Bioglass Activated Skin Tissue Engineering Constructs for Wound Healing

Abstract

Wound healing is a complicated process, and fibroblast is a major cell type that participates in the process. Recent studies have shown that bioglass (BG) can stimulate fibroblasts to secrete a multitude of growth factors that are critical for wound healing. Therefore, we hypothesize that BG can stimulate fibroblasts to have a higher bioactivity by secreting more bioactive growth factors and proteins as compared to untreated fibroblasts, and we aim to construct a bioactive skin tissue engineering graft for wound healing by using BG activated fibroblast sheet. Thus, the effects of BG on fibroblast behaviors were studied, and the bioactive skin tissue engineering grafts containing BG activated fibroblasts were applied to repair the full skin lesions on nude mouse. Results showed that BG stimulated fibroblasts to express some critical growth factors and important proteins including vascular endothelial growth factor, basic fibroblast growth factor, epidermal growth factor, collagen I, and fibronectin. In vivo results revealed that fibroblasts in the bioactive skin tissue engineering grafts migrated into wound bed, and the migration ability of fibroblasts was stimulated by BG. In addition, the bioactive BG activated fibroblast skin tissue engineering grafts could largely increase the blood vessel formation, enhance the production of collagen I, and stimulate the differentiation of fibroblasts into myofibroblasts in the wound site, which would finally accelerate wound healing. This study demonstrates that the BG activated skin tissue engineering grafts contain more critical growth factors and extracellular matrix proteins that are beneficial for wound healing as compared to untreated fibroblast cell sheets.

Ion Therapy: A Novel Strategy for Acute Myocardial Infarction

“…[10,36,37] However, it is unknown whether the bioactive function of these local applied biomaterials can be utilized for systemic application such as AMI treatment through intravenous injection of material derived bioactive ions. [10,36,37] However, it is unknown whether the bioactive function of these local applied biomaterials can be utilized for systemic application such as AMI treatment through intravenous injection of material derived bioactive ions.…”

A mini review focused on the proangiogenic role of silicate ions released from silicon-containing biomaterials

“…When the Bioglass with fibroblast cell sheets were implanted to the wound site in rat, a successful wound healing was achieved at 14 days (Figure 4(a)). 56 The wound healing and new blood vessel formation during 14 days in skin defect were higher in the Bioglass group than in the control. It was suggested that the release of ions from Bioglass significantly activated fibroblast cells to secrete proangiogenic factors such as VEGF, bFGF, and epidermal growth factor (EGF).…”

Biomaterials and Nanotherapeutics for Enhancing Skin Wound Healing

“…These poly-thioketal urethane-based scaffolds were stable for 25 weeks in aqueous conditions but were degraded by tissue ROS in 7 weeks resulting in enhanced wound closure compared to polyester urethane dressings (Martin et al, 2014). Biomaterials based on bioglasses have recently also shown potential in enhancing wound healing and angiogenesis in animal model of wound healing (Mao et al, 2015; Xu et al, 2015; Zhao et al, 2015; Yu et al, 2016; Zhou et al, 2016). For instance, copper-doped borate bioactive glass microfibers were shown to increase both the rate of collagen deposition and angiogenesis in full-thickness wounds in rats (Zhao et al, 2015).…”