Bioglass 45S5: Structural characterization of short range order and analysis of biocompatibility with adipose-derived mesenchymal stromal cells in vitro and in vivo

Rodrigues, Cristiano; Naasani, Liliana Ivet Sous; Zanatelli, Carla; Paim, Thaís Casagrande; Azevedo, Jéssica Gonçalves; Lima, J.C. de; Fernandes, Marilda da Cruz; Buchner, Silvio; Wink, Márcia Rosângela

doi:10.1016/j.msec.2019.109781

Cited by 14 publications

(13 citation statements)

References 48 publications

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“…The final confirmation of the ZrO 2 ‐BAG biological properties was shown by an in vivo study. In this in vivo experiment, varying degrees of inflammatory reactions, including foreign body multinucleated giant cells near the BAG particles, were identified, a finding in concurrence with the results of previous studies showing a moderate inflammatory reaction in subcutaneous tissue with 45S5 BAG discs implanted during in vivo experiments 61,62 . After implantation, an inflammatory reaction was observed; this observation is often related to the reaction of the tissue with the BAG interface.…”

Section: Discussionsupporting

confidence: 90%

“…The improvement of angiogenesis enhances bone repair by transporting oxygen, nutrients, and soluble materials to local tissues. 65 Many authors have reported that various composition of BAG having a proangiogenic potential in animal model 61,62,65,66 . This was also confirmed in this study, as results revealed that the Zr‐BAGs supported blood vessels formation.…”

Section: Discussionsupporting

confidence: 88%

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Improvement of the mechanical and biological properties of bioactive glasses by the addition of zirconium oxide (ZrO₂) as a synthetic bone graft substitute

Kang

Seo

Ryu

et al. 2020

J Biomedical Materials Res

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In this study, mechanical properties of bioactive glass (BAG) synthetic bone graft substitute was improved by containing ZrO2 (ZrO2‐BAG), while maintaining advantageous biological properties of BAG such as osteoinductive and osteoconductive properties. The ZrO2‐BAG was produced by adding ZrO2 in the following proportions to replace Na2O in 45S5 BAG: 1% (Zr1‐BAG), 3% (Zr3‐BAG), 6% (Zr6‐BAG), and 12% (Zr12‐BAG). Properties including XRD, XPS, SEM, DSC, fracture toughness, and Vickers microhardness were evaluated. To assess the biological properties, Ca/P apatite formation, ion release, degradation rate, cell proliferation, ALP activity (ALP), and alizarin red S staining assay (ARS) were evaluated. Also, expression of osteogenic differentiation markers, Osteopontin (OPN), confirmed by immunofluorescence staining. Finally, an in vivo test was carried out to by implanting ZrO2‐BAG into the subcutaneous tissue of rats. The results of each test were statistically analyzed with one‐way ANOVA followed by Tukey's post hoc statistical test. Amorphous ZrO2‐BAG was successfully produced with increased mechanical properties as the ZrO2 content was increased. Additionally, ZrO2‐BAG exhibited a slower ion release and degradation rate compare to BAG without ZrO2. Bioactivity of ZrO2‐BAG was confirmed with apatite layer formed on the surface, significantly higher proliferation rate and significantly enhanced ALP and the degree of ARS of the cells compare to respective controls. The tissue reactions observed in the in vivo study showed neo‐formed vessels after implantation of ZrO2‐BAG.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Improvement of the mechanical and biological properties of bioactive glasses by the addition of zirconium oxide (ZrO₂) as a synthetic bone graft substitute

Kang

Seo

Ryu

et al. 2020

J Biomedical Materials Res

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“…Indeed, a low spreading during cell adhesion was detected associated with an increase of HA depositions around the cells that looks like differentiated osteocytes. Any apparent local or systemic toxicity for organs, or strong immunogenic reactions were noted, except a vascularized dense capsule around the graft 28 .…”

Section: Normalization Of Gene Expression Performed Using the Amount Expressed By Bm-mscs At D1mentioning

confidence: 99%

“…In our study we chose to use nude mice to limit the immune response against the MSC allograft. The MSC allograft in Balb-c mice is possible, but the capsule formation surrounding the graft, probably due to the immune response in immunocompetent mice, could be a complication for implants [28][29][30] . The pH and the calcium concentration can also affect (which was not certified by peer review) is the author/funder.…”

Section: Normalization Of Gene Expression Performed Using the Amount Expressed By Bm-mscs At D1mentioning

confidence: 99%

A comparative in vitro and in vivo study of osteogenicity by using two biomaterials and two human mesenchymal stem cell subtypes

Fiévet

Serratrice

Brulin

et al. 2021

Preprint

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Bone repair induced by stem cells and biomaterials may represent an alternative to autologous bone grafting. Here, we compared the efficiency of two biomaterials - biphasic calcium phosphate (BCP) and bioactive glass (BG) - when loaded with either adult bone marrow mesenchymal stem cells (BM-MSCs) or newborn nasal ecto-mesenchymal stem cells (NE-MSCs), the latter being collected for further repair of lip cleft-associated bone loss. Both cell types display the typical stem cell surface markers CD73+/CD90+/CD105+/nestin, and exhibit the MSC-associated osteogenic, chondrogenic and adipogenic multipotency. NE-MSCs produce less collagen and alkaline phosphatase than BM-MSCs. At the transcript level, NE-MSCs express more abundantly three genes coding for bone sialoprotein, osteocalcin and osteopontin, while BM-MSCs produce extra copies of RUNX2. BM-MSCs and NE-MSCs adhere and survive on BCP and BG. In vivo experiments reveal that bone formation is only observed with BM-MSCs transplanted on BCP biomaterial.

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“…Bioactive glass (BG) is a biomaterial based on SiO 2 , Na 2 O, CaO, and P 2 O 5 [5]. BG is rich in calcium and phosphorus, the main inorganic components of bones and teeth.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Glass as a Nanoporous Drug Delivery System for Teicoplanin

et al. 2020

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Featured Application: Bioactive glass proposed in this study demonstrated its possibility as a drug delivery system based on its nanoporous structure and bioactivity for biomedical application.Abstract: Bioactive glass (BG) was made by the sol-gel method and doped with boron (B) to increase its bioactivity. Microstructures of BG and B-doped BG were observed by scanning electron microscopy, and phase identification was performed using an X-ray diffraction diffractometer. The ion concentrations released after soaking in simulated body fluid (SBF) for 1, 4, and 7 days were measured by inductively coupled plasma mass spectrometry, and the pH value of the SBF was measured after soaking samples to determine the variation in the environment. Brunauer-Emmett-Teller (BET) analysis was performed to further verify the characteristics of mesoporous structures. High performance liquid chromatography was used to evaluate the drug delivery ability of teicoplanin. Results demonstrated that B-doped BG performed significantly better than BG in parameters assessed by the BET analysis. B-doped BG has nanopores and more rough structures, which is advantageous for drug delivery as there are more porous structures available for drug adsorption. Moreover, B-doped BG was shown to be effective for keeping pH values stable and releasing B ions during soaking in SBF. The cumulative release of teicoplanin from BG and B-doped BG reached 20.09% and 3.17% on the first day, respectively. The drug release gradually slowed, reaching 29.43% and 4.83% after 7 days, respectively. The results demonstrate that the proposed bioactive glass has potential as a drug delivery system.

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Bioglass 45S5: Structural characterization of short range order and analysis of biocompatibility with adipose-derived mesenchymal stromal cells in vitro and in vivo

Cited by 14 publications

References 48 publications

Improvement of the mechanical and biological properties of bioactive glasses by the addition of zirconium oxide (ZrO₂) as a synthetic bone graft substitute

Improvement of the mechanical and biological properties of bioactive glasses by the addition of zirconium oxide (ZrO₂) as a synthetic bone graft substitute

A comparative in vitro and in vivo study of osteogenicity by using two biomaterials and two human mesenchymal stem cell subtypes

Bioactive Glass as a Nanoporous Drug Delivery System for Teicoplanin

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Bioglass 45S5: Structural characterization of short range order and analysis of biocompatibility with adipose-derived mesenchymal stromal cells in vitro and in vivo

Cited by 14 publications

References 48 publications

Improvement of the mechanical and biological properties of bioactive glasses by the addition of zirconium oxide (ZrO2) as a synthetic bone graft substitute

Improvement of the mechanical and biological properties of bioactive glasses by the addition of zirconium oxide (ZrO2) as a synthetic bone graft substitute

A comparative in vitro and in vivo study of osteogenicity by using two biomaterials and two human mesenchymal stem cell subtypes

Bioactive Glass as a Nanoporous Drug Delivery System for Teicoplanin

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Product

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Improvement of the mechanical and biological properties of bioactive glasses by the addition of zirconium oxide (ZrO₂) as a synthetic bone graft substitute

Improvement of the mechanical and biological properties of bioactive glasses by the addition of zirconium oxide (ZrO₂) as a synthetic bone graft substitute