Biogeographic study of the honey bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on mitochondrial DNA analyses

Stevanović, Jevrosima; Stanimirović, Zoran; Radaković, Milena; Kovačević, Sanja

doi:10.1134/s1022795410050145

Cited by 30 publications

(32 citation statements)

References 20 publications

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“…14, 15 and 16, on altitudes 777, 600 and 435 m, respectively) are probably the results of their better adaptation to low temperatures. Previously evidenced genetic and behavioural differences among different ecotypes of Serbian honey bees (Stanimirovic et al 2002(Stanimirovic et al , 2005aStevanovic et al 2010;Muñoz et al 2012) support the idea that bees from Southern and Eastern regions of Serbia (localities No. 14, 15 and 16) may have better adaptation abilities.…”

Section: Discussionmentioning

confidence: 76%

Characteristics of Nosema ceranae infection in Serbian honey bee colonies

Stevanović¹,

Simeunović²,

Gajić³

et al. 2013

Apidologie

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-The incidence, symptoms and consequences of Nosema ceranae infection were monitored in 200 honey bee colonies in Serbia over 5 years (2008)(2009)(2010)(2011)(2012) to reveal if they display characteristics described for nosemosis type C. Continual high frequency of N. ceranae-positive colonies was recorded within each study year, ranging from 73 to 98 %. A seasonal pattern in N. ceranae incidence was observed over 4 years (2008)(2009)(2010)(2011). Symptoms traditionally attributed to Nosema apis infection were observed in the majority of N. ceranaeinfected colonies, both among surviving and those that died. These symptoms could not serve as predictive markers for colony losses, as they were significantly more frequently observed among surviving colonies in 2010, 2011 and 2012. No clear association between N. ceranae infection and colony losses was affirmed neither during the winter nor during the summer season. Thus, N. ceranae infection in Serbian bees do not fit the characteristics for nosemosis type C.Nosema ceranae / Apis mellifera / nosemosis type C / faeces

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Section: Discussionmentioning

confidence: 76%

Characteristics of Nosema ceranae infection in Serbian honey bee colonies

Stevanović¹,

Simeunović²,

Gajić³

et al. 2013

Apidologie

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“…Specifically, amplification of COI and ND5 genes followed by digestion with NcoI/StyI and AluI, respectively, produce diagnostic patterns characteristic of A. m. macedonica (Bouga et al, 2005;Stevanovic et al, 2010). Additionally, there are differences between…”

Section: Cleaved Fragment Uncleaved Fragment Authorsmentioning

confidence: 99%

“…This assay is recommended for honey bee maternal identification. (Smith et al, 1997;Bouga et al, 2005;Ivanova et al, 2010;Stevanovic et al, 2010). These assays have proved to be useful for discriminating variation in some eastern…”

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confidence: 99%

Standard methods for characterising subspecies and ecotypes ofApis mellifera

Meixner

Pinto

Bouga

et al. 2013

Journal of Apicultural Research

199

195

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SummaryThe natural diversity of honey bees in Europe is eroding fast. A multitude of reasons lead to a loss of both genetic diversity and specific adaptations to local conditions. To preserve locally adapted bees through breeding efforts and to maintain regional strains in conservation areas, these valuable populations need to be identified.In this paper, we give an overview of methods that are currently available and used for recognition of honey bee subspecies and ecotypes, or that can be utilised to verify the genetic origin of colonies for breeding purposes. Beyond summarising details of morphometric, allozyme and DNA methods currently in use, we report recommendations with regard to strategies for sampling, and suggest methods for statistical data analysis. In particular, we emphasise the importance of reference data and consistency of methods between laboratories to yield comparable results. Métodos estándar para la caracterización de las subespecies y ecotipos de Apis mellifera ResumenLa diversidad natural de la abeja de la miel se está deteriorando rápidamente en Europa. Existen multitud de razones que conducen tanto a una pérdida de diversidad genética como de adaptaciones específicas a las condiciones locales. Se necesita identificar a estas valiosas poblaciones para preservar a las abejas adaptadas a nivel local, mediante esfuerzos para la mejora y el mantenimiento de variedades regionales en las áreas de conservación.En este artículo, realizamos una revisión general de los actuales métodos disponibles que se utilizan para la determinación de subespecies y ecotipos de abejas melíferas, o que pueden ser utilizados para verificar el origen genético de las colmenas seleccionadas con fines de cría.Además, resumimos las características de los métodos morfométricos, de aloenzimas y de ADN, realizamos recomendaciones con respecto a las estrategias de muestreo, y sugerimos métodos para el análisis estadístico de los datos. En particular, destacamos la importancia de los datos de referencia y la coherencia de los métodos entre laboratorios para producir resultados comparables.

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“…На основе ПЦР-ПДРФ-анализа генов COI и Nd5 мтДHK эндонуклеазами NcoI, StyI и AluI у пчел подви-да A. m. macedonica из Македонии [34] [34]. ПЦР-ПДРФ-анализ гена CYT B мтДHK эндонуклеазой BglII позволил дифференцировать европейские семьи пчел (эволюционные ветви М и С) от африканских (эволю-ционная ветвь А) [37], тогда как рестрикционный анализ гена Ls rRNA мтДHK эндонуклеазой HincII давал воз-можность дифференцировать семьи пчел, относящихся к эволюционным ветвям М и С [35].…”

Section: оценка таксономической принадлежности семей пчел на основе иunclassified

Modern methods of assessing the taxonomic affiliation of honeybee colonies

Il’yasov¹,

Poskryakov²,

Николенко³

2017

Ecological genetics

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At least 30 subspecies of the honeybee Apis mellifera L. were formed allopatrically during the evolution, which spreaded throughout all Africa, Europe and West Asia. The dark forest bee Apis mellifera mellifera is the only and most valuable subspecies for the Northern and Western Europe countries, adapted to productive living in the hard-continental climate of Eurasia. In the past 100 years, natural geographical isolation of subspecies has been disrupted as a result of a human activities. Mass transportations of honeybee colonies beyond the boundaries of their area have been threatened of loss the identity of gene pool of subspecies as a result of hybridization. Preservation of the gene pool of subspecies is possible only when controlling the transportation of honeybee colonies using the methods of identification of taxonomic affiliation of honeybee colonies. Now, dozens of methods have been developed to identify the taxonomic affiliation of honeybee's colony, which are based on the variability of body parts, allozyme loci, mitochondrial DNA loci, microsatellite nuclear loci, sites of single nucleotide polymorphism (SNP). The variability of microsatellite loci and the single nucleotide polymorphism sites have shown the greatest informativeness in identification of the taxonomic affiliation of honeybee's colony.

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Biogeographic study of the honey bee (Apis mellifera L.) from Serbia, Bosnia and Herzegovina and Republic of Macedonia Based on mitochondrial DNA analyses

Cited by 30 publications

References 20 publications

Characteristics of Nosema ceranae infection in Serbian honey bee colonies

Characteristics of Nosema ceranae infection in Serbian honey bee colonies

Standard methods for characterising subspecies and ecotypes ofApis mellifera

Modern methods of assessing the taxonomic affiliation of honeybee colonies

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