Biohybrid hematopoietic niche for expansion of hematopoietic stem/progenitor cells by using geometrically controlled fibrous layers

Batnyam, Onon; Shimizu, Harue; Saito, Koichi; Ishida, Tomohiko; Suye, Shin‐ichiro; Fujita, Shin

doi:10.1039/c5ra13332g

Cited by 18 publications

(9 citation statements)

References 33 publications

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“…This 3‐D biohybrid scaffold facilitated high‐density expansion of multipotent HSPC. The microscale architecture of the electrospun fiber scaffolds, more closely resembling natural ECM, together with its mechanical properties and ability to enhance expression of Jagged‐1in co‐cultured MSC (known to play a role in the self‐renewal of HSPC through Notch‐1 – in HSPC – and Jagged‐1 – in stromal cells – interactions), were suggested to be relevant features of the 3‐D system in comparison to TCPS‐cultured HSPC and MSC . Electrospun poly(lactic acid) (PLLA) nanofibrous scaffolds, seeded with niche‐like units extracted from mice BM, were also shown to be suitable for implantation in irradiated mice and to contribute to the restoration of their hematopoietic system .…”

Section: ‐D Co‐culture Of Hspc and Mscmentioning

confidence: 99%

Hematopoietic Niche – Exploring Biomimetic Cues to Improve the Functionality of Hematopoietic Stem/Progenitor Cells

Costa

Soure

Cabral

et al. 2017

Biotechnology Journal

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The adult bone marrow (BM) niche is a complex entity where a homeostatic hematopoietic system is maintained through a dynamic crosstalk between different cellular and non-cellular players. Signaling mechanisms triggered by cell-cell, cell-extracellular matrix (ECM), cell-cytokine interactions, and local microenvironment parameters are involved in controlling quiescence, self-renewal, differentiation, and migration of hematopoietic stem/progenitor cells (HSPC). A promising strategy to more efficiently expand HSPC numbers and tune their properties ex vivo is to mimic the hematopoietic niche through integration of adjuvant stromal cells, soluble cues, and/or biomaterial-based approaches in HSPC culture systems. Particularly, mesenchymal stem/stromal cells (MSC), through their paracrine activity or direct contact with HSPC, are thought to be a relevant niche player, positioning HSPC-MSC co-culture as a valuable platform to support the ex vivo expansion of hematopoietic progenitors. To improve the clinical outcome of hematopoietic cell transplantation (HCT), namely when the available HSPC are present in a limited number such is the case of HSPC collected from umbilical cord blood (UCB), ex vivo expansion of HSPC is required without eliminating the long-term repopulating capacity of more primitive HSC. Here, we will focus on depicting the characteristics of co-culture systems, as well as other bioengineering approaches to improve the functionality of HSPC ex vivo.

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Section: ‐D Co‐culture Of Hspc and Mscmentioning

confidence: 99%

Hematopoietic Niche – Exploring Biomimetic Cues to Improve the Functionality of Hematopoietic Stem/Progenitor Cells

Costa

Soure

Cabral

et al. 2017

Biotechnology Journal

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“…Previous studies have confirmed that 3D scaffolds could support the better maintenance of HSCs. However, if use MSCs or ECM protein as feeder layer, their consequences could be improved, because of cellcell interaction between CD34+ cells and feeder cells or ECM [16][17][18][19][20][21].…”

Section: Discussionmentioning

confidence: 99%

Potential of Polycaprolactone Nanofiber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells

Hadi¹,

Abroun²,

Soleimani³

et al. 2019

Int J Stem Cell Res Ther

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Background: An efficient and practical ex vivo expansion of cord blood hematopoietic stem cells as an alternative source of HSC transplantation is crucial in understanding the potential of HSC transplantation in treating or supportive therapy in a variety of hematologic and non-hematologic disorders. The aim of this study was an ex vivo expansion of cord blood hematopoietic stem cells in a novel threedimensional polycaprolactone nanofiber scaffold coated with collagen. Methods: After 10-day cultured of cord blood CD34+ cells in 2-Dimensional and 3-Dimensional culture system, the evaluation was performed by qRT-PCR, flow cytometry and clonogenicity. Results: 3-Dimensional Polycaprolactone nano-scaffold coated with collagen provided higher total nucleated cells (50-fold vs. 38-fold) and CD34+ cells (20-fold vs. 2.6-fold) (p < 0.05) and compared to 2-Dimensional cell culture and before expansion had higher expression of homing and self-renewal genes and for VLA-4, hTERT and BMI-1 genes were statically significant (p = 0.0001). The expression of myeloid markers in 3-Dimensional scaffold was significantly higher than the 2-Dimensional culture system (p < 0.05). The total colony in 2-Dimensional culture was lower than 3-Dimensional culture medium (p < 0.05). Conclusion: This study demonstrated the synergistic effect between the three-dimensionality of the scaffold and collagen as an extracellular matrix protein, and the potential of this 3-Dimensional Polycaprolactone nanofiber scaffold coated with collagen for ex vivo expansion of HSCs.

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“…However, it is relatively easy to obtain HSPCs from umbilical cord blood (UCB); such cells proliferate better than BM-and PB-derived cells. As might be expected, the number of cells available from a single umbilical cord is limited [4][5][6][7]. Ex vivo expansion of HSPCs is thus essential.…”

Section: Introductionmentioning

confidence: 99%

“…However, cross-contamination is possible and long-term engraftment is poor. Thus, many researchers have constructed three-dimensional (3D) environments mimicking the in vivo niches of HSPCs [10][11][12] in an effort to enhance cell attachment to structural supports and enable cell-cell and cell-matrix interactions, thus improving cellular potential [6,13,14]. Therefore, when functional ex vivo expansion of HSPCs is essential, the 3 D culture conditions should first be considered.…”

Section: Introductionmentioning

confidence: 99%

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The combined effects of hierarchical scaffolds and mechanical stimuli on ex vivo expansion of haematopoietic stem/progenitor cells

Kim

Lee

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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We describe the ex vivo expansion of haematopoietic stem/progenitor cells (HSPCs) with consideration of their eventual in-vivo niche. We firstly fabricated hierarchically structured scaffolds (lattices derived via three-dimensional plotting combined with electrospun submicron fibers coated with vitronectin to increase cell affinity). We also applied intermittent hydrostatic pressure (IHP) to mimic the physical environment of the in vivo niche. In the absence of mechanical stimuli, the cell phenotype (CD34 þ , CD34 þ CD38 -) remained excellent in the vitronectin-treated group. Two IHP regimens were tested; optimally, cells were pressurized (20 kPa) for 2 min and then rested for 13 min. On day 7 of culture, the total cell number had increased 21.2-fold and that of CD34 þ cells 10.94-fold. CD34 þ and CD34 þ CD38cells constituted 44.50 and 44.07% of total cells, respectively. Colony-forming counts and the long-term culture-initiating cell assay showed that clonogenic potential was greatly improved under our experimental conditions. Scaffolds with hierarchical structures were valuable in this context. Furthermore, ex vivo expansion of HSPCs was improved by physical stimulation. ARTICLE HISTORY

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Biohybrid hematopoietic niche for expansion of hematopoietic stem/progenitor cells by using geometrically controlled fibrous layers

Abstract: Biohybrid hematopoietic niche for expansion of hematopoietic stem/progenitor cells.

Cited by 18 publications

References 33 publications

Hematopoietic Niche – Exploring Biomimetic Cues to Improve the Functionality of Hematopoietic Stem/Progenitor Cells

Hematopoietic Niche – Exploring Biomimetic Cues to Improve the Functionality of Hematopoietic Stem/Progenitor Cells

Potential of Polycaprolactone Nanofiber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells

The combined effects of hierarchical scaffolds and mechanical stimuli on ex vivo expansion of haematopoietic stem/progenitor cells

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