Biohydrogels with magnetic nanoparticles as crosslinker: Characteristics and potential use for controlled antitumor drug-delivery

Barbucci, Rolando; Giani, Gabriele; Fedi, Serena; Bottari, Severino; Casolaro, Mario

doi:10.1016/j.actbio.2012.09.006

Cited by 67 publications

(45 citation statements)

References 35 publications

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“…The main objective of our research was to reformulate and develop a new nanoparticle formulation for diclofenac sodium that could replace traditional capsule and tablet oral administration and minimize or delay the onset of adverse side effects commonly associated with NSAIDs. Nanoparticles have been used for the development of a variety of different drug delivery systems [1], [14], [16]–[20]. Recent studies have shown the use of polymer based nanoparticles in the reformulation of diclofenac for ophthalmic and colonic us with promising results [13], [21]–[23].…”

Section: Discussionmentioning

confidence: 99%

Design and Optimization of PLGA-Based Diclofenac Loaded Nanoparticles

Cooper

Harirforoosh

2014

PLoS ONE

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Drug based nanoparticle (NP) formulations have gained considerable attention over the past decade for their use in various drug formulations. NPs have been shown to increase bioavailability, decrease side effects of highly toxic drugs, and prolong drug release. Nonsteroidal anti-inflammatory drugs such as diclofenac block cyclooxygenase expression and reduce prostaglandin synthesis, which can lead to several side effects such as gastrointestinal bleeding and renal insufficiency. The aim of this study was to formulate and characterize diclofenac entrapped poly(lactide-co-glycolide) (PLGA) based nanoparticles. Nanoparticles were formulated using an emulsion-diffusion-evaporation technique with varying concentrations of poly vinyl alcohol (PVA) (0.1, 0.25, 0.5, or 1%) or didodecyldimethylammonium bromide (DMAB) (0.1, 0.25, 0.5, 0.75, or 1%) stabilizers centrifuged at 8,800 rpm or 12,000 rpm. The resultant nanoparticles were evaluated based on particle size, zeta potential, and entrapment efficacy. DMAB formulated NPs showed the lowest particle size (108±2.1 nm) and highest zeta potential (−27.71±0.6 mV) at 0.1 and 0.25% respectively, after centrifugation at 12,000 rpm. Results of the PVA based NP formulation showed the smallest particle size (92.4±7.6 nm) and highest zeta potential (−11.14±0.5 mV) at 0.25% and 1% w/v, respectively, after centrifugation at 12,000 rpm. Drug entrapment reached 77.3±3.5% and 80.2±1.2% efficiency with DMAB and PVA formulations, respectively. The results of our study indicate the use of DMAB for increased nanoparticle stability during formulation. Our study supports the effective utilization of PLGA based nanoparticle formulation for diclofenac.

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Section: Discussionmentioning

confidence: 99%

Design and Optimization of PLGA-Based Diclofenac Loaded Nanoparticles

Cooper

Harirforoosh

2014

PLoS ONE

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“…The water uptake (WU), or swelling degree, was determined gravimetrically following the previously reported procedure [10]. Freeze dried samples (freeze dried hydrogels containing about 10% water) were immersed in 0.15 M NaCl at room temperature and stored for more than 24 h until they reached a swelling equilibrium.…”

Section: Potentiometric Swelling and Rheological Measurementsmentioning

confidence: 99%

Influence of Alternating and Static Magnetic Fields on Drug Release from Hybrid Hydrogels Containing Magnetic Nanoparticles

Uva¹,

Pasqui²,

Mencuccini³

et al. 2014

JBNB

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Hybrid hydrogels of carboxymethylcellulose (CMC), containing two different amounts of CoFe2O4 magnetic nanoparticles (50% and 70% in relation to the quantity of the polymer) as crosslinkers, were prepared. The hybrid hydrogels were chemically and morphologically characterized and their viscoelastic properties and swelling degrees were analyzed. The hydrogels were tested as controlled drug delivery systems by applying one static and two different alternating magnetic fields. The application of the two alternating magnetic fields (AMF) to the hybrid hydrogels induced a higher release of methylene blue (MB), used as a model drug, than without the application of any magnetic field, especially at low frequency (4 Hz) and high magnetic intensity (0.5 T). In contrast, when the hybrid hydrogels were exposed to a static magnetic field (SMF) the release of MB was slowed down. Furthermore the two different amounts of magnetic nanoparticles induce different responses to the magnetic field. The greater number of nanoparticles in the CMC-NP-70 hydrogel leads to the formation of some NPs clusters limiting the drug release; conversely, the CMC-NP-50 hydrogel, containing a lower amount of nanoparticles, shows a higher release of MB vs. time. In conclusion, we were able to get a potential system for modulation of the drug delivery: the release behaviour of hybrid hydrogels can be modulated by applying alternating and static magnetic fields cyclically. A possible explanation for the release mechanism is about the structural modification of the polymeric chains that occurs when the hybrid hydrogels are exposed to the magnetic fields.* Corresponding author. M. Uva et al.117

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“…Previous research led to synthesis of a magnetic hybrid polysaccharide hydrogel that could release a drug when alternate magnetic fields (AMF) were applied [9, 10, 11]. The organic part is a carboxymethylcellulose (CMC) polymer and the inorganic part consists of cobalt-ferrite (CoFe 2 O 4 ) NPs coated with (3-aminopropyl)trimethoxysilane (APTMS) to obtain CoFe 2 O 4 -NH 2 NPs.…”

Section: Introductionmentioning

confidence: 99%

Monitoring Endothelial and Tissue Responses to Cobalt Ferrite Nanoparticles and Hybrid Hydrogels

et al. 2016

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Iron oxide nanoparticles (NPs) have been proposed for many biomedical applications as in vivo imaging and drug delivery in cancer treatment, but their toxicity is an ongoing concern. When NPs are intravenously administered, the endothelium represents the first barrier to tissue diffusion/penetration. However, there is little information about the biological effects of NPs on endothelial cells. In this work we showed that cobalt-ferrite (CoFe2O4) NPs affect endothelial cell integrity by increasing permeability, oxidative stress, inflammatory profile and by inducing cytoskeletal modifications. To overcome these problems, NPs have be loaded into biocompatible gels to form nanocomposite hybrid material (polysaccharide hydrogels containing magnetic NPs) that can be further conjugated with anticancer drugs to allow their release close to the target. The organic part of hybrid biomaterials is a carboxymethylcellulose (CMC) polymer, while the inorganic part consists of CoFe2O4 NPs coated with (3-aminopropyl)trimethoxysilane. The biological activity of these hybrid hydrogels was evaluated in vitro and in vivo. Our findings showed that hybrid hydrogels, instead of NPs alone, were not toxic on endothelial, stromal and epithelial cells, safe and biodegradable in vivo. In conclusion, biohydrogels with paramagnetic NPs as cross-linkers can be further exploited for antitumor drug loading and delivery systems.

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Biohydrogels with magnetic nanoparticles as crosslinker: Characteristics and potential use for controlled antitumor drug-delivery

Cited by 67 publications

References 35 publications

Design and Optimization of PLGA-Based Diclofenac Loaded Nanoparticles

Design and Optimization of PLGA-Based Diclofenac Loaded Nanoparticles

Influence of Alternating and Static Magnetic Fields on Drug Release from Hybrid Hydrogels Containing Magnetic Nanoparticles

Monitoring Endothelial and Tissue Responses to Cobalt Ferrite Nanoparticles and Hybrid Hydrogels

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