Biohybrid Response Microparticles Decorated with Trained‐MSCs for Acute Liver Failure Recovery

Abstract

Microcarrier-based mesenchymal stem cells (MSCs) delivery have attracted increasing attention in acute liver failure (ALF) therapy, while there is still room for improvement in terms of improving cell loading efficiency, enhancing anti-inflammatory features, and controlling cell release. Here, novel lipopolysaccharide (LPS)-composited magnetic-thermal responsive inverse opal particles (MIOPs) are presented for the delivery of MSCs. The MIOPs are composed of a chitosan inverse opal skeleton filled with a hydrogel containing LPS, poly(N-isopropylacrylamide), and Fe 3 O 4 nanoparticles. Benefitting from the biocompatible chitosan component and the huge specific surface area, the resultant MIOPs can capture MSCs in a nondestructive way. Furthermore, LPS can be released from the MIOPs under the stimulation of an alternating magnetic field, by which the MSCs are activated to gain the feature of "trained immunity." Moreover, this process can be monitored in real-time by the structural color change of the MIOPs. With that, the MSCs-laden MIOPs are employed in rats with ALF, and they exhibit obvious anti-inflammatory and therapeutic efficacy superior to untrained MSCs. These performances make the MIOPs a distinctive cell delivery platform for clinical tissue recovery applications.

Ultrasound-responsive matters for biomedical applications

“…Since PFC liquid features a low boiling point, microfluidic technology allows a moderate fabrication process, thus realizing the maintenance of the physiochemical properties of PFC. 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 Ascribed to the US-induced phase-transition ability of PFCs, the PO/GI-MCs experienced the transition from microcapsules to microbubbles when exposed to US waves (1.0 MHz, 1.2 W cm −2 ). Since the volume of PFCs can become five times larger, the resultant gas core splits the alginate shell, releasing the gas, which contains a large amount of oxygen.…”

Pollen‐Inspired Adhesive Multilobe Microparticles from Microfluidics for Intestinal Drug Delivery

“…[6][7][8][9][10][11] Among them, microparticles (MPs) with adjustable morphologies and functionalities have attracted tremendous attention in gastrointestinal drug administration. [11][12][13][14] Current studies have figured several methods to enhance the adhesive property of MPs, including the modulation of the MPs morphologies to increase the contact area, the addition of adhesive molecules like polydopamine, and so on. [6,7,11,14] Despite the achieved progresses, the adhesion and motion property of these MPs remain unsatisfied due to the unstable state of these adhesion molecules in the dynamic and moist physiological environment, such as the gastrointestinal tract.…”

Recent advances in liver‐on‐chips: Design, fabrication, and applications

“…18,19 Therefore, it has become an increasingly popular topic to establish more accurate and bionic in vitro liver models. [20][21][22] In recent years, to overcome the above-mentioned shortcomings, people have explored liver-on-chips, [23][24][25] which are novel liver cell culture platforms that mimic the key structure and core functions of the liver. The liveron-chips consist of real living human hepatocytes, and those commonly used include primary human cells, immortalized human cell lines, and others.…”