissue accumulation at 100 mg/L and 1000 mg/L lead acetate concentrations [23]. At the end of the exposure period, rats were euthanized under carbon dioxide with sevoflurane.…”

Section: Laboratory Animals and Exposurementioning

confidence: 99%

An Investigation of the Wild Rat Crown Incisor as an Indicator of Lead (Pb) Exposure Using Inductively Couple Plasma Mass Spectrometry (ICP-MS) and Laser Ablation ICP-MS

Kataba

Nakayama

Nakata

et al. 2021

IJERPH

Self Cite

View full text Add to dashboard Cite

Lead (Pb) is a metal toxicant of great public health concern. The present study investigated the applicability of the rat incisor in Pb exposure screening. The levels of lead in teeth (Pb-T) in the crown and root of incisors in laboratory Pb-exposed Sprague Dawley rats were quantified using inductively coupled plasma mass spectrometry (ICP-MS). The crown accumulated much Pb-T than the root of the Sprague Dawley rat incisor. The levels of lead in blood (Pb-B) were positively correlated with the Pb-T in the crown and root incisors of the Sprague Dawley rats. As an application of the Pb-T crown results in experimental rats, we subsequently analyzed the Pb-T in the crown incisors of Pb-exposed wild rats (Rattus rattus) sampled from residential sites within varying distances from an abandoned lead–zinc mine. The Pb-T accumulation in the crown of incisors of R. rattus rats decreased with increased distance away from the Pb–Zn mine. Furthermore, the Pb-T was strongly correlated (r = 0.85) with the Pb levels in the blood. Laser ablation ICP-MS Pb-T mappings revealed a homogenous distribution of Pb in the incisor with an increased intensity of Pb-T localized in the tip of the incisor crown bearing an enamel surface in both Sprague Dawley and R. rattus rats. These findings suggest that Pb-T in the crown incisor may be reflective of the rat’s environmental habitat, thus a possible indicator of Pb exposure.

show abstract

“…Upon oral entry, Pb typically accumulates in three compartments: bone, blood, and soft organs. In soft organs, orally administered Pb distributes in the following order kidney→spleen→liver [41,42]. Deposition of Pb in skeletal elements upon oral as well as inhalation entry is well documented [2], so that here we focused our attention to soft organs.…”

Section: Discussionmentioning

confidence: 99%

Macrophage-Mediated Tissue Response Evoked By Subchronic Inhalation of Lead Oxide Nanoparticles Is Associated With The Alteration of Phospholipases C And Cholesterol Transporters

Smutná

Dumková

Kristeková

et al. 2021

Preprint

View full text Add to dashboard Cite

BackgroundInhalation of lead oxide nanoparticles (PbO NPs), which are emitted to the environment by high-temperature technological processes, heavily impairs target organs. These nanoparticles pass through the lung barrier and are distributed via the blood into secondary target organs, where they cause numerous pathological alterations. Here, we studied in detail, macrophages as specialized cells involved in the innate and adaptive immune response in selected target organs to unravel their potential involvement in reaction to subchronic PbO NP inhalation. In this context, we also tackled possible alterations in lipid uptake in the lungs and liver, which is usually associated with foamy macrophage formation. MethodsFemale mice CD-1 (ICR), as a model organism, were placed into whole-body inhalation chambers and continuously exposed to PbO NPs at a concentration of 0.956 × 106 NPs/cm3 for 11 weeks (24/7). At the end of the exposure period, target organs (lung, liver, kidney, and spleen) were collected for analyses.ResultsThe histopathological analysis of PbO NP exposed lung revealed serious chronic inflammation of lung tissues. The number of foamy macrophages was significantly increased in lung, and they contained numerous cholesterol crystals. PbO NP inhalation induced changes in expression of phospholipases C (PLC) as enzymes linked to macrophage-mediated inflammation in lungs. In the liver, the subchronic inhalation of PbO NPs caused predominantly hyperemia, microsteatosis or remodeling of the liver parenchyma, and the number of liver macrophages was also significantly increased. The expression of a scavenger receptor CD36 and transporter ABCA1, which are associated with lipid metabolism, was significantly altered in the liver. No microscopic or serious functional kidney alterations were detected after subchronic PbO NP exposure and CD68 positive cells were present in the physiological mode in the interstitial tissues. ConclusionOur study determined the association of increased cholesterol and lipid storage in targeted tissues with the alteration of scavenger receptors and phospholipases C after subchronic inhalation of PbO NPs and uncovered processes, which contribute to steatosis in liver and foamy macrophages in lungs.

show abstract

Bioimaging of Pb and STIM1 in mice liver, kidney and brain using Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) and immunohistochemistry

Cited by 34 publications

References 49 publications

A Clearance Period After Soluble Lead Nanoparticle Inhalation did not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response

A Clearance Period After Soluble Lead Nanoparticle Inhalation did not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response

An Investigation of the Wild Rat Crown Incisor as an Indicator of Lead (Pb) Exposure Using Inductively Couple Plasma Mass Spectrometry (ICP-MS) and Laser Ablation ICP-MS

Macrophage-Mediated Tissue Response Evoked By Subchronic Inhalation of Lead Oxide Nanoparticles Is Associated With The Alteration of Phospholipases C And Cholesterol Transporters

Contact Info

Product

Resources

About