Biogeography of Brazilian populations of Panstrongylus megistus (Hemiptera, Reduviidae, Triatominae) based on molecular marker and paleo-vegetational data

Barbosa, Silvia Ermelinda; Belisário, Carlota Josefovicz; Souza, Rita C. M.; Paula, Alexandre Silva de; Linardi, Pedro Marcos; Romanha, Álvaro J.; Diotaiuti, Liléia

doi:10.1016/j.actatropica.2006.07.010

Cited by 15 publications

(12 citation statements)

References 13 publications

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“…RAPD markers have been used in various studies of triatomine population genetics (Garcia et al 1998, Jaramillo et al 2001, Dorn et al 2003, Calderón et al 2004, Borges et al 2005, Barbosa et al 2006. Using analysis of the similarity presented by RAPD profiles, Borges et al (2005) observed a clear separation of Triatoma brasiliensis from the Brazilian state of Ceará into three samples from sylvatic, peridomiciliary and domiciliary habitats.…”

Section: Discussionmentioning

confidence: 99%

Population dynamics of Triatoma vitticeps (Stål, 1859) in Itanhomi, Minas Gerais, Brazil

Souza¹,

Barbosa²,

Sonoda³

et al. 2008

Mem. Inst. Oswaldo Cruz

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(Galvão et al. 2003, Galvão & Angulo 2006, Costa et al. 2006, Costa & Felix 2007, Sandoval et al. 2007, Bérenger & Blanchet 2007, Martinez et al. 2007) and is a vector of Trypanosoma cruzi. Its geographical distribution is limited to the Brazilian states of Bahia, Espírito Santo, Minas Gerais (MG), and Rio de Janeiro (Galvão et al. 2003). Although considered to be of secondary importance in T. cruzi transmission to man, this species shows high rates of trypanosome infection (Santos et al. 1969a,c, Pinto et al. 1969, Barros et al. 1975, Sessa & Carias 1986, Dias et al. 1989, Gonçalves et al. 1998, Santos et al. 2005 and is often captured in man-made habitats, with frequent reports of adult bugs invading houses (Santos et al. 1969b, Ferreira et al. 1986, Sessa & Carias 1986, Dias et al. 1989, Gonçalves et al. 1998, possibly attracted by light (Ferreira et al. 1986 to T. cruzi are very high, especially in adult bugs captured indoors in Itahomi. However, Chagas disease is rare in the area, a prevalence of only 0.4% has been recorded as part of a national serological survey (Camargo et al. 1984). It is not known whether the low transmission of T. cruzi to man is due to characteristics of the vector (the long interval between feeding and defecation) or the strain of parasite involved.Among the available taxonomic marker techniques, morphometry of the head, random amplified polymorphism of DNA (RAPD) and isoenzyme analysis have been most used in triatomine population studies (Costa et al. 1997, Dujardin et al. 1998, Borges et al. 2000, Soares et al. 2001, Barbosa et al 2003. We used these three techniques to investigate T. vitticeps population dynamics in the Itanhomi district. MATERIALS AND METHODSStudy area -Itanhomi (19°11'15"S, 41°48'45"W) has an estimated population of 11,012 inhabitants. The urban and rural area comprise 93 localities. In 11 of the rural sites (Adão Daniel, Cabeceira do Guido, Francisco Inácio, Jesus Julião, Jose Horta, Jose Noberto, João Caboclo, Júlio Carlos, Liés Alves, Orides I, and Valico) the search for T. vitticeps was intensively performed in 2002 in domestic and peridomestic environments. The following year, sylvatic sampling was attempted in Cabeceira do Guido, particularly in a wooded area 100 m from a house where adult triatomines are periodically detected.Houses in Itanhomi in which T. vitticeps was sampled conform to a distinct pattern of construction, being built on stilts.The floors of these dwellings are wooden 15 Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 103(1), February 2008 and often have cracks that allow triatomines to pass in and out; the floor is a kind of roof for the open basement (classified as the peridomicile). Houses are built on stilt, providing shelter for domestic animals and storage of materials. Furthermore, 100-500 m from the houses there are remnants of Atlantic forest and rocks, constituting sylvatic habitat for the bugs. This peculiar characteristic of the locality is the proximity of the habitats.Insects -A pool of T. vitticeps captured in Itanhomi ...

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Section: Discussionmentioning

confidence: 99%

Population dynamics of Triatoma vitticeps (Stål, 1859) in Itanhomi, Minas Gerais, Brazil

Souza¹,

Barbosa²,

Sonoda³

et al. 2008

Mem. Inst. Oswaldo Cruz

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“…9,10 Studies on population diversity of P. megistus in different regions in Brazil reported distinct degrees of adaptation to domestic habitats. [11][12][13][14][15] In northeastern states such as Bahia, P. megistus is well adapted to domestic habitats, but its presence in sylvatic foci has not been proven. 11,12 However, in southeastern states, it can be found in sylvatic and domestic habitats.…”

Section: Introductionmentioning

confidence: 99%

Lack of Segregation between Two Species of Chagas Disease Vectors

Mota¹,

Lorenzo²

2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Triatoma infestans and Panstrongylus megistus are relevant Chagas disease vectors. An apparent segregation among these triatomine species inside human households was suggested to rely on mutual repellence between them. However, P. megistus and T. infestans show aggregation responses to chemical signals emitted by the other species. These findings do not rule out the possibility that stimuli other than chemical signals could mediate repellence when these species exploit shelters simultaneously. In the present study, we investigated how P. megistus and T. infestans exploit shelters in controlled laboratory conditions and how insect density and environmental illumination modulate this behavior. We evaluated whether these species aggregate inside shelters or mutually repel each other. Panstrongylus megistus and T. infestans show specific patterns of shelter exploitation, which are differentially affected by insect density and environment illumination. In particular, P. megistus is more sensitive to insect density than T. infestans, whereas T. infestans shows higher sensitivity to illumination than P. megistus. Nevertheless, these species exploit shelters randomly without any apparent repellence.

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“…In an evaluation of populations in the States of Bahia, Minas Gerais and Santa Catarina, Barbosa et al 48 observed that the occupation of artifi cial environments may be related to a greater effi ciency in resource utilization, presenting a demographic cline where the lowest fi tness was observed in SC. Additionally, genetic and paleogeographic studies have demonstrated that the most recent geological events may have acted differently on the various P. megistus populations, leading to the observed behavioral differences in these species 49 .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of natural foci of Panstrongylus megistus in a forest fragment in Porto Alegre, State of Rio Grande do Sul, Brazil

Junior

Viola

Lorosa

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction:Panstrongylus megistus is commonly found in wild environments of the State of Rio Grande do Sul, Brazil. The aim of this study was to characterize the network of refuges used by triatomine in a forest fragment of Porto Alegre and to identify Trypanosoma cruzi infection, associated hosts and the epidemiological importance of both hosts and triatomines. Methods: Techniques including the spool-and-line method and active searching (transects) were used to identify natural foci. Results: The food source for each triatomine was determined using the precipitin test, and the infection of marsupials was determined by xenodiagnosis. A total of 33 adults (domestic environment) and 27 nymphs (wild environment) of P. megistus were found in addition to 43 Didelphis albiventris specimens. The infection rates of triatomine adults, triatomine nymphs and opossums with T. cruzi I were 64%, 73% and 69%, respectively. Birds, rodents and opossums were the main resources used by triatomine. Conclusions: This work presents the fi rst characterization of a natural focus of P. megistus in Rio Grande do Sul. The natural characteristics of this focus and its implication in the transmission of T. cruzi are discussed.

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Biogeography of Brazilian populations of Panstrongylus megistus (Hemiptera, Reduviidae, Triatominae) based on molecular marker and paleo-vegetational data

Cited by 15 publications

References 13 publications

Population dynamics of Triatoma vitticeps (Stål, 1859) in Itanhomi, Minas Gerais, Brazil

Population dynamics of Triatoma vitticeps (Stål, 1859) in Itanhomi, Minas Gerais, Brazil

Lack of Segregation between Two Species of Chagas Disease Vectors

Evaluation of natural foci of Panstrongylus megistus in a forest fragment in Porto Alegre, State of Rio Grande do Sul, Brazil

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