Biohybrid fluorescent nanodiamonds as dual‐contrast markers for light and electron microscopies

Sotoma, Shingo; Hsieh, Feng-Jen; Chang, Huan‐Cheng

doi:10.1002/jccs.201800157

Cited by 15 publications

(17 citation statements)

References 98 publications

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“…Different techniques available for the development of graft copolymer synthesis differ basically in the mechanism used for generating free radical sites on the backbone polymer. [48,49] The developed substance was checked for magnetization by setting a magnet outside the glass vessel in which the developed substance is stored (Figure 1). The developed substances were attracted to the magnet, and accordingly, they were separated from the mixture within 30 seconds, with a clear solution left behind.…”

Section: Preparation Of Mnps and Nanocompositementioning

confidence: 99%

Poly(butyl methacrylate)‐grafted alginate/Fe₃O₄ nanocomposite: Synthesis and its application for removal of dyes

Alzahrani

Abo‐Dief

Algethami

2020

J Chinese Chemical Soc

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This study involved the utilization of a free radical-graft copolymerization reaction for the development of a novel adsorbent, namely, poly(butyl methacrylate)grafted alginate/Fe 3 O 4 nanocomposite (PBMA-gft-Alg/Fe 3 O 4). Transmission electron microscopy, scanning electron microscopy, energy-dispersive X-ray analysis, X-ray diffraction patterns analysis, and Fourier transform infrared spectroscopy (FT-IR) were carried out for the characterization of Fe 3 O 4 NPs and PBMA-gft-Alg/Fe 3 O 4 nanocomposites. The capability of nanocomposites and nanoparticles to adsorb dyes such as MG and MB, resulting in their removal from aqueous media, was evaluated under different conditions such as pH, temperature, contact time, and dose of adsorbent. Optimum parameters for adsorption of dyes were found to be pH of 10, 50 C, contact time of 180 min, and 0.2 g of adsorbent. Efficiency of the PBMA-gft-Alg/Fe 3 O 4 nanocomposite was found to be significantly greater than that of Fe 3 O 4 NPs for eliminating the desired dye. Langmuir, Freundlich, Sips, and Temkin models were used for testing the experimental data. Freundlich model was the one that best described the adsorption.

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Section: Preparation Of Mnps and Nanocompositementioning

confidence: 99%

Poly(butyl methacrylate)‐grafted alginate/Fe₃O₄ nanocomposite: Synthesis and its application for removal of dyes

Alzahrani

Abo‐Dief

Algethami

2020

J Chinese Chemical Soc

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“…Moreover, the platform is perfectly compatible with superresolution fluorescence imaging, cathodoluminescence imaging, and correlative light-electron microscopy by use of FNDs as photostable contrast agents and fiducial markers. 28,46,47 Further advancement of the technology is expected to facilitate diverse applications of these novel multifunctional nanoparticles in theranostics, nanomedicine, and related areas.…”

Section: ■ Conclusionmentioning

confidence: 99%

Intracellular Delivery of Luciferase with Fluorescent Nanodiamonds for Dual-Modality Imaging of Human Stem Cells

Lin

et al. 2019

Bioconjugate Chem.

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Delivering functional proteins (such as enzymes) into cells is important in various biological studies and is often accomplished indirectly by transfection with DNA or mRNA encoding recombinant proteins. However, the transfection efficiency of conventional plasmid methods is low for primary cells, which are crucial sources of cell therapy. Here, we present a new platform based on the use of fluorescent nanodiamond (FND) as a biocompatible nanocarrier to enable rapid, effective, and homogeneous labeling of human mesenchymal stem cells (MSCs) with luciferase for multiplex assays and ultrasensitive detection. More than 100 pg of FND and 100 million copies of firefly luciferase can be delivered into each MSC through endocytosis. Moreover, these endocytic luciferase molecules are catalytically active for hours, allowing the cells to be imaged and tracked in vitro as well as in vivo by both fluorescence and bioluminescence imaging. Our results demonstrate that luciferase-conjugated FNDs are useful as multifunctional labels of human stem cells for diverse theranostic applications.

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“…In contrast to Qdots, no blinking is observed. Modification of the surface is also relatively easy (Sotoma et al, 2018); e.g., Tsai et al successfully used this advantage to attach gold nanorods as tiny heat sources onto the surface of nanodiamonds to probe the local temperature at the heated dot (Tsai et al, 2017). Biocompatibility has been shown to be good.…”

Section: Bright Nano-dots As Single Probes For Trackingmentioning

confidence: 99%

Bright Dots and Smart Optical Microscopy to Probe Intracellular Events in Single Cells

Fujita

Zhong

Arai

et al. 2019

Front. Bioeng. Biotechnol.

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Probing intracellular events is a key step in developing new biomedical methodologies. Optical microscopy has been one of the best options to observe biological samples at single cell and sub-cellular resolutions. Morphological changes are readily detectable in brightfield images. When stained with fluorescent molecules, distributions of intracellular organelles, and biological molecules are made visible using fluorescence microscopes. In addition to these morphological views of cells, optical microscopy can reveal the chemical and physical status of defined intracellular spaces. This review begins with a brief overview of genetically encoded fluorescent probes and small fluorescent chemical dyes. Although these are the most common approaches, probing is also made possible by using tiny materials that are incorporated into cells. When these tiny materials emit enough photons, it is possible to draw conclusions about the environment in which the tiny material resides. Recent advances in these tiny but sufficiently bright fluorescent materials are nextly reviewed to show their applications in tracking target molecules and in temperature imaging of intracellular spots. The last section of this review addresses purely optical methods for reading intracellular status without staining with probes. These non-labeling methods are especially essential when biospecimens are thereafter required for in vivo uses, such as in regenerative medicine.

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Biohybrid fluorescent nanodiamonds as dual‐contrast markers for light and electron microscopies

Cited by 15 publications

References 98 publications

Poly(butyl methacrylate)‐grafted alginate/Fe₃O₄ nanocomposite: Synthesis and its application for removal of dyes

Poly(butyl methacrylate)‐grafted alginate/Fe₃O₄ nanocomposite: Synthesis and its application for removal of dyes

Intracellular Delivery of Luciferase with Fluorescent Nanodiamonds for Dual-Modality Imaging of Human Stem Cells

Bright Dots and Smart Optical Microscopy to Probe Intracellular Events in Single Cells

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Biohybrid fluorescent nanodiamonds as dual‐contrast markers for light and electron microscopies

Cited by 15 publications

References 98 publications

Poly(butyl methacrylate)‐grafted alginate/Fe3O4 nanocomposite: Synthesis and its application for removal of dyes

Poly(butyl methacrylate)‐grafted alginate/Fe3O4 nanocomposite: Synthesis and its application for removal of dyes

Intracellular Delivery of Luciferase with Fluorescent Nanodiamonds for Dual-Modality Imaging of Human Stem Cells

Bright Dots and Smart Optical Microscopy to Probe Intracellular Events in Single Cells

Contact Info

Product

Resources

About

Poly(butyl methacrylate)‐grafted alginate/Fe₃O₄ nanocomposite: Synthesis and its application for removal of dyes

Poly(butyl methacrylate)‐grafted alginate/Fe₃O₄ nanocomposite: Synthesis and its application for removal of dyes