Biogeochemistry, microbial activity, and diversity in surface and subsurface deep‐sea sediments of South China Sea

Zhuang, Guang‐Chao; Liu, Xu; Liang, Qianyong; Fan, Xibei; Xia, Zhen; Joye, Samantha B.; Wang, Fengping

doi:10.1002/lno.11182

Cited by 36 publications

(38 citation statements)

References 99 publications

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“…ANME-2a/b is widely distributed in global sediments, and especially dominates in cold seep environments (Knittel et al, 2005;Biddle et al, 2012). A large amount of ANME-2a/b has been detected in cold seeps in the SCS (Niu et al, 2017;Cui et al, 2019;Zhuang et al, 2019). ANME-2a/b was found throughout the entire sediment core ROV07-01 in this study.…”

Section: Active Archaeal Anaerobic Methanotrophs In Cold Seepssupporting

confidence: 56%

“…Multiple studies have shown that ANMEs are dominant in cold seeps in the SCS (Niu et al, 2017;Cui et al, 2019;Zhuang et al, 2019). However, previous results were based on the DNA level, and thus inactive microorganisms may have been included (Luna et al, 2002;Ostle et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, ANME communities were investigated using highthroughput sequencing technology in the sediments of the South China Sea (SCS) (Niu et al, 2017;Cui et al, 2019;Zhuang et al, 2019). However, these studies only focused on the overall microbial communities at the DNA level, without distinguishing among active, dormant, and dead microbes.…”

Section: Introductionmentioning

confidence: 99%

“…), living tubeworms (Paraescarpia echinospica), and living mussels (Bathymodiolus plantifrons) (Feng and Chen, 2015;Liang et al, 2017). Large numbers of ANMEs have been found in sediments from Haima cold seeps through DNA investigation, including ANME-1a, ANME-1b, ANME-2a/b, and ANME-2c (Niu et al, 2017;Guan et al, 2018;Cui et al, 2019;Zhuang et al, 2019). In 2018, two weak seeps (ROV05 and ROV07, 5.22 km apart) were newly discovered using the Haima ROV.…”

Section: Introductionmentioning

confidence: 99%

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Active Anaerobic Archaeal Methanotrophs in Recently Emerged Cold Seeps of Northern South China Sea

et al. 2020

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Cold seep ecosystems are developed from methane-rich fluids in organic rich continental slopes, which are the source of various dense microbial and faunal populations. Extensive studies have been conducted on microbial populations in this unique environment; most of them were based on DNA, which could not resolve the activity of extant organisms. In this study, RNA and DNA analyses were performed to evaluate the active archaeal and bacterial communities and their network correlations, particularly those participating in the methane cycle at three sites of newly developed cold seeps in the northern South China Sea (nSCS). The results showed that both archaeal and bacterial communities were significantly different at the RNA and DNA levels, revealing a higher abundance of methane-metabolizing archaea and sulfate-reducing bacteria in RNA sequencing libraries. Site ROV07-01, which exhibited extensive accumulation of deceased Calyptogena clam shells, was highly developed, and showed diverse and active anaerobic archaeal methanotrophs (ANME)-2a/b and sulfate-reducing bacteria from RNA libraries. Site ROV07-02, located near carbonate crusts with few clam shell debris, appeared to be poorly developed, less anaerobic and less active. Site ROV05-02, colonized by living Calyptogena clams, could likely be intermediary between ROV07-01 and ROV07-02, showing abundant ANME-2dI and sulfate-reducing bacteria in RNA libraries. The high-proportions of ANME-2dI, with respect to ANME-2dII in the site ROV07-01 was the first report from nSCS, which could be associated with recently developed cold seeps. Both ANME-2dI and ANME-2a/b showed close networked relationships with sulfate-reducing bacteria; however, they were not associated with the same microbial operational taxonomic units (OTUs). Based on the geochemical gradients and the megafaunal settlements as well as the niche specificities and syntrophic relationships, ANMEs appeared to change in community structure with the evolution of cold seeps, which may be associated with the heterogeneity of their geochemical processes. This study enriched our understanding of more active sulfate-dependent anaerobic oxidation of methane (AOM) in poorly developed and active cold seep sediments by contrasting DNA- and RNA-derived community structure and activity indicators.

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Section: Active Archaeal Anaerobic Methanotrophs In Cold Seepssupporting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Active Anaerobic Archaeal Methanotrophs in Recently Emerged Cold Seeps of Northern South China Sea

et al. 2020

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“…It should be noted, however, that most of these studies focused solely on the Atlantic Ocean and mainly on shallow, aerobic sub‐surface waters, with only one study investigating a gradient of up to 500 m depth (Dixon and Nightingale, 2012). Estimates of methanol levels in coastal area sediments range between 0.3 μM and over 100 μM in environments as diverse as the Orca Basin in the Gulf Mexico, East Japan Sea and the South China Sea (Yanagawa et al ., 2016; Zhuang et al ., 2014; 2019a,b). In these studies, methanol concentrations were found to increase with depth, with the lowest concentrations close to the seafloor and the highest concentrations at depths of 10–20 m below the seafloor.…”

Section: Sources Of Methanol In Marine Sedimentsmentioning

confidence: 99%

Anaerobic microbial methanol conversion in marine sediments

Fischer

Sánchez‐Andrea

Stams

et al. 2021

Environmental Microbiology

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Methanol is an ubiquitous compound that plays a role in microbial processes as a carbon and energy source, intermediate in metabolic processes or as end product in fermentation. In anoxic environments, methanol can act as the sole carbon and energy source for several guilds of microorganisms: sulfatereducing microorganisms, nitrate-reducing microorganisms, acetogens and methanogens. In marine sediments, these guilds compete for methanol as their common substrate, employing different biochemical pathways. In this review, we will give an overview of current knowledge of the various ways in which methanol reaches marine sediments, the ecology of microorganisms capable of utilizing methanol and their metabolism. Furthermore, through a metagenomic analysis, we shed light on the unknown diversity of methanol utilizers in marine sediments which is yet to be explored.

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Biomarker Indicators of Cold Seeps

Guan

Lei

et al. 2023

South China Sea Seeps

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Lipid biomarkers of seep carbonates and sediments retrieved from the Dongsha area, Shenhu, Site F and Haima in the South China Sea (SCS) over the last two decades were studied. Biomarker inventories, microbial consortia, seepage dynamics, and biogeochemical processes of anaerobic oxidation of methane (AOM), aerobic oxidation of methane (AeOM), and oxidation of non-methane hydrocarbons, were reconstructed. Authigenic carbonates contained varying contents of 13C-depleted archaeal and bacterial biomarkers, reflecting their formation as a result of AOM under varying conditions. Except for the typical isoprenoids found in various cold seeps worldwide, 3,7,11,15-tetramethyl hexadecan-1,3-diol and two novel sn2-/sn3-O-hydroxyphytanyl glycerol monoethers with notable 13C-depletion were observed in authigenic carbonates obtained from Haima, which are most likely hydrolysis products of archaea-specific diethers. Furthermore, molecular fossils, compound-specific δ13C values, and mineralogies, were used to trace dominant microbial consortia, seepage activities, and environmental conditions in the cold seep ecosystems of the SCS. In this chapter, the archaeal and bacterial lipid biomarker geochemistry of methane seeps is systematically introduced. AOM, AeOM, oxidation of non-methane hydrocarbons, oil degradation, and the diagenetic fate of glycerol ethers, are further summarized.

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Biogeochemistry, microbial activity, and diversity in surface and subsurface deep‐sea sediments of South China Sea

Cited by 36 publications

References 99 publications

Active Anaerobic Archaeal Methanotrophs in Recently Emerged Cold Seeps of Northern South China Sea

Active Anaerobic Archaeal Methanotrophs in Recently Emerged Cold Seeps of Northern South China Sea

Anaerobic microbial methanol conversion in marine sediments

Biomarker Indicators of Cold Seeps

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