Biohydrogen Production from Palm Oil Mill Effluent Pretreated by Chemical Methods Using Thermoanaerobacterium-Rich Sludge

Abstract

Abstract:The effect of alkaline and acid pretreatment on solubilization of solid organic matter in palm oil mill effluent (POME) and hydrogen production by Thermoanaerobacterium-rich sludge was studied. Organic matter solubilization of alkaline and acid pretreatments were increased up to 28 and 15.7% of initial soluble COD in POME, respectively. That corresponds to carbohydrate solubilization of 41 and 32% of initial soluble carbohydrate in POME, respectively. A maximum hydrogen production yield of 4.6 l H /l-POME was achieved 2 from POME pretreated with 1.5% w/v NaOH, which was 3-fold greater than raw POME and 1-fold greater than POME pretreated with 1.5% w/v HCl. Optimum conditions for biohydrogen production from alkaline pretreated POME using Thermoanaerobacterium-rich sludge was found at initial pH 5.5 and temperature of 60°C, which gives a maximum hydrogen production yield of 5.2 l H /l-POME and increased 51% as compared to raw POME. 2The biogas was mainly composed of hydrogen and carbon dioxide with the percentage of hydrogen ranging from 55-60% of biogas and free of methane. During the conversion of alkaline pretreated POME into hydrogen, the acetic and butyric acids were main by-products in the metabolism. The results showed that alkaline pretreatment is an effective pretreatment methods for enhancing the hydrogen production yield from POME. The use of Thermoanaerobacterium-rich sludge is promising for future engineering practice of biohydrogen production from alkaline pretreated POME.

Applicability of various pretreatment techniques to enhance the anaerobic digestion of Palm oil Mill effluent (POME): A review

“…A total biohydrogen production of 4.6 l H 2 /L-POME was attained from pre-treated POME with 1.5% w/v NaOH, which was 3 times superior to untreated POME and 1-fold superior to POME pre-treated with 1.5% w/v HCl. They also found that the optimal conditions for improved biohydrogen production from POME pretreated with alkali using Thermoanaerobacterium-rich sludge was at an initial pH of 5.5 and a temperature of 60°C, which produced the highest biohydrogen yield of 5.2 l H 2 /L-POME, which resulted in an increase of 51% in comparison to the control untreated raw POME [89].…”

“…In another study, POME enriched with Thermoanaerobacterium was pretreated with a HCl concentration range of 0-2.5% w/v by stirring for 30 min, followed by adjusting the pH, which is the investigating parameter, with 1 M NaOH. It was found that the organic matter solubilization by acid pretreatment increased up to 15.7% of the preliminary sCOD content of POME, while a carbohydrate solubilization of 32% of the initial soluble carbohydrate in POME was achieved [89].…”

Advances and Perspectives in Biohydrogen Production from Palm Oil Mill Effluent

“…Seengenyoung [54] compared acid and alkaline pretreatments carried out with different concentrations (0-2.5 w/v) of NaOH and HCl, verifying their effects on the cumulative production of H 2 . The highest cumulative hydrogen production occurred from POME pretreated with 1.5% (w/v) NaOH, reaching 4.6 L H 2 /L-POME, a value three times greater than the amount of H 2 obtained from raw POME, while the amount of H 2 obtained from pretreatment with 1.5% (w/v) HCl was 2.56 L H 2 /L-POME.…”

Application of Partial-Mixed Semi-Continuous Anaerobic Reactor for Treating Palm Oil Mill Effluent (POME) Under Mesophilic Condition

“…operated in continuous mixing mode [2][3][4]. The contents 4 109 Seengenyoung et al [8] have investigated the effect of alkaline and acid pretreatment on solubilization of solid organic matter in palm oil mill effluent (POME) and hydrogen production using enriched sludge; they have successfully converted the volatile organic compound of POME to hydrogen. Chelliapan et al [9] have converted paper mill effluent to methane in anaerobic digester at OLR of 1.56 kg COD/m .d.…”