Biogeography of symbiotic and other endophytic bacteria isolated from medicinal Glycyrrhiza species in China

Li, Li; Sinkko, Hanna; Montonen, Leone; Wei, Gehong; Lindström, Kristina; Rásänen, Leena

doi:10.1111/j.1574-6941.2011.01198.x

Cited by 100 publications

(69 citation statements)

References 72 publications

(115 reference statements)

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“…Mesorhizobia show a high variability and capacity to adapt to local and extreme edaphic conditions that are site specific. This agrees with data revealing taxon-area biogeography within mesorhizobia symbiotic to, for instance, chickpea (54) and liquorice (55) legumes, as well as extensive species diversification with the root-nodulating members of the genus Mesorhizobium. Therefore, to remediate metal contamination, this work suggests that native plants and microorganisms that are better adapted to local pedoclimatic conditions should be used in phytostabilization strategies based on Mesorhizobium-legume interactions.…”

Section: Discussionsupporting

confidence: 81%

Ancient Heavy Metal Contamination in Soils as a Driver of Tolerant Anthyllis vulneraria Rhizobial Communities

Mohamad

Maynaud

Quéré

et al. 2017

Appl Environ Microbiol

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Anthyllis vulneraria is a legume associated with nitrogen-fixing rhizobia that together offer an adapted biological material for mine-soil phytostabilization by limiting metal pollution. To find rhizobia associated with Anthyllis at a given site, we evaluated the genetic and phenotypic properties of a collection of 137 rhizobia recovered from soils presenting contrasting metal levels. Zn-Pb mine soils largely contained metal-tolerant rhizobia belonging to Mesorhizobium metallidurans or to another sister metal-tolerant species. All of the metal-tolerant isolates harbored the cadA marker gene (encoding a metal-efflux P IB -type ATPase transporter). In contrast, metalsensitive strains were taxonomically distinct from metal-tolerant populations and consisted of new Mesorhizobium genospecies. Based on the symbiotic nodA marker, the populations comprise two symbiovar assemblages (potentially related to Anthyllis or Lotus host preferences) according to soil geographic locations but independently of metal content. Multivariate analysis showed that soil Pb and Cd concentrations differentially impacted the rhizobial communities and that a rhizobial community found in one geographically distant site was highly divergent from the others. In conclusion, heavy metal levels in soils drive the taxonomic composition of Anthyllis-associated rhizobial populations according to their metal-tolerance phenotype but not their symbiotic nodA diversity. In addition to heavy metals, local soil physicochemical and topoclimatic conditions also impact the rhizobial beta diversity. Mesorhizobium communities were locally adapted and site specific, and their use is recommended for the success of phytostabilization strategies based on Mesorhizobium-legume vegetation.IMPORTANCE Phytostabilization of toxic mine spoils limits heavy metal dispersion and environmental pollution by establishing a sustainable plant cover. This ecofriendly method is facilitated by the use of selected and adapted cover crop legumes living in symbiosis with rhizobia that can stimulate plant growth naturally through biological nitrogen fixation. We studied microsymbiont partners of a metaltolerant legume, Anthyllis vulneraria, which is tolerant to very highly metal-polluted soils in mining and nonmining sites. Site-specific rhizobial communities were linked to taxonomic composition and metal tolerance capacity. The rhizobial species Mesorhizobium metallidurans was dominant in all Zn-Pb mines but one. It was not detected in unpolluted sites where other distinct Mesorhizobium species occur. Given the different soil conditions at the respective mining sites, including their heavymetal contamination, revegetation strategies based on rhizobia adapting to local conditions are more likely to succeed over the long term compared to strategies based on introducing less-well-adapted strains.

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Section: Discussionsupporting

confidence: 81%

Ancient Heavy Metal Contamination in Soils as a Driver of Tolerant Anthyllis vulneraria Rhizobial Communities

Mohamad

Maynaud

Quéré

et al. 2017

Appl Environ Microbiol

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“…Our results from clonal inoculations corroborate the results from previous work, which found no effect of nonnodulating strains on legume host growth in isolation (14,25,45). However, under parameters that model relative abundances of nonnodulating strains within the rhizosphere, our data set revealed substantial costs to host growth and symbiont fitness in both host populations examined (except for total nodule biomass at BMR) ( Fig.…”

Section: Discussionsupporting

confidence: 82%

Nonnodulating Bradyrhizobium spp. Modulate the Benefits of Legume-Rhizobium Mutualism

Gano-Cohen

Stokes

Blanton

et al. 2016

Appl Environ Microbiol

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Rhizobia are best known for nodulating legume roots and fixing atmospheric nitrogen for the host in exchange for photosynthates. However, the majority of the diverse strains of rhizobia do not form nodules on legumes, often because they lack key loci that are needed to induce nodulation. Nonnodulating rhizobia are robust heterotrophs that can persist in bulk soil, thrive in the rhizosphere, or colonize roots as endophytes, but their role in the legume-rhizobium mutualism remains unclear. Here, we investigated the effects of nonnodulating strains on the native Acmispon-Bradyrhizobium mutualism. To examine the effects on both host performance and symbiont fitness, we performed clonal inoculations of diverse nonnodulating Bradyrhizobium strains on Acmispon strigosus hosts and also coinoculated hosts with mixtures of sympatric nodulating and nonnodulating strains. In isolation, nonnodulating Bradyrhizobium strains did not affect plant performance. In most cases, coinoculation of nodulating and nonnodulating strains reduced host performance compared to that of hosts inoculated with only a symbiotic strain. However, coinoculation increased host performance only under one extreme experimental treatment. Nearly all estimates of nodulating strain fitness were reduced in the presence of nonnodulating strains. We discovered that nonnodulating strains were consistently capable of coinfecting legume nodules in the presence of nodulating strains but that the fitness effects of coinfection for hosts and symbionts were negligible. Our data suggest that nonnodulating strains most often attenuate the Acmispon-Bradyrhizobium mutualism and that this occurs via competitive interactions at the root-soil interface as opposed to in planta. IMPORTANCERhizobia are soil bacteria best known for their capacity to form root nodules on legume plants and enhance plant growth through nitrogen fixation. Yet, most rhizobia in soil do not have this capacity, and their effects on this symbiosis are poorly understood. We investigated the effects of diverse nonnodulating rhizobia on a native legume-rhizobium symbiosis. Nonnodulating strains did not affect plant growth in isolation. However, compared to inoculations with symbiotic rhizobia, coinoculations of symbiotic and nonnodulating strains often reduced plant and symbiont fitness. Coinoculation increased host performance only under one extreme treatment. Nonnodulating strains also invaded nodule interiors in the presence of nodulating strains, but this did not affect the fitness of either partner. Our data suggest that nonnodulating strains may be important competitors at the root-soil interface and that their capacity to attenuate this symbiosis should be considered in efforts to use rhizobia as biofertilizers. Rhizobia are heterotrophic soil bacteria with diverse lifestyles. Some rhizobial lineages have acquired the capacity to form nodules on legume roots and fix atmospheric nitrogen for these hosts (1). Nodulating rhizobia are attracted to flavonoids released by legumes. In response, t...

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“…S2). The three strains isolated from nodules of Astragalus mongholicus may be an opportunistic entry to the nodules as reported by Li et al (2012).…”

Section: Strain Ccbau 23380mentioning

confidence: 60%

Ensifer glycinis sp. nov., a rhizobial species associated with species of the genus Glycine

Yan

Sui

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Rhizobial strains from root nodules of Astragalus mongholicus and soybean (Glycine max) were characterized phylogenetically as members of the genus Ensifer (formerly named Sinorhizobium), based on 16S rRNA gene sequence comparisons. Results based upon concatenated sequence analysis of three housekeeping genes (recA, atpD and glnII, 93.8 % similarities to known species) and average nucleotide identity (ANI) values of whole genome sequence comparisons (ranging from 89.6 % to 83.4 % to Ensifer fredii and Ensifer saheli, respectively) indicated the distinct positions of these novel strains within the genus Ensifer. Phylogeny of symbiotic genes (nodC and nifH) of three novel strains clustered them with rhizobial species Ensifer fredii and Ensifer sojae, both isolated from nodules of Glycine max. Cross-nodulation tests showed that the representative strain CCBAU 23380 T could form root nodules with nitrogen fixation capability on Glycine soja, Albizia julibrissin, Vigna unguiculata and Cajanus cajan, but failed to nodulate Astragalus mongholicus, its original host legume. Strain CCBAU 23380 T formed inefficient nodules on G. max, and it did not contain 18 : 0, 18 : 1!7c 11-methyl or summed feature 1 fatty acids, which differed from other related strains. Failure to utilize malonic acid as a carbon source distinguished strain CCBAU 23380 T from the type strains of related species. The genome size of CCBAU 23380 T was 6.0 Mbp, comprising 5624 predicted genes with DNA G+C content of 62.4 mol%. Based on the results above, a novel species, Ensifer glycinis sp. nov., is proposed, with CCBAU 23380 T (=LMG 29231 T =HAMBI 3645 T ) as the type strain.

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Biogeography of symbiotic and other endophytic bacteria isolated from medicinal Glycyrrhiza species in China

Cited by 100 publications

References 72 publications

Ancient Heavy Metal Contamination in Soils as a Driver of Tolerant Anthyllis vulneraria Rhizobial Communities

Ancient Heavy Metal Contamination in Soils as a Driver of Tolerant Anthyllis vulneraria Rhizobial Communities

Nonnodulating Bradyrhizobium spp. Modulate the Benefits of Legume-Rhizobium Mutualism

Ensifer glycinis sp. nov., a rhizobial species associated with species of the genus Glycine

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