Biohybrid cardiac ECM-based hydrogels improve long term cardiac function post myocardial infarction

Efraim, Yael; Sarig, Hadar; Anavy, Noa Cohen; Sarig, Udi; Berardinis, Elio de; Chaw, Su-Yin; Krishnamoorthi, Muthukumar; Kalifa, Jérôme; Bogireddi, Hanumakumar; Duc, Thang Vu; Kofidis, Theo; Baruch, Limor; Boey, Freddy Yin Chiang; Venkatraman, Subbu; Machluf, Marcelle

doi:10.1016/j.actbio.2016.12.015

Cited by 103 publications

(108 citation statements)

References 67 publications

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“…This review provides an overview of recent advances and hurdles that remain to be overcome in the biomaterial design and engineering of various injectable hydrogel systems, with emphasis placed on systems being used in cardiac tissue engineering efforts. The articles that comprise the focus of this review are shown in Table 1 …”

Section: Introductionmentioning

confidence: 99%

Injectable Hydrogels for Cardiac Tissue Engineering

Peña

Laughter

Jett

et al. 2018

Macromolecular Bioscience

185

168

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In light of the limited efficacy of current treatments for cardiac regeneration, tissue engineering approaches have been explored for their potential to provide mechanical support to injured cardiac tissues, deliver cardio-protective molecules, and improve cell-based therapeutic techniques. Injectable hydrogels are a particularly appealing system as they hold promise as a minimally invasive therapeutic approach. Moreover, injectable acellular alginate-based hydrogels have been tested clinically in patients with myocardial infarction (MI) and show preservation of the left ventricular (LV) indices and left ventricular ejection fraction (LVEF). This review provides an overview of recent developments that have occurred in the design and engineering of various injectable hydrogel systems for cardiac tissue engineering efforts, including a comparison of natural versus synthetic systems with emphasis on the ideal characteristics for biomimetic cardiac materials.

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Section: Introductionmentioning

confidence: 99%

Injectable Hydrogels for Cardiac Tissue Engineering

Peña

Laughter

Jett

et al. 2018

Macromolecular Bioscience

185

168

View full text Add to dashboard Cite

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“…In addition, due to its biological nature, dECM is prone to remodeling and degradation by resident or infiltrating cells in vivo, reducing even further its stability. Therefore, a series of studies have developed composite dECM hydrogels, incorporating synthetic or natural components so as to increase the construct durability and mechanical properties . While crosslinking was shown by many studies to substantially improve the construct's mechanical stability and retention time, as will be demonstrated further, it poses some challenges which could hinder the therapeutic potential of dECM.…”

Section: Decm Processing Approachesmentioning

confidence: 99%

“…j) The modification of cardiac‐derived dECM hydrogel with different ratios of genipin and chitosan has improved hydrogel mechanical properties and k) stimulated an improved ventricular performance of infarcted rat hearts. Reproduced with permission . Copyright 2016, Elsevier.…”

Section: Applications Of Processed Decm‐based Materialsmentioning

confidence: 99%

“…While offering numerous important advantages, processed dECM generates relatively soft hydrogels, impeding their in vivo long‐term retention and prolonged recruitment of host progenitor cells. Tuning the mechanical properties of dECM hydrogels can be obtained through its blending and/or crosslinking with different natural or synthetic polymers, resulting in increased stiffness and lower degradation rates . For example, cardiac dECM hydrogel can be formulated with genipin, a naturally occurring crosslinking agent, and chitosan, a natural linear polysaccharide, both of which are biocompatible and are commonly used in tissue engineering applications, leading to impressive improvements of cardiac function in chronic and acute myocardial infarction models (Figure j,k) .…”

Section: Applications Of Processed Decm‐based Materialsmentioning

confidence: 99%

“…The use of tissue‐derived decellularized extracellular matrices (dECM) has been dated back to the beginning of the 20th century, and was since followed and improved by an increasing number of groups revealing the endless potential of this unique biomaterial, isolated from the heart, liver, pancreas, brain, bone, small intestinal submucosa, and urinary bladder, among other tissues . Numerous dECM‐based products have been successfully translated into the clinic for a myriad of medical applications, as has been excellently reviewed elsewhere …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Processed Tissue–Derived Extracellular Matrices: Tailored Platforms Empowering Diverse Therapeutic Applications

Krishtul

Baruch

Machluf

2019

Adv Funct Materials

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Tissue-derived decellularized extracellular matrices (dECM) have gradually become the gold standard of scaffolds for tissue engineering, owing to their close mirroring of the intricate composition, architecture, and topology of the native extracellular matrix (ECM). Intriguingly, further manipulation of these acellular tissues through various processing techniques has been demonstrated to be an effective strategy to control their characteristics and impart them with ample valuable new traits, thereby expanding their applicability to a significantly wider spectrum of research and translational applications. Herein, state-of-the-art processed dECM platforms and their potential applications are focused on. The ECM characteristics that make it so appealing for tissue engineering are presented, followed by a concise discussion on the main considerations for choosing a dECM source for such applications. The key methodologies for dECM processing, including hydrogel production, bioprinting, electrospinning, and production of porous scaffolds, microcarriers, and microcapsules, as well as their inherent advantages and challenges, are introduced. To demonstrate the use of processed dECM platforms for tissue engineering, selected in vivo and in vitro applications recently developed utilizing these platforms are highlighted. Finally, concluding remarks and a prospective outlook for future developments and improvements in the field of processed dECM-based devices are given.

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Drawing Inspiration from Developmental Biology for Cardiac Tissue Engineers

et al. 2021

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A sound understanding of developmental biology is part of the foundation of effective stem cell‐derived tissue engineering. Here, the key concepts of cardiac development that are successfully applied in a bioinspired approach to growing engineered cardiac tissues, are reviewed. The native cardiac milieu is studied extensively from embryonic to adult phenotypes, as it provides a resource of factors, mechanisms, and protocols to consider when working toward establishing living tissues in vitro. It begins with the various cell types that constitute the cardiac tissue. It is discussed how myocytes interact with other cell types and their microenvironment and how they change over time from the embryonic to the adult states, with a view on how such changes affect the tissue function and may be used in engineered tissue models. Key embryonic signaling pathways that have been leveraged in the design of culture media and differentiation protocols are presented. The cellular microenvironment, from extracellular matrix chemical and physical properties, to the dynamic mechanical and electrical forces that are exerted on tissues is explored. It is shown that how such microenvironmental factors can inform the design of biomaterials, scaffolds, stimulation bioreactors, and maturation readouts, and suggest considerations for ongoing biomimetic advancement of engineered cardiac tissues and regeneration strategies for the future.

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Biohybrid cardiac ECM-based hydrogels improve long term cardiac function post myocardial infarction

Cited by 103 publications

References 67 publications

Injectable Hydrogels for Cardiac Tissue Engineering

Injectable Hydrogels for Cardiac Tissue Engineering

Processed Tissue–Derived Extracellular Matrices: Tailored Platforms Empowering Diverse Therapeutic Applications

Drawing Inspiration from Developmental Biology for Cardiac Tissue Engineers

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