Bioimaging of Metals and Biomolecules in Mouse Heart by Laser Ablation Inductively Coupled Plasma Mass Spectrometry and Secondary Ion Mass Spectrometry

Becker, J. Sabine; Breuer, U.; Hsieh, H. F.; Osterholt, Tobias; Kumtabtim, Usarat; Wu, Bei; Matusch, Andreas; Caruso, Joseph A.; Qin, Zhe

doi:10.1021/ac102256q

Cited by 74 publications

(58 citation statements)

References 43 publications

(65 reference statements)

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“…Although the kidneys appear to be Mn limited, the similar bacterial burdens in the kidneys of wild-type and CP-deficient mice suggest that CP does not contribute substantially to Mn sequestration in this organ (14). To address this possibility, IMS was employed to image metal and protein levels in infected organs (14,(61)(62)(63)(64)(65)(66). Imaging LA-ICP-MS was used to examine metal distribution in the kidneys of wild-type C57BL/6 and CP-deficient mice infected with S. aureus.…”

Section: Fig 4 Mntabc and Mnth Are Necessary For Invasive S Aureus Dmentioning

confidence: 99%

MntABC and MntH Contribute to Systemic Staphylococcus aureus Infection by Competing with Calprotectin for Nutrient Manganese

et al. 2013

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During infection, vertebrates limit access to manganese and zinc, starving invading pathogens, such as Staphylococcus aureus, of these essential metals in a process termed "nutritional immunity." The manganese and zinc binding protein calprotectin is a key component of the nutrient-withholding response, and mice lacking this protein do not sequester manganese from S. aureus liver abscesses. One potential mechanism utilized by S. aureus to minimize host-imposed manganese and zinc starvation is the expression of the metal transporters MntABC and MntH. We performed transcriptional analyses of both mntA and mntH, which revealed increased expression of both systems in response to calprotectin treatment. MntABC and MntH compete with calprotectin for manganese, which enables S. aureus growth and retention of manganese-dependent superoxide dismutase activity. Loss of MntABC and MntH results in reduced staphylococcal burdens in the livers of wild-type but not calprotectin-deficient mice, suggesting that these systems promote manganese acquisition during infection. During the course of these studies, we observed that metal content and the importance of calprotectin varies between murine organs, and infection leads to profound changes in the anatomical distribution of manganese and zinc. In total, these studies provide insight into the mechanisms utilized by bacteria to evade host-imposed nutrient metal starvation and the critical importance of restricting manganese availability during infection. Staphylococcus aureus is a commensal organism that asymptomatically colonizes nearly one-third of the population (1). However, once S. aureus breaches the epithelial barrier, the bacterium is capable of infecting nearly every organ despite the robust defenses elaborated by the host (2). This adaptability contributes to the significant morbidity and mortality associated with S. aureus infections. The emergence of methicillin-and vancomycin-resistant isolates has compounded the threat of this organism, highlighting the need to identify new therapeutics (3-7). This is of particular importance, as antibiotic resistance is prevalent in both hospital-and community-acquired isolates (4,6,8).Metals are essential for all forms of life due to their critical contributions to protein structure and enzymatic function (9-12). To combat invading pathogens, vertebrates leverage the essentiality of transition metals by restricting their availability, a process termed "nutritional immunity" (10, 13). While the most prominent example of nutritional immunity is the restriction of iron (Fe) by the host, it has recently been discovered that vertebrates also limit manganese (Mn) and zinc (Zn) availability during infection (10,(13)(14)(15)(16)(17). In fact, examination of abscesses formed during S. aureus infection has revealed that these lesions are Mn and Zn depleted (14). It was subsequently determined that the Mnand Zn-binding S100 protein calprotectin (CP) is a critical component of this nutrient-withholding response (10,14,18,19). CPdeficient mice...

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Section: Fig 4 Mntabc and Mnth Are Necessary For Invasive S Aureus Dmentioning

confidence: 99%

MntABC and MntH Contribute to Systemic Staphylococcus aureus Infection by Competing with Calprotectin for Nutrient Manganese

et al. 2013

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“…LA-ICP MS is an attractive approach because it offers the possibility of not only mapping but also absolutely quantifying these elements over a wide dynamic range [36]. Recent important applications include the monitoring of elementals in various brain disorders including stroke, Parkinson's and Alzheimer's disease, and cancer [37,38].…”

Section: Principle Methods and Instrumentationmentioning

confidence: 99%

“…Each of these techniques has its strength and weaknesses that in some case can be nicely compensated by a sister technology. Several examples exist in the literature where at least two imaging MS technologies have been employed to investigate the molecular content of tissue sections [38,[43][44][45].…”

Section: Principle Methods and Instrumentationmentioning

confidence: 99%

Imaging mass spectrometry of thin tissue sections: A decade of collective efforts

Chaurand

2012

Journal of Proteomics

100

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“…As a useful attempt, we recently applied LA-ICP-MS technique to determine and quantify the metal distribution in mouse heart. 42 In the future, it will be important to apply these powerful techniques to further investigate the metal distribution in various human diseases.…”

Section: Suggestions and Future Directionsmentioning

confidence: 99%

Trace metal imaging with high spatial resolution: Applications in biomedicine

et al. 2011

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New generations of analytical techniques for imaging of metals are pushing hitherto boundaries of spatial resolution and quantitative analysis in biology. Because of this, the application of these imaging techniques described herein to the study of the organization and dynamics of metal cations and metal-containing biomolecules in biological cell and tissue is becoming an important issue in biomedical research. In the current review, three common metal imaging techniques in biomedical research are introduced, including synchrotron X-ray fluorescence (SXRF) microscopy, secondary ion mass spectrometry (SIMS), and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). These are exemplified by a demonstration of the dopamine-Fe complexes, by assessment of boron distribution in a boron neutron capture therapy cell model, by mapping Cu and Zn in human brain cancer and a rat brain tumor model, and by the analysis Metallomics, 2011, 3, 28-37 29 of metal topography within neuromelanin. These studies have provided solid evidence that demonstrates that the sensitivity, spatial resolution, specificity, and quantification ability of metal imaging techniques is suitable and highly desirable for biomedical research. Moreover, these novel studies on the nanometre scale (e.g., of individual single cells or cell organelles) will lead to a better understanding of metal processes in cells and tissues.

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Bioimaging of Metals and Biomolecules in Mouse Heart by Laser Ablation Inductively Coupled Plasma Mass Spectrometry and Secondary Ion Mass Spectrometry

Cited by 74 publications

References 43 publications

MntABC and MntH Contribute to Systemic Staphylococcus aureus Infection by Competing with Calprotectin for Nutrient Manganese

MntABC and MntH Contribute to Systemic Staphylococcus aureus Infection by Competing with Calprotectin for Nutrient Manganese

Imaging mass spectrometry of thin tissue sections: A decade of collective efforts

Trace metal imaging with high spatial resolution: Applications in biomedicine

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